Rabbit Anti-IKKa/b Polyclonal Antibody – Cat# MBS477146

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using IKKa/b (Phospho-Ser180/181) Antibody.

IKKa/b Antibody – Rabbit Polyclonal

Name: IKKa/b Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS477146
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477146

Entrez Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates… More details

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Product Documentation

Sample Manual Insert View PDF

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Material Safety Data Sheet View PDF

Technical Datasheet View PDF

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Product Overview

Product Name

IKKa/b, Polyclonal Antibody

Full Product Name

Anti-IKKa/b (Phospho-Ser180/181) Rabbit pAb

Product Gene Name

anti-IKKa/b antibody

Product Synonym Gene Name

IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha /IKK2; IKKB; IMD15; NFKBIKB; IKK-beta

Product Synonym Names

chuk; CHUK1; Conserved Helix Loop Helix Ubiquitous Kinase; Conserved helix loop ubiquitous kinase; Conserved helix-loop-helix ubiquitous kinase; I Kappa B Kinase 1; I Kappa B Kinase Alpha; I-kappa-B kinase 1; I-kappa-B kinase alpha; IkappaB kinase; IkB kinase alpha subunit; IKBKA; IKK 1; IKK A; IKK a kinase; IKK-A; IKK-alpha; IKK1; IKKA; IKKA_HUMAN; Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells; Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit; Inhibitor of nuclear factor kappa-B kinase subunit alpha; NFKBIKA; Nuclear Factor Kappa B Inhibitor Kinase Alpha; Nuclear factor NF kappa B inhibitor kinase alpha; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor; TCF-16; TCF16; Transcription factor 16. / I kappa B kinase 2; I kappa B kinase beta; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK 2; IKK B; IKK beta; IKK-B; IKK-beta; IKK2; IKKB; IKKB_HUMAN; Inhibitor of kappa light chain gene enhancer in B cells; Inhibitor of kappa light polypeptide gene enhancer in B cells; Inhibitor of kappa light polypeptide gene enhancer in B cells kinase beta; Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta; Inhibitor of nuclear factor kappa B kinase beta subunit; Inhibitor of nuclear factor kappa B kinase subunit beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; MGC131801; NFKBIKB; Nuclear factor NF kappa B inhibitor kinase beta; Nuclear factor NF-kappa-B inhibitor kinase beta; Nuclear factor of kappa light chain gene enhancer in B cells inhibitor

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit
Source: Human

Species Reactivity

Human, Mouse, Rat

Sensitivity

This antibody detects endogenous levels of IKKa/b protein.

Form/Format

10mM PBS with 0.02% sodium azide, 50% glycerol.

Purity/Purification

Affinity Purified

Molecular Weight

84; 87

OMIM

AF012890 mRNA

3D Structure

ModBase 3D Structure for O15111

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human IKKa/b around the phosphorylation site of serine 180/181 (C-T-SP-F-V).

Sequence Similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.Contains 1 protein kinase domain.

Subunit Structure

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Directly interacts with IKK-gamma/NEMO and TRPC4AP. May interact with TRAF2. Interacts with NALP2. May interact with MAVS/IPS1. Interacts with ARRB1 and ARRB2. Interacts with NLRC5; prevents CHUK phosphorylation and kinase activity. Interacts with PIAS1; this interaction induces PIAS1 phosphorylation. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner. / Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. The IKK complex associates with TERF2IP/RAP1, leading to promote IKK-mediated phosphorylation of RELA/p65. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Found in a membrane raft complex, at least composed of BCL10, CARD11, DPP4 and IKBKB. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with Yersinia yopJ. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct. Interacts with ARRB1 and ARRB2. Interacts with TRIM21. Interacts with NLRC5; prevents IKBKB phosphorylation and kinase activity. Interacts with PDPK1. Interacts with EIF2AK2/PKR. The phosphorylated form interacts with PPM1A and PPM1B. Interacts with ZNF268 isoform 2; the interaction is further increased in a TNF-alpha-dependent manner.

Subcellular Location

Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and the nucleus. /Cytoplasm. Nucleus. Membrane raft. Note: Colocalized with DPP4 in membrane rafts.

Applications Tested/Suitable for anti-IKKa/b antibody

Western Blot (WB), Immunohistochemistry (IHC)

Other Notes

Small volumes of anti-IKKa/b antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IKKa/b purchase

MBS477146 is an antibody targeting IKKa/b. Also known as Anti-IKKa/b (Phospho-Ser180/181) Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for IKKa/b. It may not necessarily be applicable to this product.

