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Cyclin A2 (CCNA2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476775

Entrez Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and… More details

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Product Overview

Online Datasheet in PDF
Product Name
Cyclin A2 (CCNA2), Monoclonal Antibody
Full Product Name
Anti-Cyclin A2 Mouse mAb
Product Gene Name
Product Synonym Gene Name
CCN1; CCNA
Product Synonym Names
CCN1; CCNA; Ccna2; CCNA2_HUMAN; Cyclin A2; Cyclin-A; Cyclin-A2
Clonality
Monoclonal
Clone Number
1G1-C7-F9
Host
Mouse
Species Reactivity
Human
Form/Format
Purified antibody in PBS with 0.05% sodium azide.
Molecular Weight
50kDa
OMIM
X51688 mRNA
3D Structure
ModBase 3D Structure for P20248
Immunogen
Purified recombinant fragment of human CCNA2 (AA: 105-233) expressed in E Coli.
Sequence Similarities
Belongs to the cyclin family. Cyclin AB subfamily.
Subunit Structure
Interacts with the CDK1 and CDK2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. When associated with CDK2 (but not with CDK1), interacts with SCAPER. Interacts with human cytomegalovirus protein UL32 (PubMed:24101496).
Subcellular Location
Cytoplasm
Applications Tested/Suitable for anti-CCNA2 antibody
Western Blot (WB)

Western Blot (WB) of anti-CCNA2 antibody

Western blot detection of Cyclin A2 in Jurkat cell lysates using Cyclin A2 mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
Mouse Anti-CCNA2 Monoclonal Antibody – Cat# MBS476775

Western Blot (WB) of anti-CCNA2 antibody

Western blot analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA
Mouse Anti-CCNA2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476775

Western Blot (WB) of anti-CCNA2 antibody

Western blot analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.
Mouse Anti-CCNA2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS476775

Flow Cytometry (FC/FACS) of anti-CCNA2 antibody

Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).
Mouse Anti-CCNA2 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS476775

Immunohistochemistry (IHC) of anti-CCNA2 antibody

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.
Mouse Anti-CCNA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476775

Immunohistochemistry (IHC) of anti-CCNA2 antibody

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.
Mouse Anti-CCNA2 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476775

Other Notes

Small volumes of anti-CCNA2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCNA2 purchase

MBS476775 is an antibody targeting CCNA2. Also known as Anti-Cyclin A2 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CCNA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cyclin-A2
NCBI Official Synonym Full Names
cyclin A2
NCBI Official Symbol
CCNA2
NCBI Official Synonym Symbols
CCN1; CCNA
UniProt Protein Name
Cyclin-A2
UniProt Synonym Protein Names
Cyclin A
Protein Family
UniProt Gene Name
CCNA2
UniProt Synonym Gene Names
Cyclin-A
NCBI Protein Information
cyclin-A2

NCBI Summary for CCNA2

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members function as regulators of the cell cycle. This protein binds and activates cyclin-dependent kinase 2 and thus promotes transition through G1/S and G2/M. [provided by RefSeq, Aug 2016]

UniProt Comments for CCNA2

CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: cytoplasm; Golgi apparatus; nucleoplasm; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: G2/M transition of mitotic cell cycle; Ras protein signal transduction
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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