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Histone H3 (HIST1H3A) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476959

Entrez Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of… More details

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Product Overview

Product Name
Histone H3 (HIST1H3A), Polyclonal Antibody
Full Product Name
Anti-Phospho-Histone H3 (Ser10) Rabbit pAb
Product Gene Name
anti-HIST1H3A antibody
Product Synonym Gene Name
H3/A; H3FA
Product Synonym Names
Histone H3/f; Histone H3; Histone H3/d; Histone H3/b; Histone H3/c; Histone H3/a; Histone H3/l; Histone H3/j; Histone H3/k; Histone H3/h; H3/A; H3FA; Histone H3/i
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Sensitivity
This antibody detects endogenous levels of Phospho-Histone H3 (Ser10) and does not cross-react with related proteins.
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Molecular Weight
17kDa
OMIM
X00090 Genomic DNA
3D Structure
ModBase 3D Structure for P68431
Immunogen
Human Phospho-Histone H3 (Ser10) synthetic peptide conjugated to KLH.
Sequence Similarities
Belongs to the histone H3 family.
Subunit Structure
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
Subcellular Location
Nucleus Chromosome

Product Categories/Family for anti-HIST1H3A antibody

Epigenetics and Nuclear Signaling; Histones; H3; Acetylated; Epigenetics and Nuclear Signaling; ChIP'ing Antibodies; ChIP'ing Antibodies
Applications Tested/Suitable for anti-HIST1H3A antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-HIST1H3A antibody
WB: 1:1000
ICC: 1:200

Western Blot (WB) of anti-HIST1H3A antibody

Western blot analysis of extracts from HeLa cells, untreated or 1mM hydroxyurea treated for 5 hours, using Phospho-Histone H3 (Ser10) Rabbit pAb (1:1000 diluted).
Rabbit Anti-HIST1H3A Polyclonal Antibody – Cat# MBS476959

Immunofluorescence (IF) of anti-HIST1H3A antibody

Immunofluorescent analysis of Hela cells labeled with Phospho-Histone H3 (Ser10) (dilution 1:200) Rabbit pAb. DAPI was used to stain nucleus (blue).
Rabbit Anti-HIST1H3A Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS476959

Other Notes

Small volumes of anti-HIST1H3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIST1H3A purchase

MBS476959 is an antibody targeting HIST1H3A. Also known as Anti-Phospho-Histone H3 (Ser10) Rabbit pAb. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H3A. It may not necessarily be applicable to this product.

NCBI GI #
4504281
NCBI GeneID
8350
NCBI Accession #
NP_003520.1
UniProt Primary Accession #
P68431
UniProt Secondary Accession #
P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
UniProt Related Accession #
P68431
GenBank Nucleotide #
NM_003529.2
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1 H3 family member a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
Protein Family
Histone H3-like centromeric protein
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
NCBI Protein Information
histone H3.1

NCBI Summary for HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
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UniProt Comments for HIST1H3A

H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p22.2

Cellular Component: cell-cell adherens junction; extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex

Molecular Function: histone binding; nucleosomal DNA binding; protein binding

Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis
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Research Articles on HIST1H3A

1. AS1eRNA-driven DNA looping and activating histone modifications promote the expression of DHRS4-AS1 to economically control the DHRS4 gene cluster.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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