• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SHP-2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476346

Entrez Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and… More details

DISCONTINUED
Click here to view our similar product(s) OR click here to contact our technical support team for suitable replacement.
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Product Name
SHP-2, Monoclonal Antibody
Full Product Name
Anti-SHP-2 Mouse mAb
Product Gene Name
anti-SHP-2 antibody
Product Synonym Gene Name
PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
Product Synonym Names
BPTP3; CFC; JMML; METCDS; MGC14433; NS1; OTTHUMP00000166107; OTTHUMP00000166108; Protein tyrosine phosphatase 2; Protein tyrosine phosphatase 2C; Protein tyrosine phosphatase non receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11_HUMAN; PTP-1D; PTP-2C; PTP1D; PTP2C; PTPN11; SAP2; SH-PTP2; SH-PTP3; SH2 domain containing protein tyrosine phosphatase 2; SHP 2; SHP-2; Shp2; SHPTP2; SHPTP3; Syp; Tyrosine-protein phosphatase non-receptor type 11
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6G2-H4-F11
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Purified antibody in PBS with 0.05% sodium azide.
Molecular Weight
72kDa
OMIM
D13540 mRNA
3D Structure
ModBase 3D Structure for Q06124
Immunogen
Purified recombinant fragment of human PTPN11 (AA: 263-329) expressed in E Coli.
Sequence Similarities
Contains 1 tyrosine-protein phosphatase domain.
Subunit Structure
Interacts with phosphorylated LIME1 and BCAR3. Interacts with SHB and INPP5D/SHIP1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Interacts with FLT1 (tyrosine-phosphorylated), FLT3 (tyrosine-phosphorylated), FLT4 (tyrosine-phosphorylated), KIT and GRB2. Interacts with PDGFRA (tyrosine phosphorylated). Interacts (via SH2 domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity). Interacts with PTPNS1 and CD84. Interacts with phosphorylated SIT1 and MPZL1. Interacts with FCRL3, FCRL4, FCRL6 and ANKHD1. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts with GAB2. Interacts with TERT; the interaction retains TERT in the nucleus. Interacts with PECAM1 and FER. Interacts with EPHA2 (activated); participates in PTK2/FAK1 dephosphorylation in EPHA2 downstream signaling. Interacts with ROS1; mediates PTPN11 phosphorylation. Interacts with PDGFRB (tyrosine phosphorylated); this interaction increases the PTPN11 phosphatase activity. Interacts with GAREM1 isoform 1 (tyrosine phosphorylated); the interaction increases MAPK/ERK activity and does not affect the GRB2/SOS complex formation. Interacts with CDC73 (PubMed:26742426). Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity).
Subcellular Location
Nucleus
Applications Tested/Suitable for anti-SHP-2 antibody
Western Blot (WB)

Western Blot (WB) of anti-SHP-2 antibody

Western blot detection of SHP-2 in A549, MCF7, C6 and Hela cell lysates using SHP-2 mouse mAb (1:3000 diluted).Predicted band size:68KDa.Observed band size:72KDa.
Mouse Anti-SHP-2 Monoclonal Antibody – Cat# MBS476346

Immunofluorescence (IF) of anti-SHP-2 antibody

Immunofluorescence analysis of HeLa cells using PTPN11 mouse mAb (green). Blue
Mouse Anti-SHP-2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS476346

Flow Cytometry (FC/FACS) of anti-SHP-2 antibody

Flow cytometric analysis of HepG2 cells using PTPN11 mouse mAb (green) and negative control (red).
Mouse Anti-SHP-2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476346

Immunohistochemistry (IHC) of anti-SHP-2 antibody

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PTPN11 mouse mAb with DAB staining.
Mouse Anti-SHP-2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476346

Immunohistochemistry (IHC) of anti-SHP-2 antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PTPN11 mouse mAb with DAB staining.
Mouse Anti-SHP-2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476346

Other Notes

Small volumes of anti-SHP-2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SHP-2 purchase

MBS476346 is an antibody targeting SHP-2. Also known as Anti-SHP-2 Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SHP-2. It may not necessarily be applicable to this product.

