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Cadherin 17/Li Cadherin (CDH17) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4380773

MBS4380773 is an antibody targeting CDH17. Also known as Cadherin 17/LI Cadherin (Liver-Intestine Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cadherin 17/LI Cadherin (CDH17), Monoclonal Antibody
Full Product Name
Cadherin 17/LI Cadherin (Liver-Intestine Marker)
Product Gene Name
Product Synonym Names
BILL-cadherin; Cadherin-17; CDH17; HPT-1 cadherin; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1 (HPT-1); Intestinal peptide-associated transporter HPT-1; LI-cadherin (liver-intestine); Liver Cadherin; Liver-intestine cadherin
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
CDH17/2618
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Specificity
It recognizes a protein of 120kDa, which is identified as Cadherin 17 (also known as LI Cadherin). The cadherins are a family of Calcium-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. LI-cadherin (for liver-intestine-cadherin) expression is restricted to liver and intestine tissues and is specifically localized to the basolateral domain of hepatocytes and enterocytes.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Non-hazardous. No MSDS required.
Molecular Weight
120kDa
OMIM
603017
3D Structure
ModBase 3D Structure for Q12864
Positive Control
MCF-7, HepG2 or HT29 cells. Human Stomach, Small Intestine, Colon or liver.
Immunogen
Recombinant fragment (around aa 242-418) of human Cadherin 17 protein (CDH17) (exact sequence is proprietary)
Cellular Localization
Cell Membrane
Applications Tested/Suitable for anti-CDH17 antibody
Flow Cytometry (FC), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-CDH17 antibody
FC: 1-2ug/million cells
WB: 2-4 ug/ml
IHC- Formalin-fixed: 1-2ug/ml for 30 mins at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-CDH17 antibody

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618).
Mouse Anti-CDH17 Monoclonal Antibody – Cat# MBS4380773

SDS-Page of anti-CDH17 antibody

SDS-PAGE Analysis
Purified Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618). Confirmation of Purity and Integrity of Antibody
Mouse Anti-CDH17 Monoclonal Antibody, SDS-Page – Cat# MBS4380773

Flow Cytometry (FC/FACS) of anti-CDH17 antibody

Flow Cytometric Analysis of MCF-7 cells using Cadherin 17/CDH17 Mouse Monoclonal Antibody (CDH17/2618) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-CDH17 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS4380773

Testing Data of anti-CDH17 antibody

Analysis of Protein Array containing more than 19,000 full-length human proteins using Cadherin 17 (CDH17) Mouse Monoclonal Antibody (CDH17/2618). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Mouse Anti-CDH17 Monoclonal Antibody, Testing Data – Cat# MBS4380773

Western Blot (WB) of anti-CDH17 antibody

Western Blot Analysis of human Small Intestine tissue lysate using CDH17 Mouse Monoclonal Antibody (CDH17/2618).
Mouse Anti-CDH17 Monoclonal Antibody, Western Blot (WB) – Cat# MBS4380773

Product References and Citations for anti-CDH17 antibody

Berndorff, D., Gessner, R., Kreft, B., Schnoy, N., Lajours-Petter, A.M., Loch, N., Reutter, W., Hortsch, M. and Tauber, R. 1994. Liver-intestine cadherin: molecular cloning and characterization of a novel Ca2+-dependent cell adhesion molecule expressed in liver and intestine. J. Cell Biol. 125: 1353-1369.

Other Notes

Small volumes of anti-CDH17 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDH17. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cadherin-17
NCBI Official Synonym Full Names
cadherin 17
NCBI Official Symbol
CDH17
NCBI Official Synonym Symbols
HPT1; CDH16; HPT-1
UniProt Protein Name
Cadherin-17
UniProt Synonym Protein Names
Intestinal peptide-associated transporter HPT-1; Liver-intestine cadherin
Protein Family
UniProt Gene Name
CDH17
UniProt Synonym Gene Names
LI-cadherin
UniProt Entry Name
CAD17_HUMAN
NCBI Protein Information
cadherin-17

NCBI Summary for CDH17

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]
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UniProt Comments for CDH17

CDH17: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: transporter activity; calcium ion binding; proton-dependent oligopeptide secondary active transmembrane transporter activity

Biological Process: intercellular junction assembly and maintenance; transport; calcium-dependent cell-cell adhesion; oligopeptide transport; homophilic cell adhesion; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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