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HIF-2 alpha Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477808

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and… More details

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Product Overview

Product Name
HIF-2 alpha, Polyclonal Antibody
Full Product Name
Anti-HIF-2 alpha Rabbit pAb
Product Gene Name
Product Synonym Gene Name
bHLHe73; ECYT4; EPAS 1; EPAS1; HIF2A; HLF; MOP 2; PASD2
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Chromatography
Specificity
HIF-2-alpha Antibody detects endogenous levels of total HIF-2-alpha
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Molecular Weight
96kDa
OMIM
603349
3D Structure
ModBase 3D Structure for Q99814
Immunogen
A synthesized peptide derived from human HIF-2 alpha
Subcellular Location
Nucleus speckle
Applications Tested/Suitable for anti-HIF-2 alpha antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-HIF-2 alpha antibody
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-HIF-2 alpha antibody

Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate; (2)HeLa cell lysate.
Rabbit Anti-HIF-2 alpha Polyclonal Antibody – Cat# MBS477808

Other Notes

Small volumes of anti-HIF-2 alpha antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HIF-2 alpha purchase

MBS477808 is an antibody targeting HIF-2 alpha. Also known as Anti-HIF-2 alpha Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for HIF-2 alpha. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
endothelial PAS domain-containing protein 1
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
EPAS1
NCBI Official Synonym Symbols
HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Synonym Protein Names
Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73; HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
UniProt Gene Name
EPAS1
UniProt Synonym Gene Names
BHLHE73; HIF2A; MOP2; PASD2; EPAS-1; bHLHe73; HLF; HIF-2-alpha; HIF2-alpha
NCBI Protein Information
endothelial PAS domain-containing protein 1

NCBI Summary for HIF-2 alpha

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]
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UniProt Comments for HIF-2 alpha

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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