GST T2-2 Recombinant Protein

Rat recombinant protein expressed in E Coli.

>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.

Specific Activity: Units/mg using spectrophotometric determination of 4- nitrobenzyl chloride (NBC) conjugation with reduced glutathione (5 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.

At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES.

23 kDa

50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 5 mM EDTA and 50% glycerol.

This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity toward CDNB and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than CDNB.