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Enolase-2 (ENO2) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477201

Entrez Summary: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during… More details

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Product Overview

Online Datasheet in PDF
Product Name
Enolase-2 (ENO2), Monoclonal Antibody
Full Product Name
Anti-Enolase-2 Mouse mAb
Product Gene Name
Product Synonym Gene Name
ENO2; HEL-S-279; NSE
Product Synonym Names
2 phospho D glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; Eno 2; ENO2; ENOG; ENOG_HUMAN; Enolase 2 (gamma, neuronal); Enolase 2; Enolase 2 gamma neuronal; Enolase2; Epididymis secretory protein Li 279; Gamma enolase; Gamma-enolase; HEL S 279; Neural enolase; Neuron specific enolase; Neuron specific gamma enolase; Neuron-specific enolase; Neurone specific enolase; NSE
Clonality
Monoclonal
Isotype
lgG1
Clone Number
1H8-B4
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Sensitivity
This antibody detects endogenous levels of Enolase-2 and does not cross-react with Enolase-1 or other related proteins.
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300.
Purity/Purification
Affinity Purified
Concentration
4.5mg/ml (lot specific)
Molecular Weight
47kDa
OMIM
X13120 mRNA
3D Structure
ModBase 3D Structure for P09104
Immunogen
Synthetic peptide derived from human NSE.
Sequence Similarities
Belongs to the enolase family.
Subunit Structure
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.
Subcellular Location
Cytoplasm; Cell membrane
Applications Tested/Suitable for anti-ENO2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-ENO2 antibody
WB: 1:500

Western Blot (WB) of anti-ENO2 antibody

Western blot detection of Enolase-2 in SHSY-5Y and U87-MG cell lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.
Mouse Anti-ENO2 Monoclonal Antibody – Cat# MBS477201

Western Blot (WB) of anti-ENO2 antibody

Western blot detection of Enolase-2 in Rat Brain and Mouse Brain lysates using Enolase-2 mouse mAb (1:500 diluted).Predicted band size:47KDa.Observed band size:47KDa.
Mouse Anti-ENO2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS477201

Testing Data of anti-ENO2 antibody

Observed Enolase-2 levels in CHO-K1 cell lysates transfected with Enolase-2 at different dilution.
Mouse Anti-ENO2 Monoclonal Antibody, Testing Data – Cat# MBS477201

Other Notes

Small volumes of anti-ENO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ENO2 purchase

MBS477201 is an antibody targeting ENO2. Also known as Anti-Enolase-2 Mouse mAb. Reactive in Human, Mouse, Rat. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ENO2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2
NCBI Official Symbol
ENO2
NCBI Official Synonym Symbols
NSE; HEL-S-279
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase; NSE
UniProt Gene Name
ENO2
UniProt Synonym Gene Names
NSE
NCBI Protein Information
gamma-enolase

NCBI Summary for ENO2

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]

UniProt Comments for ENO2

ENO2: an enzyme with 2-phospho-D-glycerate hydro-lyase activity. One of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: cytosol; extracellular space

Molecular Function: phosphopyruvate hydratase activity; protein binding

Biological Process: gluconeogenesis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ENO2 antibody

 Reference Product  PubMed Publications
 HK1 antibody  >2 publications with ENO2 and HK1
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