GST A1-1 Recombinant Protein

Name: GST A1-1 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS480645
Price: 695 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS480645

MBS480645 is a recombinant protein targeting GST A1-1. Also known as GST A1-1, Recombinant Bovine. Host: Bovine recombinant protein expressed in E Coli. More details

$695 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GST A1-1, Recombinant Protein

Full Product Name

GST A1-1, Recombinant Bovine

Product Gene Name

GST A1-1 recombinant protein

Product Synonym Names

Recombinant Bovine GST A1-1

Host

Bovine recombinant protein expressed in E Coli

Purity/Purification

>= 95% as assessed by inspection on a Coomassie Blue-stained SDS-PAGE gel.

Concentration

1.42 mg/mL total protein using the Bradford protein assay with BSA as a standard (lot specific)

Specificity

Specific Activity: 4.35 Units/mg using spectrophotometric determination of 1- chloro-2,4-dinitrobenzene (CDNB) conjugation with reduced glutathione (1 mM) in 100 mM NaPO4 (pH 6.5) at room temperature.

Preparation and Storage

At -20 degree C; AVOID MULTIPLE FREEZE-THAW CYCLES

Molecular Weight

23 kDa

3D Structure

ModBase 3D Structure for P00502

Storage Buffer

50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT, 1 mM EDTA and 50% glycerol.

Procedural Notes

This purified product exhibits high enzymatic activity for CDNB, the synthetic substrate that is most commonly used for GST analyses. However, as is widely known for GST activity analysis using CDNB as a substrate, low concentrations (high dilutions) of the enzyme result in lower activity values. In contrast, initial velocities are much higher for more concentrated levels of enzyme but the rate decreases rapidly. Therefore, activity toward CDNB and these considerations provide guidance when assaying this product under low concentrations or when using substrates other than CDNB.

Product References and Citations for GST A1-1 recombinant protein

Raffalli-Mathieu, F., et. al; (2007) Biochimica et Biophysica Acta 1770:130-136

Other Notes

Small volumes of GST A1-1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GST A1-1. It may not necessarily be applicable to this product.

NCBI GI #

51036637

NCBI GeneID

24422

NCBI Accession #

NP_058709.2

UniProt Primary Accession #

P00502

UniProt Secondary Accession #

Q6AZ72

UniProt Related Accession #

P00502

GenBank Nucleotide #

NM_017013.4

NCBI Official Full Name

glutathione S-transferase alpha-1

NCBI Official Synonym Full Names

glutathione S-transferase alpha 2

NCBI Official Symbol

Gsta2

NCBI Official Synonym Symbols

Gsta1

UniProt Protein Name

Glutathione S-transferase alpha-1

UniProt Synonym Protein Names

GST 1-1; GST 1a-1a; GST A1-1; GST B; Glutathione S-transferase Ya-1; GST Ya1

Protein Family

Glutathione S-transferase

UniProt Gene Name

Gsta1

UniProt Synonym Gene Names

GST Ya1

UniProt Entry Name

GSTA1_RAT

NCBI Protein Information

glutathione S-transferase alpha-1; GST 1-1; GST 1a-1a; GST A1-1; GST B; GST Ya1; Glutathione-S-transferase alpha type (Yc); glutathione S-transferase A2; glutathione S-transferase Ya subunit; glutathione S-transferase Ya-1; glutathione-S-transferase, alpha type2; ligandin

NCBI Summary for GST A1-1

mRNA expression is induced by phenobarbital and by trans-stilbene oxide [RGD, Feb 2006]

UniProt Comments for GST A1-1

GSTA5: Belongs to the GST superfamily. Alpha family. Homodimer

Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Transferase; Xenobiotic Metabolism - metabolism by cytochrome P450; Nuclear envelope

Cellular Component: nuclear outer membrane; cytosol; nucleus

Molecular Function: glutathione transferase activity; glutathione binding; drug binding

Biological Process: metabolic process; xenobiotic catabolic process; aging

Research Articles on GST A1-1

1. The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GST A1-1 recombinant protein

Reference Product	PubMed Publications
Cyp1a1 recombinant protein	>15 publications with GST A1-1 and Cyp1a1
Cyp1a2 recombinant protein	>11 publications with GST A1-1 and Cyp1a2
Cyp2e1 recombinant protein	>6 publications with GST A1-1 and Cyp2e1
Gstt2 recombinant protein	>6 publications with GST A1-1 and Gstt2
Gstt1 recombinant protein	>6 publications with GST A1-1 and Gstt1
Cyp2b1 recombinant protein	>4 publications with GST A1-1 and Cyp2b1
Mgst3 recombinant protein	>3 publications with GST A1-1 and Mgst3
Mgst1 recombinant protein	>2 publications with GST A1-1 and Mgst1
Aldh3a1 recombinant protein	>1 publications with GST A1-1 and Aldh3a1

Pathways associated with GST A1-1 recombinant protein

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Chemical Carcinogenesis Pathway antibodies	Chemical Carcinogenesis Pathway Diagram
Chemical Carcinogenesis Pathway antibodies	Chemical Carcinogenesis Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Drug Metabolism - Cytochrome P450 Pathway antibodies	Drug Metabolism - Cytochrome P450 Pathway Diagram
Glutathione Conjugation Pathway antibodies	Glutathione Conjugation Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Keap1-Nrf2 Pathway antibodies	Keap1-Nrf2 Pathway Diagram

Diseases associated with GST A1-1 recombinant protein

Disease Name	Pubmed Publications
Liver Diseases Antibodies	>18 publications with GST A1-1 and Liver Diseases
Liver Neoplasms Antibodies	>9 publications with GST A1-1 and Liver Neoplasms
Inflammation Antibodies	>8 publications with GST A1-1 and Inflammation
Disease Models, Animal Antibodies	>4 publications with GST A1-1 and Disease Models, Animal
Neoplasms, Experimental Antibodies	>4 publications with GST A1-1 and Neoplasms, Experimental
Drug-Related Side Effects and Adverse Reactions Antibodies	>4 publications with GST A1-1 and Drug-Related Side Effects and Adverse Reactions
Poisoning Antibodies	>4 publications with GST A1-1 and Poisoning
Drug-Induced Liver Injury Antibodies	>4 publications with GST A1-1 and Drug-Induced Liver Injury
Carcinoma, Hepatocellular Antibodies	>3 publications with GST A1-1 and Carcinoma, Hepatocellular
Liver Neoplasms, Experimental Antibodies	>3 publications with GST A1-1 and Liver Neoplasms, Experimental

Organs/Tissues associated with GST A1-1 recombinant protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>58 publications with GST A1-1 and Liver
Kidney Antibodies	>5 publications with GST A1-1 and Kidney
Ovary Antibodies	>2 publications with GST A1-1 and Ovary
Small Intestine Antibodies	>2 publications with GST A1-1 and Small Intestine
Heart Antibodies	>1 publications with GST A1-1 and Heart