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PI3 Kinase P85alpha (PIK3R1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477653

MBS477653 is an antibody targeting PIK3R1. Also known as Anti-PI3 Kinase P85alpha Rabbit pAb. Reactive in Human, Rat, Mouse. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
PI3 Kinase P85alpha (PIK3R1), Polyclonal Antibody
Full Product Name
Anti-PI3 Kinase P85alpha Rabbit pAb
Product Gene Name
Product Synonym Names
GRB1 antibody; p85 alpha antibody; PI3K antibody; PIK3R1 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Form/Format
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Purity/Purification
Affinity Purified
Preparation and Storage
Store at -20 degree C.
Molecular Weight
85
OMIM
171833
3D Structure
ModBase 3D Structure for P27986
Immunogen
Recombinant Protein
Applications Tested/Suitable for anti-PIK3R1 antibody
Western Blot (WB)
Application Notes for anti-PIK3R1 antibody
WB: 1:1,000-2,000

Western Blot (WB) of anti-PIK3R1 antibody

Western blot analysis of 1) HepG2, 2) 293T, with PI3 Kinase p85u03b1 Rabbit pAb diluted at 1:2, 000.
Rabbit Anti-PIK3R1 Polyclonal Antibody – Cat# MBS477653

Other Notes

Small volumes of anti-PIK3R1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PIK3R1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
phosphatidylinositol 3-kinase regulatory subunit alpha isoform 4
NCBI Official Synonym Full Names
phosphoinositide-3-kinase regulatory subunit 1
NCBI Official Symbol
PIK3R1
NCBI Official Synonym Symbols
p85; AGM7; GRB1; IMD36; p85-ALPHA
UniProt Protein Name
Phosphatidylinositol 3-kinase regulatory subunit alpha
UniProt Synonym Protein Names
Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
UniProt Gene Name
PIK3R1
UniProt Synonym Gene Names
GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
NCBI Protein Information
phosphatidylinositol 3-kinase regulatory subunit alpha

NCBI Summary for PIK3R1

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
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UniProt Comments for PIK3R1

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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