NCBI GI #
1059433492
NCBI GeneID
5781
NCBI Accession #
NP_001317366.1
UniProt Primary Accession #
Q06124
UniProt Secondary Accession #
Q96HD7; A8K1D9
UniProt Related Accession #
Q06124
GenBank Nucleotide #
NM_001330437.1
NCBI Official Full Name
tyrosine-protein phosphatase non-receptor type 11 isoform 3
NCBI Official Synonym Full Names
protein tyrosine phosphatase, non-receptor type 11
NCBI Official Symbol
PTPN11
NCBI Official Synonym Symbols
CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 11
UniProt Synonym Protein Names
Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3
Protein Family
Tyrosine-protein phosphatase non-receptor
UniProt Gene Name
PTPN11
UniProt Synonym Gene Names
PTP2C; SHPTP2; PTP-1D; PTP-2C; SHP-2; Shp2
NCBI Protein Information
tyrosine-protein phosphatase non-receptor type 11

NCBI Summary for SHP-2

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for a diversity of cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]
Show more

UniProt Comments for SHP-2

SHP-2: a SH2-containing a ubiquitously expressed tyrosine-specific protein phosphatase. It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens and extracellular matrices in the control of cell growth, differentiation, migration, and death. Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: 1-phosphatidylinositol-3-kinase activity; insulin receptor binding; non-membrane spanning protein tyrosine phosphatase activity; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoprotein phosphatase activity; protein binding; protein tyrosine phosphatase activity; SH3/SH2 adaptor activity

Biological Process: brain development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; genitalia development; heart development; inner ear development; leukocyte migration; phosphoinositide-mediated signaling; platelet activation; regulation of cell adhesion mediated by integrin; regulation of phosphoinositide 3-kinase cascade; regulation of protein complex assembly; T cell costimulation

Disease: Juvenile Myelomonocytic Leukemia; Leopard Syndrome 1; Metachondromatosis; Noonan Syndrome 1
Show more

Research Articles on SHP-2

1. Data indicate that the most prominent proteins associating with Gab2 are PTPN11, PIK3R1 and ARID3B.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SHP-2 antibody

 Reference Product  PubMed Publications
 GRB2 antibody  >144 publications with SHP-2 and GRB2
 JAK2 antibody  >98 publications with SHP-2 and JAK2
 GAB1 antibody  >76 publications with SHP-2 and GAB1
 GAB2 antibody  >45 publications with SHP-2 and GAB2
 IRS1 antibody  >41 publications with SHP-2 and IRS1
 JAK1 antibody  >39 publications with SHP-2 and JAK1
 EGFR antibody  >39 publications with SHP-2 and EGFR
 PDGFRB antibody  >23 publications with SHP-2 and PDGFRB
 FRS2 antibody  >15 publications with SHP-2 and FRS2
 PXN antibody  >14 publications with SHP-2 and PXN

Pathways associated with anti-SHP-2 antibody

 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway antibodies  Activation Of IRF3/IRF7 Mediated By TBK1/IKK Epsilon Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Alpha 6 Beta 4 Signaling Pathway antibodies  Alpha 6 Beta 4 Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram

Diseases associated with anti-SHP-2 antibody

 Disease Name  Pubmed Publications
 Noonan Syndrome Antibodies  >410 publications with SHP-2 and Noonan Syndrome
 Heart Diseases Antibodies  >392 publications with SHP-2 and Heart Diseases
 Leukemia Antibodies  >309 publications with SHP-2 and Leukemia
 LEOPARD Syndrome Antibodies  >137 publications with SHP-2 and LEOPARD Syndrome
 Leukemia, Myelomonocytic, Juvenile Antibodies  >112 publications with SHP-2 and Leukemia, Myelomonocytic, Juvenile
 Carcinoma Antibodies  >86 publications with SHP-2 and Carcinoma
 Inflammation Antibodies  >67 publications with SHP-2 and Inflammation
 Cell Transformation, Neoplastic Antibodies  >57 publications with SHP-2 and Cell Transformation, Neoplastic
 Costello Syndrome Antibodies  >49 publications with SHP-2 and Costello Syndrome
 Adenocarcinoma Antibodies  >44 publications with SHP-2 and Adenocarcinoma

Organs/Tissues associated with anti-SHP-2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >411 publications with SHP-2 and Blood
 Heart Antibodies  >187 publications with SHP-2 and Heart
 Bone Antibodies  >144 publications with SHP-2 and Bone
 Bone Marrow Antibodies  >112 publications with SHP-2 and Bone Marrow
 Muscle Antibodies  >92 publications with SHP-2 and Muscle
 Brain Antibodies  >88 publications with SHP-2 and Brain
 Liver Antibodies  >70 publications with SHP-2 and Liver
 Skin Antibodies  >66 publications with SHP-2 and Skin
 Stomach Antibodies  >50 publications with SHP-2 and Stomach
 Nerve Antibodies  >49 publications with SHP-2 and Nerve
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000