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IL-16 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488510

Describes the original generation of this antibody and its use in molecular cloning of IL-16.Recombinant monoclonal antibody to IL-16. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 14.1. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
IL-16, Monoclonal Antibody
Full Product Name
Anti-IL-16 [14.1], Mouse IgG2a, Kappa
Product Gene Name
Product Synonym Names
LCF; interleukin 16; NPRprIL-16; IL16
Clonality
Monoclonal
Isotype
Mouse IgG2a, Kappa
Clone Number
14.1
Host
Mouse
Species Reactivity
Human
Form/Format
PBS with 0.02% Proclin 300
Purity/Purification
Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
The antibody is specific for IL-16.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
141,752 Da
Chromosome Location
Chromosome: 15; NC_000015.9 (81489219..81605104). Location: 15q26.3
OMIM
603035
3D Structure
ModBase 3D Structure for Q14005
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Antibody Type
Recombinant Antibody
Immunogen
The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-IL-16 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes for anti-IL-16 antibody
The antibody has been used in Western blot analysis to detect IL-16. It has been used in ELISA-type binding assays. The antibody has been used to stain endogenous IL-16 in sheets from murine ear skin after intradermal injection of TNF-alpha, and in flow cytometry to detect IL-16 on epidermal cells and emigrated cutaneous DC (Stoitzner et al, 2001).

Flow Cytometry (FC/FACS) of anti-IL-16 antibody

Flow-cytometry using the Anti-IL-16 antibody 14.1 or the rabbit IgG version of 14.1 at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.
Mouse Anti-IL-16 Monoclonal Antibody – Cat# MBS488510

Product References and Citations for anti-IL-16 antibody

Cruikshank et al.; Proc Natl Acad Sci USA. 1994 May 24;91(11):5109-13. OriginPubPMID: 27231345

Other Notes

Small volumes of anti-IL-16 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL-16 purchase

MBS488510 is an antibody targeting IL-16. Also known as Anti-IL-16 [14.1], Mouse IgG2a, Kappa. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IL-16. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
IL16 protein
NCBI Official Synonym Full Names
interleukin 16
NCBI Official Symbol
IL16
NCBI Official Synonym Symbols
LCF; NIL16; PRIL16; prIL-16
UniProt Protein Name
Pro-interleukin-16
UniProt Synonym Protein Names
Lymphocyte chemoattractant factor
UniProt Gene Name
IL16
UniProt Synonym Gene Names
IL-16; LCF
UniProt Entry Name
IL16_HUMAN
NCBI Protein Information
pro-interleukin-16; prointerleukin 16; neuronal interleukin 16; lymphocyte chemoattractant factor

NCBI Summary for IL-16

The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
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UniProt Comments for IL-16

IL16: Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4. 3 isoforms of the human protein are produced by alternative promoter.

Protein type: Cytokine; DNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: extracellular space; cytoplasm; plasma membrane; nucleus

Molecular Function: cytokine activity

Biological Process: viral reproduction; regulation of transcription, DNA-dependent; transcription, DNA-dependent; leukocyte chemotaxis; immune response; induction of positive chemotaxis
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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