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MARK3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476355

Entrez Summary: The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. More details

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Product Overview

Online Datasheet in PDF
Product Name
MARK3, Monoclonal Antibody
Full Product Name
Anti-MARK3 Mouse mAb
Product Gene Name
Product Synonym Gene Name
KP78; CTAK1; PAR1A; Par-1a
Product Synonym Names
KP78; CTAK1; PAR1A; Par-1a
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2G12
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Molecular Weight
87
OMIM
U64205 mRNA
Immunogen
Purified recombinant fragment of human MARK3 (AA: 435-658) expressed in E Coli.
Applications Tested/Suitable for anti-MARK3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-MARK3 antibody

Western blot analysis using MARK3 mAb against human MARK3 recombinant protein. (Expected MW is 40.8 kDa)
Mouse Anti-MARK3 Monoclonal Antibody – Cat# MBS476355

Western Blot (WB) of anti-MARK3 antibody

Western blot analysis using MARK3 mouse mAb against HeLa (1), SK-N-SH (2), K562 (3), HCT116 (4), HEK293 (5), 3T3L1 (6), NIH3T3 (7), Jurkat (8), and A431 (9) cell lysate.
Mouse Anti-MARK3 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476355

Flow Cytometry (FC/FACS) of anti-MARK3 antibody

Flow cytometric analysis of SK-N-SH cells using MARK3 mouse mAb (green) and negative control (red).
Mouse Anti-MARK3 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476355

Immunohistochemistry (IHC) of anti-MARK3 antibody

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MARK3 mouse mAb with DAB staining.
Mouse Anti-MARK3 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476355

Other Notes

Small volumes of anti-MARK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MARK3 purchase

MBS476355 is an antibody targeting MARK3. Also known as Anti-MARK3 Mouse mAb. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MARK3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
MARK3, partial
NCBI Official Synonym Full Names
microtubule affinity regulating kinase 3
NCBI Official Symbol
MARK3
NCBI Official Synonym Symbols
KP78; CTAK1; PAR1A; Par-1a
UniProt Protein Name
MAP/microtubule affinity-regulating kinase 3
UniProt Synonym Protein Names
C-TAK1; cTAK1; Cdc25C-associated protein kinase 1; ELKL motif kinase 2; EMK-2; Protein kinase STK10; Ser/Thr protein kinase PAR-1; Par-1a; Serine/threonine-protein kinase p78
UniProt Gene Name
MARK3
UniProt Synonym Gene Names
CTAK1; EMK2; cTAK1; EMK-2; Par-1a
NCBI Protein Information
MAP/microtubule affinity-regulating kinase 3

NCBI Summary for MARK3

The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

UniProt Comments for MARK3

MARK3: an ubiquitous serine/threonine kinase of the CAMKL family. Phosphorylates Cdc25C on serine 216 and promotes 14-3-3 protein binding. A positive regulator of Wnt signalling. Ubiquitously expressed in human tissues and cell lines. Seven alternatively spliced isoforms have been reported.

Protein type: CAMK group; CAMKL family; EC 2.7.11.1; Kinase, protein; MARK subfamily; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 14q32.32-q32.33

Cellular Component: cytosol; plasma membrane

Molecular Function: protein binding; protein serine/threonine kinase activity

Biological Process: MAPKKK cascade
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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