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4E-BP1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476961

Entrez Summary: This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S… More details

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Product Overview

Product Name
4E-BP1, Polyclonal Antibody
Full Product Name
Anti-4E-BP1 Rabbit pAb
Product Gene Name
anti-4E-BP1 antibody
Product Synonym Gene Name
BP-1; 4EBP1; 4E-BP1; PHAS-I; EIF4EBP1
Product Synonym Names
4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Sensitivity
4E-BP1 Rabbit pAb detects endogenous levels of total 4E-BP1 protein.
Form/Format
Purified rabbit polyclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Purity/Purification
Affinity Purified
Concentration
0.928mg/ml (lot specific)
Molecular Weight
15-20KDa
OMIM
L36055 mRNA
3D Structure
ModBase 3D Structure for Q13541
Immunogen
Synthetic peptide.
Sequence Similarities
Belongs to the eIF4E-binding protein family.
Subunit Structure
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts (via TOS motif) with RPTOR; promoting phosphorylation by mTORC1 (PubMed:12747827).
Subcellular Location
Cytoplasm.
Applications Tested/Suitable for anti-4E-BP1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-4E-BP1 antibody
WB: 1:1000

Western Blot (WB) of anti-4E-BP1 antibody

Western blot detection of 4E-BP1 in SHSY-5Y, C2C12 and 3T3 cell lysates using 4E-BP1 Rabbit pAb (1:1000 diluted). Predicted band size: 13KDa. Observed band size:15~20KDa.
Rabbit Anti-4E-BP1 Polyclonal Antibody – Cat# MBS476961

Immunoprecipitation (IP) of anti-4E-BP1 antibody

Immunoprecipitation analysis of Hela cell lysates using 4E-BP1 Rabbit pAb.
Rabbit Anti-4E-BP1 Polyclonal Antibody, Immunoprecipitation (IP) – Cat# MBS476961

Western Blot (WB) of anti-4E-BP1 antibody

Western blot detection of 4E-BP1 in Jurkat, Hela and C6 cell lysates using 4E-BP1 Rabbit pAb (1:1000 diluted). Predicted band size: 13KDa. Observed band size:15~20KDa.
Rabbit Anti-4E-BP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS476961

Western Blot (WB) of anti-4E-BP1 antibody

Western blot detection of 4E-BP1 in C2C12, A549 and 3T3 cell lysates using 4E-BP1 Rabbit pAb (1:1000 diluted). Predicted band size: 13KDa. Observed band size:15~20KDa.
Rabbit Anti-4E-BP1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS476961

Other Notes

Small volumes of anti-4E-BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for 4E-BP1 purchase

MBS476961 is an antibody targeting 4E-BP1. Also known as Anti-4E-BP1 Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for 4E-BP1. It may not necessarily be applicable to this product.

NCBI GI #
4758258
NCBI GeneID
1978
NCBI Accession #
NP_004086.1
UniProt Primary Accession #
Q13541
UniProt Secondary Accession #
Q6IBN3; B2R502; D3DSW8
UniProt Related Accession #
Q13541
GenBank Nucleotide #
NM_004095.3
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Protein Family
Human immunodeficiency virus type I enhancer-binding protein
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1

NCBI Summary for 4E-BP1

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
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UniProt Comments for 4E-BP1

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p11.23

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: eukaryotic initiation factor 4E binding; protein binding; translation repressor activity

Biological Process: G1/S transition of mitotic cell cycle; negative regulation of translational initiation; positive regulation of mitotic cell cycle; TOR signaling pathway
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Research Articles on 4E-BP1

1. Increased expression of miR-125a is associated with invasion and migration in ovarian cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-4E-BP1 antibody

 Reference Product  PubMed Publications
 EIF4E antibody  >460 publications with 4E-BP1 and EIF4E
 MTOR antibody  >444 publications with 4E-BP1 and MTOR
 AKT1 antibody  >49 publications with 4E-BP1 and AKT1
 MAPK1 antibody  >22 publications with 4E-BP1 and MAPK1
 ATM antibody  >11 publications with 4E-BP1 and ATM
 RPS6KB1 antibody  >3 publications with 4E-BP1 and RPS6KB1
 MAPK14 antibody  >2 publications with 4E-BP1 and MAPK14
 EIF4E2 antibody  >2 publications with 4E-BP1 and EIF4E2
 BUB1 antibody  >1 publications with 4E-BP1 and BUB1

Pathways associated with anti-4E-BP1 antibody

 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Acute Myeloid Leukemia Pathway antibodies  Acute Myeloid Leukemia Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram
 ErbB Signaling Pathway antibodies  ErbB Signaling Pathway Diagram

Diseases associated with anti-4E-BP1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >241 publications with 4E-BP1 and Neoplasms
 Hypertrophy Antibodies  >40 publications with 4E-BP1 and Hypertrophy
 Breast Neoplasms Antibodies  >38 publications with 4E-BP1 and Breast Neoplasms
 Cardiovascular Diseases Antibodies  >34 publications with 4E-BP1 and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >31 publications with 4E-BP1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >20 publications with 4E-BP1 and Neoplasms, Experimental
 Drug Toxicity Antibodies  >18 publications with 4E-BP1 and Drug Toxicity
 Kidney Diseases Antibodies  >16 publications with 4E-BP1 and Kidney Diseases
 Necrosis Antibodies  >16 publications with 4E-BP1 and Necrosis
 Leukemia, Myeloid, Acute Antibodies  >16 publications with 4E-BP1 and Leukemia, Myeloid, Acute

Organs/Tissues associated with anti-4E-BP1 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >161 publications with 4E-BP1 and Muscle
 Blood Antibodies  >110 publications with 4E-BP1 and Blood
 Liver Antibodies  >46 publications with 4E-BP1 and Liver
 Vascular Antibodies  >44 publications with 4E-BP1 and Vascular
 Heart Antibodies  >40 publications with 4E-BP1 and Heart
 Brain Antibodies  >40 publications with 4E-BP1 and Brain
 Kidney Antibodies  >34 publications with 4E-BP1 and Kidney
 Lung Antibodies  >33 publications with 4E-BP1 and Lung
 Pancreas Antibodies  >21 publications with 4E-BP1 and Pancreas
 Prostate Antibodies  >17 publications with 4E-BP1 and Prostate
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