Twinfilin-1 (TWF1) Antibody – Mouse Monoclonal

This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008]

A6: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: actin cytoskeleton; cell-cell adherens junction; cytoplasm; filopodium; focal adhesion; intercellular junction; myofibril; perinuclear region of cytoplasm

Molecular Function: actin monomer binding; ATP binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity

Biological Process: negative regulation of actin filament polymerization; regulation of actin phosphorylation; sequestering of actin monomers