Mouse Anti-CDK4 Monoclonal Antibody – Cat# MBS477195

Western blot detection of CDK4 in Hela and K562 cell lysates using CDK4 mouse mAb (1:500 diluted).Predicted band size:34KDa.Observed band size:34KDa.

CDK4 Antibody – Mouse Monoclonal

Name: CDK4 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS477195
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477195

Entrez Summary: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

CDK4, Monoclonal Antibody

Full Product Name

Anti-CDK4 Mouse mAb

Product Gene Name

anti-CDK4 antibody

Product Synonym Gene Name

CDK4; CMM3; PSK-J3.

Product Synonym Names

Cdk4; cdk4; CDK4protein; CDK4_HUMAN; Celldivisionkinase4; Celldivisionproteinkinase4; CMM3; CMM3; Crk3; Cyclindependentkinase4; Cyclin-dependentkinase4; Melanomacutaneousmalignant3; MGC14458; p34cdk4; PSKJ3; PSK-J3

Clonality

Monoclonal

Isotype

lgG1

Clone Number

3D12-A7-F11

Host

Mouse

Species Reactivity

Human

Sensitivity

CDK4 Mouse mAb recognizes endogenous levels of total CDK4 protein.

Form/Format

Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Purity/Purification

Affinity Purified

Concentration

3.86mg/ml (lot specific)

Molecular Weight

34kDa

OMIM

M14505 mRNA

3D Structure

ModBase 3D Structure for P11802

Immunogen

Purified recombinant human CDK4 protein expressed in E Coli.

Sequence Similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Subunit Structure

Component of the D-CDK4 complex, composed of CDK4 and some D-type G1 cyclin (CCND1, CCND2 or CCND3). Interacts directly in the complex with CCND1, CCND2 or CCND3. Interacts with SEI1 and ZNF655. Forms a ternary complex, cyclin D-CDK4-CDKN1B, involved in modulating CDK4 enzymatic activity. Interacts directly with CDKN1B (phosphorylated on 'Tyr-88' and 'Tyr-89'); the interaction allows assembly of the cyclin D-CDK4 complex, Thr-172 phosphorylation, nuclear translocation and enhances the cyclin D-CDK4 complex activity. CDK4 activity is either inhibited or enhanced depending on stoichiometry of complex. The non-tyrosine-phosphorylated form of CDKN1B prevents T-loop phosphorylation of CDK4 producing inactive CDK4. Interacts (unphosphorylated form) with CDK2. Also forms ternary complexes with CDKN1A or CDKN2A. Interacts directly with CDKN1A (via its N-terminal); the interaction promotes the assembly of the cyclin D-CDK4 complex, its nuclear translocation and promotes the cyclin D-dependent enzyme activity of CDK4. Interacts with CCND1; the interaction is prevented with the binding of CCND1 to INSM1 during cell cycle progression. Interacts with CEBPA (when phosphorylated).

Subcellular Location

Cytoplasm ; Nucleus ; Membrane

Applications Tested/Suitable for anti-CDK4 antibody

Western Blot (WB)

Application Notes for anti-CDK4 antibody

WB: 1:500

Other Notes

Small volumes of anti-CDK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDK4 purchase

MBS477195 is an antibody targeting CDK4. Also known as Anti-CDK4 Mouse mAb. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CDK4. It may not necessarily be applicable to this product.

NCBI GI #

4502735

NCBI GeneID

1019

NCBI Accession #

NP_000066.1

UniProt Primary Accession #

P11802

UniProt Secondary Accession #

O00576; Q6FG61; B2R9A0; B4DNF9

UniProt Related Accession #

P11802

GenBank Nucleotide #

NM_000075.2

NCBI Official Full Name

cyclin-dependent kinase 4

NCBI Official Synonym Full Names

cyclin dependent kinase 4

NCBI Official Symbol

CDK4

NCBI Official Synonym Symbols

CMM3; PSK-J3

UniProt Protein Name

Cyclin-dependent kinase 4

UniProt Synonym Protein Names

Cell division protein kinase 4; PSK-J3

Protein Family

Cyclin-dependent kinase

UniProt Gene Name

CDK4

NCBI Protein Information

cyclin-dependent kinase 4

NCBI Summary for CDK4

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

UniProt Comments for CDK4

CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991.

Protein type: CDK family; CDK/CDK4 subfamily; CDK4 subfamily; CMGC group; Cell cycle regulation; EC 2.7.11.22; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 12q14.1

Cellular Component: chromatin; cyclin-dependent protein kinase holoenzyme complex; cytosol; nuclear membrane; nucleolus; nucleoplasm; nucleus

Molecular Function: cyclin binding; cyclin-dependent protein kinase activity; cyclin-dependent protein kinase regulator activity; protein binding

Biological Process: G1/S transition of mitotic cell cycle; positive regulation of cell cycle; positive regulation of cell proliferation; positive regulation of fibroblast proliferation; protein amino acid phosphorylation; regulation of gene expression; response to drug

Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3

Research Articles on CDK4

1. MiR-16 upregulation could reduce CDK4 expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDK4 antibody

Reference Product	PubMed Publications
CCND1 antibody	>1837 publications with CDK4 and CCND1
CDKN2A antibody	>953 publications with CDK4 and CDKN2A
CDKN1A antibody	>724 publications with CDK4 and CDKN1A
CDKN1B antibody	>649 publications with CDK4 and CDKN1B
CCND3 antibody	>147 publications with CDK4 and CCND3
CCND2 antibody	>140 publications with CDK4 and CCND2
RB1 antibody	>67 publications with CDK4 and RB1
CDC37 antibody	>28 publications with CDK4 and CDC37
CCNA2 antibody	>25 publications with CDK4 and CCNA2
CCNE1 antibody	>25 publications with CDK4 and CCNE1

Pathways associated with anti-CDK4 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Bladder Cancer Pathway antibodies	Bladder Cancer Pathway Diagram
Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies	Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram

Diseases associated with anti-CDK4 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2279 publications with CDK4 and Neoplasms
Carcinoma Antibodies	>701 publications with CDK4 and Carcinoma
Breast Neoplasms Antibodies	>270 publications with CDK4 and Breast Neoplasms
Adenocarcinoma Antibodies	>265 publications with CDK4 and Adenocarcinoma
Cell Transformation, Neoplastic Antibodies	>225 publications with CDK4 and Cell Transformation, Neoplastic
Nervous System Diseases Antibodies	>207 publications with CDK4 and Nervous System Diseases
Neoplasms, Experimental Antibodies	>200 publications with CDK4 and Neoplasms, Experimental
Skin Neoplasms Antibodies	>196 publications with CDK4 and Skin Neoplasms
Lung Neoplasms Antibodies	>151 publications with CDK4 and Lung Neoplasms
Liver Diseases Antibodies	>147 publications with CDK4 and Liver Diseases

Organs/Tissues associated with anti-CDK4 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>381 publications with CDK4 and Blood
Skin Antibodies	>259 publications with CDK4 and Skin
Lung Antibodies	>251 publications with CDK4 and Lung
Muscle Antibodies	>251 publications with CDK4 and Muscle
Liver Antibodies	>245 publications with CDK4 and Liver
Brain Antibodies	>238 publications with CDK4 and Brain
Vascular Antibodies	>196 publications with CDK4 and Vascular
Bone Antibodies	>149 publications with CDK4 and Bone
Prostate Antibodies	>143 publications with CDK4 and Prostate
Kidney Antibodies	>97 publications with CDK4 and Kidney