Rabbit Anti-ENO1 Polyclonal Antibody – Cat# MBS477163

Western blot analysis of ENO1 expression in SKBR3, Jurkat, MCF7, 3T3 and Hela cell lysates using ENO1 antibody at 1/3000 dilution.Predicted band size:47KDa.Observed band size:47KDa.

ENO1 Antibody – Rabbit Polyclonal

Name: ENO1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS477163
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477163

Entrez Summary: Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (alpha-enolase), enolase-2 (gamma-enolase) and enolase-3… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ENO1, Polyclonal Antibody

Full Product Name

Anti-ENO1 Rabbit pAb

Product Gene Name

anti-ENO1 antibody

Product Synonym Names

ENO1; ENO1L1; MBP-1; MPB1; NNE; PPH

Clonality

Polyclonal

Isotype

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Sensitivity

ENO1 Antibody detects endogenous levels of total ENO1

Form/Format

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4 degree C short term. Store at -20 degree C long term. Avoid freeze / thaw cycle.

Purity/Purification

Affinity-chromatography

Molecular Weight

47kDa

OMIM

M14328 mRNA

3D Structure

ModBase 3D Structure for P06733

Immunogen

A synthesized peptide derived from human ENO1

Applications Tested/Suitable for anti-ENO1 antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)

Application Notes for anti-ENO1 antibody

WB: 1:500~1:2000
ICC: 1:50~1:200
IP: 1:50
IHC: 1:50~1:200

Other Notes

Small volumes of anti-ENO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO1 purchase

MBS477163 is an antibody targeting ENO1. Also known as Anti-ENO1 Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO1. It may not necessarily be applicable to this product.

NCBI GI #

319996655

NCBI GeneID

2023

NCBI Accession #

NP_001188412.1

UniProt Primary Accession #

P06733

UniProt Secondary Accession #

P22712; Q16704; Q4TUS4; Q53FT9; Q53HR3; Q658M5; Q6GMP2; Q71V37; Q7Z3V6; Q8WU71; B2RD59

UniProt Related Accession #

P06733

GenBank Nucleotide #

NM_001201483.1

NCBI Official Full Name

c-myc promoter-binding protein-1 isoform MBP-1

NCBI Official Synonym Full Names

enolase 1

NCBI Official Symbol

ENO1

NCBI Official Synonym Symbols

NNE; PPH; MPB1; ENO1L1; HEL-S-17

UniProt Protein Name

Alpha-enolase

UniProt Synonym Protein Names

2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; NNE; Phosphopyruvate hydratase; Plasminogen-binding protein

Protein Family

Alpha-enolase

UniProt Gene Name

ENO1

UniProt Synonym Gene Names

ENO1L1; MBPB1; MPB1; NNE

NCBI Protein Information

alpha-enolase; c-myc promoter-binding protein-1

NCBI Summary for ENO1

This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]

UniProt Comments for ENO1

ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; extracellular space; membrane; nucleus

Molecular Function: GTPase binding; phosphopyruvate hydratase activity; protein binding

Biological Process: gluconeogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; response to virus

Research Articles on ENO1

1. High ENO1 expression is associated with cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ENO1 antibody

Products by Pathway	Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Biosynthesis Of Amino Acids Pathway antibodies	Biosynthesis Of Amino Acids Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Gluconeogenesis Pathway antibodies	Gluconeogenesis Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies	Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
Glucose Metabolism Pathway antibodies	Glucose Metabolism Pathway Diagram
Glycolysis Pathway antibodies	Glycolysis Pathway Diagram
Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies	Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram

Diseases associated with anti-ENO1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>71 publications with ENO1 and Neoplasms
Carcinoma Antibodies	>33 publications with ENO1 and Carcinoma
Nervous System Diseases Antibodies	>18 publications with ENO1 and Nervous System Diseases
Brain Diseases Antibodies	>16 publications with ENO1 and Brain Diseases
Adenocarcinoma Antibodies	>13 publications with ENO1 and Adenocarcinoma
Breast Neoplasms Antibodies	>12 publications with ENO1 and Breast Neoplasms
Arthritis, Rheumatoid Antibodies	>12 publications with ENO1 and Arthritis, Rheumatoid
Carcinoma, Non-Small-Cell Lung Antibodies	>11 publications with ENO1 and Carcinoma, Non-Small-Cell Lung
Inflammation Antibodies	>11 publications with ENO1 and Inflammation
Neoplasms, Experimental Antibodies	>9 publications with ENO1 and Neoplasms, Experimental

Organs/Tissues associated with anti-ENO1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>43 publications with ENO1 and Blood
Brain Antibodies	>28 publications with ENO1 and Brain
Lung Antibodies	>26 publications with ENO1 and Lung
Liver Antibodies	>15 publications with ENO1 and Liver
Muscle Antibodies	>13 publications with ENO1 and Muscle
Eye Antibodies	>12 publications with ENO1 and Eye
Vascular Antibodies	>11 publications with ENO1 and Vascular
Nerve Antibodies	>9 publications with ENO1 and Nerve
Heart Antibodies	>7 publications with ENO1 and Heart
Kidney Antibodies	>5 publications with ENO1 and Kidney