• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Annexin A1 (ANXA1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476343

Entrez Summary: Annexin I belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35, 000 to 40, 000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
Annexin A1 (ANXA1), Monoclonal Antibody
Full Product Name
Anti-Annexin A1 Mouse mAb
Product Gene Name
anti-ANXA1 antibody
Product Synonym Gene Name
ANX1; LPC1
Product Synonym Names
Annexin 1; Annexin A1; Annexin I (lipocortin I); Annexin I; Annexin-1; AnnexinA1; AnnexinI; ANX 1; ANX A1; ANX1; ANXA 1; ANXA1; ANXA1 protein; ANXA1_HUMAN; Calpactin 2; Calpactin II; Calpactin-2; CalpactinII; Chromobindin 9; Chromobindin-9; Chromobindin9; HGNC:533; Lipocortin 1; Lipocortin I; Lipocortin1; LipocortinI; LPC 1; LPC1; p35; Phospholipase A2 inhibitory protein
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F3-D7-B4
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Molecular Weight
38kDa
OMIM
X05908 mRNA
3D Structure
ModBase 3D Structure for P04083
Immunogen
Purified recombinant fragment of human ANXA1 (AA: 144-248) expressed in E Coli.
Sequence Similarities
Contains 4 annexin repeats.
Subunit Structure
Homodimer; non-covalently linked (By similarity). Homodimer; linked by transglutamylation (PubMed:2532504). Homodimers linked by transglutamylation are observed in placenta, but not in other tissues (PubMed:2532504). Interacts with S100A11 (PubMed:8557678, PubMed:10673436). Heterotetramer, formed by two molecules each of S100A11 and ANXA1 (PubMed:10673436). Interacts with DYSF (By similarity). Interacts with EGFR (By similarity).
Subcellular Location
Cytoplasmic vesicle membrane; Peripheral membrane protein
Applications Tested/Suitable for anti-ANXA1 antibody
Western Blot (WB)

Western Blot (WB) of anti-ANXA1 antibody

Western blot detection of Annexin A1 in MCF7, C6 and Hela cell lysates using Annexin A1 mouse mAb (1:5000 diluted).Predicted band size:98KDa.Observed band size:95KDa.
Mouse Anti-ANXA1 Monoclonal Antibody – Cat# MBS476343

Western Blot (WB) of anti-ANXA1 antibody

Western blot analysis using ANXA1 mouse mAb against Hela (1), A549 (2), K562 (3), NIH3T3 (4), C6 (5), and COS7 (6) cell lysate.
Mouse Anti-ANXA1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476343

Immunohistochemistry (IHC) of anti-ANXA1 antibody

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ANXA1 mouse mAb with DAB staining.
Mouse Anti-ANXA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476343

Immunohistochemistry (IHC) of anti-ANXA1 antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ANXA1 mouse mAb with DAB staining.
Mouse Anti-ANXA1 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476343

Other Notes

Small volumes of anti-ANXA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANXA1 purchase

MBS476343 is an antibody targeting ANXA1. Also known as Anti-Annexin A1 Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ANXA1. It may not necessarily be applicable to this product.

NCBI GI #
4502101
NCBI GeneID
301
NCBI Accession #
NP_000691.1
UniProt Primary Accession #
P04083
UniProt Related Accession #
P04083
GenBank Nucleotide #
NM_000700.2
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
ANXA1
NCBI Official Synonym Symbols
ANX1; LPC1
UniProt Protein Name
Annexin A1
UniProt Synonym Protein Names
Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I
Protein Family
Annexin
UniProt Gene Name
ANXA1
UniProt Synonym Gene Names
ANX1; LPC1
NCBI Protein Information
annexin A1

NCBI Summary for ANXA1

This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors. [provided by RefSeq, Dec 2014]

UniProt Comments for ANXA1

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: apical plasma membrane; cell surface; cell-cell adherens junction; cornified envelope; cytoplasm; early endosome membrane; endosome; extracellular region; extracellular space; extrinsic to external side of plasma membrane; extrinsic to membrane; focal adhesion; lateral plasma membrane; nucleoplasm; nucleus; plasma membrane; vesicle

Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; phospholipase A2 inhibitor activity; phospholipid binding; protein binding; protein binding, bridging; receptor binding; structural molecule activity

Biological Process: actin cytoskeleton reorganization; alpha-beta T cell differentiation; G-protein signaling, coupled to cyclic nucleotide second messenger; inflammatory response; keratinocyte differentiation; monocyte chemotaxis; negative regulation of apoptosis; negative regulation of exocytosis; negative regulation of T-helper 2 cell differentiation; neutrophil homeostasis; peptide cross-linking; phagocytosis; positive regulation of interleukin-2 production; positive regulation of T cell proliferation; positive regulation of T-helper 1 cell differentiation; positive regulation of vesicle fusion; regulation of cell shape; regulation of hormone secretion; regulation of inflammatory response; regulation of interleukin-1 production; regulation of leukocyte migration; signal transduction
Show more

Research Articles on ANXA1

1. Simultaneous knock-down of HIF-1alpha and Annexin A1 (ANXA1), one of the identified genes, resulted in complete cessation of proliferation of gastric cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ANXA1 antibody

 Reference Product  PubMed Publications
 FPR1 antibody  >9 publications with ANXA1 and FPR1
 S100A11 antibody  >5 publications with ANXA1 and S100A11
 SRC antibody  >2 publications with ANXA1 and SRC
 EGFR antibody  >2 publications with ANXA1 and EGFR
 TGM2 antibody  >1 publications with ANXA1 and TGM2

Pathways associated with anti-ANXA1 antibody

 Products by Pathway  Pathway Diagram
 Class A/1 (Rhodopsin-like Receptors) Pathway antibodies  Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
 Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway antibodies  Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway Diagram
 G Alpha (i) Signalling Events Pathway antibodies  G Alpha (i) Signalling Events Pathway Diagram
 G Alpha (q) Signalling Events Pathway antibodies  G Alpha (q) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 GPCR Ligand Binding Pathway antibodies  GPCR Ligand Binding Pathway Diagram
 Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies  Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram
 Integrated Breast Cancer Pathway antibodies  Integrated Breast Cancer Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Peptide Ligand-binding Receptors Pathway antibodies  Peptide Ligand-binding Receptors Pathway Diagram

Diseases associated with anti-ANXA1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >81 publications with ANXA1 and Neoplasms
 Inflammation Antibodies  >60 publications with ANXA1 and Inflammation
 Carcinoma Antibodies  >46 publications with ANXA1 and Carcinoma
 Neoplasm Metastasis Antibodies  >16 publications with ANXA1 and Neoplasm Metastasis
 Brain Diseases Antibodies  >13 publications with ANXA1 and Brain Diseases
 Carcinoma, Squamous Cell Antibodies  >12 publications with ANXA1 and Carcinoma, Squamous Cell
 Necrosis Antibodies  >11 publications with ANXA1 and Necrosis
 Pain Antibodies  >8 publications with ANXA1 and Pain
 Fibrosis Antibodies  >8 publications with ANXA1 and Fibrosis
 Lymphatic Metastasis Antibodies  >7 publications with ANXA1 and Lymphatic Metastasis

Organs/Tissues associated with anti-ANXA1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >72 publications with ANXA1 and Blood
 Brain Antibodies  >22 publications with ANXA1 and Brain
 Vascular Antibodies  >18 publications with ANXA1 and Vascular
 Lung Antibodies  >17 publications with ANXA1 and Lung
 Pituitary Gland Antibodies  >12 publications with ANXA1 and Pituitary Gland
 Liver Antibodies  >11 publications with ANXA1 and Liver
 Lymph Node Antibodies  >10 publications with ANXA1 and Lymph Node
 Muscle Antibodies  >9 publications with ANXA1 and Muscle
 Bone Antibodies  >7 publications with ANXA1 and Bone
 Stomach Antibodies  >6 publications with ANXA1 and Stomach
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000