NCBI GI #

62241001

NCBI GeneID

1147

NCBI Accession #

NP_001269.3

UniProt Primary Accession #

O15111

UniProt Secondary Accession #

O14666; Q13132; Q5W0I4; Q92467

UniProt Related Accession #

O15111

GenBank Nucleotide #

NM_001278.3

NCBI Official Full Name

inhibitor of nuclear factor kappa-B kinase subunit alpha isoform 1

NCBI Official Synonym Full Names

conserved helix-loop-helix ubiquitous kinase

NCBI Official Symbol

CHUK

NCBI Official Synonym Symbols

IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

UniProt Protein Name

Inhibitor of nuclear factor kappa-B kinase subunit alpha

UniProt Synonym Protein Names

Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16

UniProt Gene Name

CHUK

UniProt Synonym Gene Names

IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16

NCBI Protein Information

inhibitor of nuclear factor kappa-B kinase subunit alpha

NCBI Summary for IKKa/b

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

UniProt Comments for IKKa/b

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: EC 2.7.11.10; IKK family; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: cytoplasm; cytosol; IkappaB kinase complex; internal side of plasma membrane; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: IkappaB kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity

Biological Process: activation of NF-kappaB transcription factor; anatomical structure morphogenesis; I-kappaB kinase/NF-kappaB cascade; I-kappaB phosphorylation; immune response; inflammatory response; innate immune response; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; response to virus; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway

Disease: Cocoon Syndrome

Research Articles on IKKa/b

1. Data indicate a significantly higher quantity of mesenchymal stromal cells (MSCs)was produced from human embryonic stem cells (hESCs) with IkappaB kinase (IKK)/nuclear factor kappa B (NF-kappaB) suppression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-IKKa/b antibody

Reference Product	PubMed Publications
IKBKB antibody	>1157 publications with IKKa/b and IKBKB
RELA antibody	>152 publications with IKKa/b and RELA
TRAF2 antibody	>69 publications with IKKa/b and TRAF2
AKT1 antibody	>39 publications with IKKa/b and AKT1
CCND1 antibody	>33 publications with IKKa/b and CCND1
CASP8 antibody	>26 publications with IKKa/b and CASP8
NFKB2 antibody	>18 publications with IKKa/b and NFKB2
BCL10 antibody	>18 publications with IKKa/b and BCL10
TANK antibody	>16 publications with IKKa/b and TANK
SRC antibody	>15 publications with IKKa/b and SRC

Pathways associated with anti-IKKa/b antibody

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
AKT Phosphorylates Targets In The Cytosol Pathway antibodies	AKT Phosphorylates Targets In The Cytosol Pathway Diagram
Activated TLR4 Signalling Pathway antibodies	Activated TLR4 Signalling Pathway Diagram
Activation Of NF-kappaB In B Cells Pathway antibodies	Activation Of NF-kappaB In B Cells Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Acute Myeloid Leukemia Pathway antibodies	Acute Myeloid Leukemia Pathway Diagram
Adaptive Immune System Pathway antibodies	Adaptive Immune System Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Adipocytokine Signaling Pathway antibodies	Adipocytokine Signaling Pathway Diagram
Alternative NF-kappaB Pathway antibodies	Alternative NF-kappaB Pathway Diagram

Diseases associated with anti-IKKa/b antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>478 publications with IKKa/b and Necrosis
Inflammation Antibodies	>233 publications with IKKa/b and Inflammation
Neoplasms Antibodies	>208 publications with IKKa/b and Neoplasms
Skin Abnormalities Antibodies	>39 publications with IKKa/b and Skin Abnormalities
Abnormalities, Multiple Antibodies	>36 publications with IKKa/b and Abnormalities, Multiple
Heart Diseases Antibodies	>33 publications with IKKa/b and Heart Diseases
Cell Transformation, Neoplastic Antibodies	>28 publications with IKKa/b and Cell Transformation, Neoplastic
Liver Diseases Antibodies	>20 publications with IKKa/b and Liver Diseases
Adenocarcinoma Antibodies	>18 publications with IKKa/b and Adenocarcinoma
Liver Neoplasms Antibodies	>14 publications with IKKa/b and Liver Neoplasms

Organs/Tissues associated with anti-IKKa/b antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>221 publications with IKKa/b and Blood
Lung Antibodies	>71 publications with IKKa/b and Lung
Skin Antibodies	>63 publications with IKKa/b and Skin
Liver Antibodies	>55 publications with IKKa/b and Liver
Heart Antibodies	>50 publications with IKKa/b and Heart
Bone Antibodies	>49 publications with IKKa/b and Bone
Muscle Antibodies	>37 publications with IKKa/b and Muscle
Kidney Antibodies	>35 publications with IKKa/b and Kidney
Embryonic Tissue Antibodies	>34 publications with IKKa/b and Embryonic Tissue
Brain Antibodies	>28 publications with IKKa/b and Brain