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PUMA Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477182

Entrez Summary: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PUMA, Polyclonal Antibody
Full Product Name
Anti-PUMA Rabbit pAb
Product Gene Name
Product Synonym Names
BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis; PUMA alpha
Clonality
Polyclonal
Isotype
Ig
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Sensitivity
PUMA Antibody detects endogenous levels of total PUMA
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4 degree C short term. Store at -20 degree C long term. Avoid freeze / thaw cycle.
Purity/Purification
Affinity-chromatography
Molecular Weight
18kDa
OMIM
AF354656 mRNA
3D Structure
ModBase 3D Structure for Q9BXH1
Immunogen
A synthesized peptide derived from human PUMA
Applications Tested/Suitable for anti-PUMA antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-PUMA antibody
WB: 1:500~1:2000
ICC: 1:50~1:200
IHC: 1:50~1:200
FC: 1:50

Western Blot (WB) of anti-PUMA antibody

Western blot analysis of PUMA expression in Hela, THP-1, K562 and HL60 cell lysates using PUMA antibody at 1/1000 dilution.Predicted band size:18KDa.Observed band size:18KDa.
Rabbit Anti-PUMA Polyclonal Antibody – Cat# MBS477182

Other Notes

Small volumes of anti-PUMA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PUMA purchase

MBS477182 is an antibody targeting PUMA. Also known as Anti-PUMA Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PUMA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
bcl-2-binding component 3 isoform 1
NCBI Official Synonym Full Names
BCL2 binding component 3
NCBI Official Symbol
BBC3
NCBI Official Synonym Symbols
JFY1; PUMA; JFY-1
UniProt Protein Name
Bcl-2-binding component 3
UniProt Synonym Protein Names
JFY-1; p53 up-regulated modulator of apoptosis
UniProt Gene Name
BBC3
UniProt Synonym Gene Names
PUMA
NCBI Protein Information
bcl-2-binding component 3

NCBI Summary for PUMA

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
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UniProt Comments for PUMA

BBC3B: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity. Induced by DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. {ECO:0000269|PubMed:11463392}. Isoform 3 does not interact with BCL2. 4 isoforms of the human protein are produced by alternative splicing

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: lysosome; mitochondrion

Molecular Function: ATPase binding; glycoprotein binding; protein binding

Biological Process: apoptosis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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