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SMAD5 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477825

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Smad5, Polyclonal Antibody
Full Product Name
Anti-Phospho-Smad5 (S463/465) Rabbit pAb
Product Gene Name
Product Synonym Names
hSmad5; JV5-1; MADH5; SMAD5; MusMLP; SMAD family member 5
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
Affinity Chromatography
Specificity
Phospho-Smad5 (S463/465) Antibody detects endogenous levels of total Phospho-Smad5 (S463/465)
Preparation and Storage
Store at 4 degree C for short term.
Store at-20 degree C for long term.
Avoid freeze/thaw cycle.
Molecular Weight
58kDa
OMIM
603110
3D Structure
ModBase 3D Structure for Q99717
Immunogen
A synthesized peptide derived from human Phospho-Smad5 (S463/465)
Applications Tested/Suitable for anti-Smad5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-Smad5 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200

Western Blot (WB) of anti-Smad5 antibody

Western blot analysis of Phospho-Smad5 in (1)Mouse brain tissue lysate; (2)Rat brain tissue lysate.
Rabbit Anti-SMAD5 Polyclonal Antibody – Cat# MBS477825

Immunohistochemistry (IHC) of anti-Smad5 antibody

Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-Smad5 (S463/465) Antibody.
Rabbit Anti-SMAD5 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS477825

Other Notes

Small volumes of anti-Smad5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Smad5 purchase

MBS477825 is an antibody targeting Smad5. Also known as Anti-Phospho-Smad5 (S463/465) Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Smad5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
mothers against decapentaplegic homolog 5
NCBI Official Synonym Full Names
SMAD family member 5
NCBI Official Symbol
SMAD5
NCBI Official Synonym Symbols
DWFC; JV5-1; MADH5
UniProt Protein Name
Mothers against decapentaplegic homolog 5
UniProt Synonym Protein Names
JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5
UniProt Gene Name
SMAD5
UniProt Synonym Gene Names
MADH5; MAD homolog 5; Mothers against DPP homolog 5; SMAD 5; Smad5; hSmad5
NCBI Protein Information
mothers against decapentaplegic homolog 5

NCBI Summary for Smad5

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
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UniProt Comments for Smad5

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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