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TUBB2A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476686

Entrez Summary: Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Product Name
TUBB2A, Monoclonal Antibody
Full Product Name
Anti-TUBB2A Mouse mAb
Product Gene Name
Product Synonym Gene Name
TUBB; TUBB2; CDCBM5
Product Synonym Names
Class IIa beta tubulin; dJ40E16.7; M (beta)2; TBB2A_HUMAN; TUBB; TUBB2; Tubb2a; Tubulin beta 2; Tubulin beta 2A; Tubulin beta 2A class IIa; Tubulin beta polypeptide 2; Tubulin beta polypeptide; Tubulin beta-2A chain
Clonality
Monoclonal
Clone Number
2H12-H3-F2
Host
Mouse
Species Reactivity
Human, Rat
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Molecular Weight
50kDa
OMIM
X79535 mRNA
3D Structure
ModBase 3D Structure for Q13885
Immunogen
Purified recombinant fragment of human TUBB2A expressed in E Coli.
Sequence Similarities
Belongs to the tubulin family.
Subunit Structure
Interacts with ZNRF1 (By similarity). Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity (By similarity). Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Subcellular Location
Cytoplasm; Cytoskeleton
Applications Tested/Suitable for anti-TUBB2A antibody
Western Blot (WB)

Western Blot (WB) of anti-TUBB2A antibody

Western blot detection of TUBB2A in Jurkat, A549, MCF7 and C6 cell lysates using TUBB2A mouse mAb (1:3000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
Mouse Anti-TUBB2A Monoclonal Antibody – Cat# MBS476686

Western Blot (WB) of anti-TUBB2A antibody

Western blot analysis using TUBB2A mAb against HEK293 (1) and TUBB2A (AA
Mouse Anti-TUBB2A Monoclonal Antibody, Western Blot (WB) – Cat# MBS476686

Western Blot (WB) of anti-TUBB2A antibody

Western blot analysis using TUBB2A mouse mAb against HeLa (1), A549 (2), HEK293 (3), Jurkat (4) and PC-12 (5) cell lysate.
Mouse Anti-TUBB2A Monoclonal Antibody, Western Blot (WB) – Cat# MBS476686

Flow Cytometry (FC/FACS) of anti-TUBB2A antibody

Flow cytometric analysis of Hela cells using TUBB2A mouse mAb (green) and negative control (red).
Mouse Anti-TUBB2A Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476686

Other Notes

Small volumes of anti-TUBB2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TUBB2A purchase

MBS476686 is an antibody targeting TUBB2A. Also known as Anti-TUBB2A Mouse mAb. Reactive in Human, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TUBB2A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
tubulin beta-2A chain isoform 1
NCBI Official Synonym Full Names
tubulin beta 2A class IIa
NCBI Official Symbol
TUBB2A
NCBI Official Synonym Symbols
TUBB; TUBB2; CDCBM5
UniProt Protein Name
Tubulin beta-2A chain
UniProt Synonym Protein Names
Tubulin beta class IIa
Protein Family
UniProt Gene Name
TUBB2A
UniProt Synonym Gene Names
TUBB2
NCBI Protein Information
tubulin beta-2A chain

NCBI Summary for TUBB2A

Microtubules, key participants in processes such as mitosis and intracellular transport, are composed of heterodimers of alpha- and beta-tubulins. The protein encoded by this gene is a beta-tubulin. Defects in this gene are associated with complex cortical dysplasia with other brain malformations-5. Two transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
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UniProt Comments for TUBB2A

TUBB2A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Belongs to the tubulin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p25.2

Cellular Component: microtubule; nucleus

Molecular Function: protein binding

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 5
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TUBB2A antibody

 Reference Product  PubMed Publications
 TUBA1A antibody  >2 publications with TUBB2A and TUBA1A
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