Rabbit Anti-RHO A+B+C Polyclonal Antibody – Cat# MBS477185

Western blot analysis of Rho A + B + C expression in SHSY-5Y and Rat Brain cell lysates using Rho A + B + C antibody at 1/1000 dilution.Predicted band size:22KDa.Observed band size:22KDa.

RHO A+B+C Antibody – Rabbit Polyclonal

Name: RHO A+B+C Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS477185
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477185

Entrez Summary: RhoA, RhoB, RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Rho A+B+C, Polyclonal Antibody

Full Product Name

Anti-Rho A+B+C Rabbit pAb

Product Gene Name

anti-Rho A+B+C antibody

Product Synonym Names

ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC

Clonality

Polyclonal

Isotype

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Sensitivity

Rho A + B + C Antibody detects endogenous levels of total Rho A + B + C

Form/Format

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4 degree C short term. Store at -20 degree C long term. Avoid freeze / thaw cycle.

Purity/Purification

Affinity-chromatography

Molecular Weight

22kDa

OMIM

X05026 mRNA

3D Structure

ModBase 3D Structure for P61586

Immunogen

A synthesized peptide derived from human Rho A + B + C

Applications Tested/Suitable for anti-Rho A+B+C antibody

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)

Application Notes for anti-Rho A+B+C antibody

WB: 1:500~1:2000
ICC: 1:100~1:500
IHC: 1:500~1:1000
FC: 1:200

Other Notes

Small volumes of anti-Rho A+B+C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Rho A+B+C purchase

MBS477185 is an antibody targeting Rho A+B+C. Also known as Anti-Rho A+B+C Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Rho A+B+C. It may not necessarily be applicable to this product.

NCBI GI #

927669104

NCBI GeneID

387

NCBI Accession #

NP_001300870.1

UniProt Primary Accession #

P61586

UniProt Secondary Accession #

P06749; Q53HM4; Q5U024; Q9UDJ0; Q9UEJ4

UniProt Related Accession #

P61586

GenBank Nucleotide #

NM_001313941.1

NCBI Official Full Name

transforming protein RhoA isoform 1

NCBI Official Synonym Full Names

ras homolog family member A

NCBI Official Symbol

RHOA

NCBI Official Synonym Symbols

ARHA; ARH12; RHO12; RHOH12

UniProt Protein Name

Transforming protein RhoA

UniProt Synonym Protein Names

Rho cDNA clone 12; h12

UniProt Gene Name

RHOA

UniProt Synonym Gene Names

ARH12; ARHA; RHO12; h12

NCBI Protein Information

transforming protein RhoA

NCBI Summary for Rho A+B+C

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

UniProt Comments for Rho A+B+C

RHOA: a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Controls the reorganization of actins into podosomes. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rho; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: apical junction complex; cell cortex; cell junction; cleavage furrow; cytosol; endoplasmic reticulum membrane; endosome; extrinsic to internal side of plasma membrane; focal adhesion; lamellipodium; plasma membrane; vesicle

Molecular Function: GTP binding; GTPase activity; myosin binding; protein binding

Biological Process: actin cytoskeleton organization and biogenesis; actin cytoskeleton reorganization; apical junction assembly; cell migration; endothelial cell migration; ephrin receptor signaling pathway; negative chemotaxis; negative regulation of axonogenesis; phosphoinositide-mediated signaling; platelet activation; positive regulation of axonogenesis; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of lipase activity; positive regulation of neuron differentiation; positive regulation of stress fiber formation; regulation of actin cytoskeleton organization and biogenesis; regulation of cell migration; regulation of osteoblast proliferation; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; stress fiber formation; substantia nigra development; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway

Research Articles on Rho A+B+C

1. Significant associations between ROCK1, ROCK2, RhoA and RhoC gene polymorphisms and systemic sclerosis were demonstrated.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Rho A+B+C antibody

Reference Product	PubMed Publications
ARHGEF12 antibody	>51 publications with Rho A+B+C and ARHGEF12
ARHGDIA antibody	>48 publications with Rho A+B+C and ARHGDIA
AKAP13 antibody	>25 publications with Rho A+B+C and AKAP13
RTKN antibody	>23 publications with Rho A+B+C and RTKN
DAAM1 antibody	>22 publications with Rho A+B+C and DAAM1
ARHGEF11 antibody	>22 publications with Rho A+B+C and ARHGEF11
PKN1 antibody	>5 publications with Rho A+B+C and PKN1
PKN2 antibody	>3 publications with Rho A+B+C and PKN2
UBC antibody	>3 publications with Rho A+B+C and UBC
ARHGAP1 antibody	>1 publications with Rho A+B+C and ARHGAP1

Pathways associated with anti-Rho A+B+C antibody

Products by Pathway	Pathway Diagram
AGE/RAGE Pathway antibodies	AGE/RAGE Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Alpha 6 Beta 4 Signaling Pathway antibodies	Alpha 6 Beta 4 Signaling Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Arf6 Downstream Pathway antibodies	Arf6 Downstream Pathway Diagram
Association Between Physico-Chemical Features And Toxicity Associated Pathways antibodies	Association Between Physico-Chemical Features And Toxicity Associated Pathways Diagram
Aurora B Signaling Pathway antibodies	Aurora B Signaling Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram
Axon Guidance Pathway antibodies	Axon Guidance Pathway Diagram

Diseases associated with anti-Rho A+B+C antibody

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>616 publications with Rho A+B+C and Cardiovascular Diseases
Carcinoma Antibodies	>465 publications with Rho A+B+C and Carcinoma
Disease Models, Animal Antibodies	>437 publications with Rho A+B+C and Disease Models, Animal
Inflammation Antibodies	>415 publications with Rho A+B+C and Inflammation
Neoplasm Invasiveness Antibodies	>402 publications with Rho A+B+C and Neoplasm Invasiveness
Hypertension Antibodies	>395 publications with Rho A+B+C and Hypertension
Neoplasm Metastasis Antibodies	>327 publications with Rho A+B+C and Neoplasm Metastasis
Breast Neoplasms Antibodies	>236 publications with Rho A+B+C and Breast Neoplasms
Heart Diseases Antibodies	>228 publications with Rho A+B+C and Heart Diseases
Fibrosis Antibodies	>221 publications with Rho A+B+C and Fibrosis

Organs/Tissues associated with anti-Rho A+B+C antibody

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>1466 publications with Rho A+B+C and Vascular
Muscle Antibodies	>1406 publications with Rho A+B+C and Muscle
Blood Antibodies	>1217 publications with Rho A+B+C and Blood
Brain Antibodies	>826 publications with Rho A+B+C and Brain
Lung Antibodies	>582 publications with Rho A+B+C and Lung
Nerve Antibodies	>528 publications with Rho A+B+C and Nerve
Heart Antibodies	>498 publications with Rho A+B+C and Heart
Kidney Antibodies	>420 publications with Rho A+B+C and Kidney
Bone Antibodies	>324 publications with Rho A+B+C and Bone
Liver Antibodies	>319 publications with Rho A+B+C and Liver