Mouse Anti-DR5 Monoclonal Antibody – Cat# MBS488513

Immunofluorescence staining of HepG2 cells using anti-DR5 antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

DR5 Antibody – Mouse Monoclonal

Name: DR5 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS488513
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488513

Describes the generation of the antibody, its characterisation and its function in triggering tumor apoptosis.Recombinant monoclonal antibody to DR5. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma TRA-8. More details

$635 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DR5, Monoclonal Antibody

Full Product Name

Anti-DR5 [TRA-8], Rabbit IgG, Kappa

Product Gene Name

anti-DR5 antibody

Product Synonym Names

TRAILR2; TNFRSF10B; TRAIL receptor 2; tumor necrosis factor receptor superfamily member 10B

Clonality

Monoclonal

Isotype

Rabbit IgG, Kappa

Clone Number

TRA-8

Host

Mouse

Species Reactivity

Human

Form/Format

PBS with 0.02% Proclin 300

Purity/Purification

Protein A affinity purified

Concentration

1mg/ml (lot specific)

Specificity

The antibody is specific to human DR5 and does not bind other TRAIL receptors, other members of the TNF-receptor superfamily, or the murine homolog of DR5.

Preparation and Storage

Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Molecular Weight

47,878 Da

OMIM

275355

3D Structure

ModBase 3D Structure for O14763

Animal-free Manufacturing Platform

The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.

Antibody Type

Recombinant Antibody

Immunogen

The antibody was raised by immunizing BALB/c mice with a fusion protein containing the extracellular portion of human DR5 and the Fc portion of human IgG1.

Chimeric Use Statement

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for anti-DR5 antibody

Oncology; Apoptosis; Cell signalling

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-DR5 antibody

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

Application Notes for anti-DR5 antibody

The antibody has been characterised using Western blot and ELISA. It induces apoptosis in primary hepatocellular carcinoma cells and established liver cancer cell lines, but not normal human hepatocytes (Ichikawa et al., 2001). The antibody has been reported to function in flow cytometry to detect cell-surface expression of DR5 on Cos-7 cells transfected with full-length human DR5.

Product References and Citations for anti-DR5 antibody

Ichikawa et al.; Nat Med. 2001 Aug;7(8):954-60.

Other Notes

Small volumes of anti-DR5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DR5 purchase

MBS488513 is an antibody targeting DR5. Also known as Anti-DR5 [TRA-8], Rabbit IgG, Kappa. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for DR5. It may not necessarily be applicable to this product.

NCBI GI #

224494019

NCBI GeneID

8795

NCBI Accession #

NP_003833.4

UniProt Primary Accession #

O14763

UniProt Secondary Accession #

O14720; O15508; O15517; O15531; Q6UXM8; Q7Z360; Q9BVE0

UniProt Related Accession #

O14763

GenBank Nucleotide #

NM_003842.4

NCBI Official Full Name

tumor necrosis factor receptor superfamily member 10B isoform 1

NCBI Official Synonym Full Names

tumor necrosis factor receptor superfamily, member 10b

NCBI Official Symbol

TNFRSF10B

NCBI Official Synonym Symbols

DR5; CD262; KILLER; TRICK2; TRICKB; ZTNFR9; TRAILR2; TRICK2A; TRICK2B; TRAIL-R2; KILLER/DR5

UniProt Protein Name

Tumor necrosis factor receptor superfamily member 10B

UniProt Synonym Protein Names

Death receptor 5; TNF-related apoptosis-inducing ligand receptor 2

Protein Family

Transposon Ty1-DR5 Gag-Pol polyprotein

UniProt Gene Name

TNFRSF10B

UniProt Synonym Gene Names

DR5; KILLER; TRAILR2; TRICK2; ZTNFR9; TRAIL receptor 2; TRAIL-R2

UniProt Entry Name

TR10B_HUMAN

NCBI Protein Information

tumor necrosis factor receptor superfamily member 10B; Fas-like protein; death receptor 5; cytotoxic TRAIL receptor-2; TNF receptor superfamily member 10b; apoptosis inducing receptor TRAIL-R2; apoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-

NCBI Summary for DR5

The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]

UniProt Comments for DR5

TRAIL-R2: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Homotrimer. Can interact with TRADD and RIPK1. Regulated by p53/TP53. Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p22-p21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; TRAIL binding; receptor activity

Biological Process: regulation of apoptosis; caspase activation; cell surface receptor linked signal transduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; apoptosis; activation of NF-kappaB-inducing kinase

Disease: Squamous Cell Carcinoma, Head And Neck

Research Articles on DR5

1. DR5 integrates opposing UPR signals to couple the endoplasmic reticulum stress and apoptotic cell fate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DR5 antibody

Reference Product	PubMed Publications
TNFSF10 antibody	>2722 publications with DR5 and TNFSF10
CASP8 antibody	>782 publications with DR5 and CASP8
FADD antibody	>413 publications with DR5 and FADD
CFLAR antibody	>156 publications with DR5 and CFLAR
TRADD antibody	>48 publications with DR5 and TRADD
CASP10 antibody	>36 publications with DR5 and CASP10
TP53 antibody	>29 publications with DR5 and TP53
RIPK1 antibody	>21 publications with DR5 and RIPK1
IKBKB antibody	>16 publications with DR5 and IKBKB
IKBKG antibody	>1 publications with DR5 and IKBKG

Pathways associated with anti-DR5 antibody

Products by Pathway	Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Modulation And Signaling Pathway antibodies	Apoptosis Modulation And Signaling Pathway Diagram
Caspase-8 Activation By Cleavage Pathway antibodies	Caspase-8 Activation By Cleavage Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
DNA Damage Response Pathway antibodies	DNA Damage Response Pathway Diagram
Death Receptor Signalling Pathway antibodies	Death Receptor Signalling Pathway Diagram

Diseases associated with anti-DR5 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>2041 publications with DR5 and Necrosis
Neoplasms Antibodies	>1684 publications with DR5 and Neoplasms
Leukemia Antibodies	>248 publications with DR5 and Leukemia
Liver Diseases Antibodies	>163 publications with DR5 and Liver Diseases
Breast Neoplasms Antibodies	>158 publications with DR5 and Breast Neoplasms
Lung Neoplasms Antibodies	>151 publications with DR5 and Lung Neoplasms
Neoplasms, Experimental Antibodies	>143 publications with DR5 and Neoplasms, Experimental
Lung Diseases Antibodies	>141 publications with DR5 and Lung Diseases
Colonic Neoplasms Antibodies	>139 publications with DR5 and Colonic Neoplasms
Inflammation Antibodies	>133 publications with DR5 and Inflammation

Organs/Tissues associated with anti-DR5 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>469 publications with DR5 and Blood
Liver Antibodies	>218 publications with DR5 and Liver
Lung Antibodies	>199 publications with DR5 and Lung
Prostate Antibodies	>162 publications with DR5 and Prostate
Brain Antibodies	>104 publications with DR5 and Brain
Vascular Antibodies	>78 publications with DR5 and Vascular
Kidney Antibodies	>75 publications with DR5 and Kidney
Skin Antibodies	>65 publications with DR5 and Skin
Bone Marrow Antibodies	>57 publications with DR5 and Bone Marrow
Muscle Antibodies	>36 publications with DR5 and Muscle