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PIWIL4 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476393

Entrez Summary: PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
PIWIL4, Monoclonal Antibody
Full Product Name
Anti-PIWIL4 Mouse mAb
Product Gene Name
anti-PIWIL4 antibody
Product Synonym Gene Name
HIWI2; MIWI2
Product Synonym Names
HIWI2; MIWI2
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10G9B11
Host
Mouse
Species Reactivity
Human
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Molecular Weight
96.6
OMIM
AB079366 mRNA
3D Structure
ModBase 3D Structure for Q7Z3Z4
Immunogen
Purified recombinant fragment of human PIWIL4 (AA: 304-434) expressed in E Coli.
Applications Tested/Suitable for anti-PIWIL4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Western Blot (WB) of anti-PIWIL4 antibody

Western blot analysis using PIWIL4 mAb against human PIWIL4 recombinant protein. (Expected MW is 40.8 kDa)
Mouse Anti-PIWIL4 Monoclonal Antibody – Cat# MBS476393

Western Blot (WB) of anti-PIWIL4 antibody

Western blot analysis using PIWIL4 mAb against HEK293 (1) and PIWIL4 (AA
Mouse Anti-PIWIL4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476393

Western Blot (WB) of anti-PIWIL4 antibody

Western blot analysis using PIWIL4 mouse mAb against PC-3 (1) and PANC-1 (2) cell lysate.
Mouse Anti-PIWIL4 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476393

Flow Cytometry (FC/FACS) of anti-PIWIL4 antibody

Flow cytometric analysis of HeLa cells using PIWIL4 mouse mAb (green) and negative control (red).
Mouse Anti-PIWIL4 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476393

Immunohistochemistry (IHC) of anti-PIWIL4 antibody

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 mouse mAb with DAB staining.
Mouse Anti-PIWIL4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476393

Immunohistochemistry (IHC) of anti-PIWIL4 antibody

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 mouse mAb with DAB staining.
Mouse Anti-PIWIL4 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476393

Other Notes

Small volumes of anti-PIWIL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PIWIL4 purchase

MBS476393 is an antibody targeting PIWIL4. Also known as Anti-PIWIL4 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PIWIL4. It may not necessarily be applicable to this product.

NCBI GI #
221316711
NCBI GeneID
143689
NCBI Accession #
NP_689644.2
UniProt Primary Accession #
Q7Z3Z4
UniProt Secondary Accession #
Q68CZ4; Q8N8G9; Q8N9V8; Q8NEH2; B4DEG5
UniProt Related Accession #
Q7Z3Z4
GenBank Nucleotide #
NM_152431.2
NCBI Official Full Name
piwi-like protein 4
NCBI Official Synonym Full Names
piwi like RNA-mediated gene silencing 4
NCBI Official Symbol
PIWIL4
NCBI Official Synonym Symbols
HIWI2; MIWI2
UniProt Protein Name
Piwi-like protein 4
Protein Family
Piwi-like protein
UniProt Gene Name
PIWIL4
UniProt Synonym Gene Names
HIWI2; PIWI
NCBI Protein Information
piwi-like protein 4

NCBI Summary for PIWIL4

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

UniProt Comments for PIWIL4

PIWIL4: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 11q21

Cellular Component: cytoplasm; nucleus

Biological Process: DNA methylation during gametogenesis; RNA-mediated gene silencing; spermatogenesis
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Research Articles on PIWIL4

1. PIWIL4 is highly expressed in both breast cancer tissues and the cytoplasm of MDA-MB-231 cells derived from breast cancer. Reducing PIWIL4 expression drastically impairs the migration ability of MDA-MB-231 cells, significantly increases their apoptosis, and mildly affects their proliferation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PIWIL4 antibody

 Reference Product  PubMed Publications
 TDRD1 antibody  >6 publications with PIWIL4 and TDRD1
 EIF2C2 antibody  >3 publications with PIWIL4 and EIF2C2
 MAEL antibody  >2 publications with PIWIL4 and MAEL
 TDRD9 antibody  >2 publications with PIWIL4 and TDRD9
 TDRKH antibody  >1 publications with PIWIL4 and TDRKH
 MOV10L1 antibody  >1 publications with PIWIL4 and MOV10L1

Pathways associated with anti-PIWIL4 antibody

 Products by Pathway  Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Dorso-ventral Axis Formation Pathway antibodies  Dorso-ventral Axis Formation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 PIWI-interacting RNA (piRNA) Biogenesis Pathway antibodies  PIWI-interacting RNA (piRNA) Biogenesis Pathway Diagram
 Regulatory RNA Pathways antibodies  Regulatory RNA Pathways Diagram

Diseases associated with anti-PIWIL4 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >2 publications with PIWIL4 and Neoplasm Metastasis
 Gastrointestinal Diseases Antibodies  >2 publications with PIWIL4 and Gastrointestinal Diseases
 Neoplasm Invasiveness Antibodies  >1 publications with PIWIL4 and Neoplasm Invasiveness
 Carcinoma, Hepatocellular Antibodies  >1 publications with PIWIL4 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >1 publications with PIWIL4 and Liver Neoplasms
 Uterine Cervical Neoplasms Antibodies  >1 publications with PIWIL4 and Uterine Cervical Neoplasms
 Liver Diseases Antibodies  >1 publications with PIWIL4 and Liver Diseases
 Oligospermia Antibodies  >1 publications with PIWIL4 and Oligospermia

Organs/Tissues associated with anti-PIWIL4 antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >6 publications with PIWIL4 and Testis
 Ovary Antibodies  >3 publications with PIWIL4 and Ovary
 Blood Antibodies  >2 publications with PIWIL4 and Blood
 Liver Antibodies  >2 publications with PIWIL4 and Liver
 Heart Antibodies  >1 publications with PIWIL4 and Heart
 Nerve Antibodies  >1 publications with PIWIL4 and Nerve
 Brain Antibodies  >1 publications with PIWIL4 and Brain
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