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Tri-Methyl-Histone H3 (HIST1H3A) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477240

MBS477240 is an antibody targeting HIST1H3A. Also known as Anti-Tri-Methyl-Histone H3 (Lys79) Mouse mAb. Reactive in Human, Rat, Monkey. Host: Mouse. More details

$525 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Tri-Methyl-Histone H3 (HIST1H3A), Monoclonal Antibody
Full Product Name
Anti-Tri-Methyl-Histone H3 (Lys79) Mouse mAb
Product Gene Name
anti-HIST1H3A antibody
Product Synonym Gene Name
H3/A; H3FA
Product Synonym Names
HIST1 cluster; H3J; Histone gene cluster 1; H3 histone family; member E; Histone gene cluster 1; H3G; H3 histone family; member J; HIST1 cluster; H3E; HIST1 cluster; H3I; Histone gene cluster 1; H3C; FLJ92264; H3 histone family; member A; H3 histone family; member B; H3 histone family; member C; H3 histone family; member D; H3 histone family; member F; H3 histone family; member H; H3 histone family; member I; H3 histone family; member K; H3 histone family; member L; H3 histone family; member T; H3 histone; family 3A; H3.1; H3.3A; H3/a; H3/b; H3/c; H3/d; h3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H3/t; H31_HUMAN; H3F1K; H3F3; H3F3A; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1 cluster; H3A; HIST1 cluster; H3B; HIST1 cluster; H3C; HIST1 cluster; H3D; HIST1 cluster; H3F; HIST1 cluster; H3G; HIST1 cluster; H3H; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; Histone 1; H3a; Histone 1; H3b; Histone 1; H3c; Histone 1; H3d; Histone 1; H3e; Histone 1; H3f; Histone 1; H3g; Histone 1; H3h; Histone 1; H3i; Histone 1; H3j; histone 3; H3; histone cluster 1 H3 family member a; histone cluster 1 H3 family member b; histone cluster 1 H3 family member c; histone cluster 1 H3 family member d; histone cluster 1 H3 family member e; histone cluster 1 H3 family member f; histone cluster 1 H3 family member g; histone cluster 1 H3 family member h; histone cluster 1 H3 family member i; histone cluster 1 H3 family member j; Histone cluster 1; H3a; Histone cluster 1; H3b; Histone cluster 1; H3c; Histone cluster 1; H3d; Histone cluster 1; H3e; Histone cluster 1; H3f; Histone cluster 1; H3g; Histone cluster 1; H3i; Histone cluster 1; H3j; Histone gene cluster 1; H3 histone family; member A; Histone gene cluster 1; H3 histone family; member B; Histone gene cluster 1; H3 histone family; member C; Histone gene cluster 1; H3 histone family; member D; Histone gene cluster 1; H3 histone family; member F; Histone gene cluster 1; H3 histone family; member G; Histone gene cluster 1; H3 histone family; member H; Histone gene cluster 1; H3 histone family; member I; Histone gene cluster 1; H3 histone family; member J; Histone gene cluster 1; H3A; Histone gene cluster 1; H3B; Histone gene cluster 1; H3D; Histone gene cluster 1; H3E; Histone gene cluster 1; H3F; Histone gene cluster 1; H3H; Histone gene cluster 1; H3I; Histone gene cluster 1; H3J; Histone H 3; Histone H3.1; histone H3.1t; Histone H3.2; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; Histone H3/m; Histone H3/o
Clonality
Monoclonal
Host
Mouse
Species Reactivity
Human, Rat, Monkey
Form/Format
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Purity/Purification
Fractional precipitation
Molecular Weight
17kDa
OMIM
137800
3D Structure
ModBase 3D Structure for P68431
Immunogen
Synthetic Peptide of Histone H3 (Tri Methyl Lys79).
Subcellular Location
Chromosome
Applications Tested/Suitable for anti-HIST1H3A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-HIST1H3A antibody
WB: 1:500-2,000

Western Blot (WB) of anti-HIST1H3A antibody

Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 with Histone H3 (tri methyl K79) (3G3) Mouse MAb diluted at 1:2000.
Mouse Anti-HIST1H3A Monoclonal Antibody – Cat# MBS477240

Testing Data of anti-HIST1H3A antibody

~Input: Hela Cell Lysate -IP product: IP dilute 1:200 Western blot analysis: primary antibody : 1:1, 000 Secondary antibody: Goat anti-Mouse IgG(H+L) (S001) 1:10, 000
Mouse Anti-HIST1H3A Monoclonal Antibody, Testing Data – Cat# MBS477240

Other Notes

Small volumes of anti-HIST1H3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HIST1H3A. It may not necessarily be applicable to this product.

NCBI GI #
4504281
NCBI GeneID
8350
NCBI Accession #
NP_003520.1
UniProt Primary Accession #
P68431
UniProt Secondary Accession #
P02295; P02296; P16106; Q6ISV8; Q6NWP8; Q6NWP9; Q6NXU4; Q71DJ3; Q93081; A0PJT7; A5PLR1
UniProt Related Accession #
P68431
GenBank Nucleotide #
NM_003529.2
NCBI Official Full Name
histone H3.1
NCBI Official Synonym Full Names
histone cluster 1 H3 family member a
NCBI Official Symbol
HIST1H3A
NCBI Official Synonym Symbols
H3/A; H3FA
UniProt Protein Name
Histone H3.1
UniProt Synonym Protein Names
Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l
UniProt Gene Name
HIST1H3A
UniProt Synonym Gene Names
H3FA
NCBI Protein Information
histone H3.1

NCBI Summary for HIST1H3A

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
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UniProt Comments for HIST1H3A

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).CautionThe original paper reporting lysine deamination at Lys-5 by LOXL2 has been retracted due to inappropriate manipulation of figure data (PubMed:22483618, PubMed:27392148). However, this modification was confirmed in a subsequent publication (PubMed:27735137).
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Research Articles on HIST1H3A

1. These results identify cytokine-induced histone 3 lysine 27 trimethylation as a mechanism that stabilizes gene silencing in macrophages

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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