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CD11a Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488528

Describes the original generation of the antibody and its characterisation through inhibition studies.Recombinant monoclonal antibody to CD11a. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma MHM24. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD11a, Monoclonal Antibody
Full Product Name
Anti-CD11a [MHM24], Mouse IgG1, Kappa
Product Gene Name
anti-CD11a antibody
Product Synonym Names
Integrin alpha-L; CD11 antigen-like family member A; leukocyte function-associated molecule 1 alpha chain; human lymphocyte function antigen
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
MHM24
Host
Mouse
Species Reactivity
Human
Form/Format
PBS with 0.02% Proclin 300
Purity/Purification
Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
MHM24 binds to the CD11a subunit of lymphocyte function-associated antigen. It binds to both the high- and low-affinity forms of the antigen.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
OMIM
153370
3D Structure
ModBase 3D Structure for P20701
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Antibody Type
Recombinant Antibody
Immunogen
Spleen cells from BALB/c mice immunised with EBV-transformed human lymphoblastoid cells were fused with P3-NSUlAg4.1 myeloma cells and the resulting hybridomas were screened for antibody production by a radioimmunoassay on T and B cell lines.
Chimeric Use Statement
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for anti-CD11a antibody

Lympholysis
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CD11a antibody
Cytofluorography, Humanisation, Inhibition studies
Application Notes for anti-CD11a antibody
Cytofluorography was used to show the binding of MHM24 to peripheral blood lymphocytes, with 97.3% of cells stained by MHM24 (Hildreth et al, 1983). In inhibition experiments, MHM24 inhibited T cell-mediated lysis of virus-infected cells (Hildreth et al, 1983). Additionally, MHM24 inhibited NK cell-mediated lysis (Hildreth et al, 1983).Flow cytometry showed MHM24 exhibits non-selective binding to high-affinity and low-affinity forms of LFA-1 (Huang et al, 2006). Murine MHM24 was humanised and shown to prevent adhesion of human T cells to human keratinocytes and the proliferation of T cells in response to nonautologous leukocytes (Werther et al, 1996).

Immunofluorescence (IF) of anti-CD11a antibody

Immunofluorescence staining of Jurkat cells using anti-CD11a antibody MHM24 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of MHM24 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing diffuse membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.
Mouse Anti-CD11a Monoclonal Antibody – Cat# MBS488528

Immunofluorescence (IF) of anti-CD11a antibody

Immunofluorescence staining of Jurkat cells using anti-CD11a MHM24 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells stained with the chimeric mouse IgG version of MHM24 at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-unknown antibody followed by Alexa Fluor 488 secondary antibody.
Mouse Anti-CD11a Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS488528

Product References and Citations for anti-CD11a antibody

Hildreth et al, 1983.; Eur J Immunol. 1983 Mar;13(3):202-8. OriginPubPMID: 8943405

Other Notes

Small volumes of anti-CD11a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD11a purchase

MBS488528 is an antibody targeting CD11a. Also known as Anti-CD11a [MHM24], Mouse IgG1, Kappa. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD11a. It may not necessarily be applicable to this product.

NCBI GI #
88911345
NCBI GeneID
3683
NCBI Accession #
P20701.3
UniProt Primary Accession #
P20701
UniProt Secondary Accession #
O43746; Q45H73; Q96HB1; Q9UBC8
UniProt Related Accession #
P20701
NCBI Official Full Name
Integrin alpha-L
NCBI Official Synonym Full Names
integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
NCBI Official Symbol
ITGAL
NCBI Official Synonym Symbols
CD11A; LFA-1; LFA1A
UniProt Protein Name
Integrin alpha-L
UniProt Synonym Protein Names
CD11 antigen-like family member A; Leukocyte adhesion glycoprotein LFA-1 alpha chain; LFA-1A; Leukocyte function-associated molecule 1 alpha chain; CD_antigen: CD11a
Protein Family
Integrin
UniProt Gene Name
ITGAL
UniProt Synonym Gene Names
CD11A; LFA-1A
UniProt Entry Name
ITAL_HUMAN
NCBI Protein Information
integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD

NCBI Summary for CD11a

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD11a

ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; membrane; plasma membrane; immunological synapse; intercellular junction; integrin complex; external side of plasma membrane

Molecular Function: protein binding; protein heterodimerization activity; metal ion binding; protein complex binding; ICAM-3 receptor activity; cell adhesion molecule binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; regulation of immune response; cell-matrix adhesion; activated T cell proliferation; positive regulation of calcium-mediated signaling; signal transduction; receptor clustering; heterophilic cell adhesion; leukocyte adhesion; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; positive regulation of T cell proliferation; inflammatory response; blood coagulation; cell adhesion; cell motility; leukocyte migration
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Research Articles on CD11a

1. ITGAL is overexpressed in systemic lupus erythematosus CD4+ T cells, but expression is not linked to the typical clinical and serological parameters associated with the disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD11a antibody

 Reference Product  PubMed Publications
 ITGB2 antibody  >1095 publications with CD11a and ITGB2
 ICAM1 antibody  >474 publications with CD11a and ICAM1
 PTPRC antibody  >194 publications with CD11a and PTPRC
 ICAM2 antibody  >41 publications with CD11a and ICAM2
 ICAM3 antibody  >37 publications with CD11a and ICAM3
 ITGAD antibody  >12 publications with CD11a and ITGAD
 ICAM4 antibody  >4 publications with CD11a and ICAM4
 CD226 antibody  >3 publications with CD11a and CD226
 ICAM5 antibody  >2 publications with CD11a and ICAM5

Pathways associated with anti-CD11a antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Focal Adhesion Pathway antibodies  Focal Adhesion Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CD11a antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >244 publications with CD11a and Inflammation
 Necrosis Antibodies  >218 publications with CD11a and Necrosis
 Lung Diseases Antibodies  >69 publications with CD11a and Lung Diseases
 Neoplasms, Experimental Antibodies  >35 publications with CD11a and Neoplasms, Experimental
 Heart Diseases Antibodies  >26 publications with CD11a and Heart Diseases
 Kidney Diseases Antibodies  >25 publications with CD11a and Kidney Diseases
 Atherosclerosis Antibodies  >24 publications with CD11a and Atherosclerosis
 Colitis Antibodies  >21 publications with CD11a and Colitis
 Fibrosis Antibodies  >15 publications with CD11a and Fibrosis
 Leukemia, Promyelocytic, Acute Antibodies  >15 publications with CD11a and Leukemia, Promyelocytic, Acute

Organs/Tissues associated with anti-CD11a antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1562 publications with CD11a and Blood
 Bone Marrow Antibodies  >183 publications with CD11a and Bone Marrow
 Lung Antibodies  >117 publications with CD11a and Lung
 Lymph Antibodies  >86 publications with CD11a and Lymph
 Lymph Node Antibodies  >78 publications with CD11a and Lymph Node
 Spleen Antibodies  >76 publications with CD11a and Spleen
 Intestine Antibodies  >53 publications with CD11a and Intestine
 Brain Antibodies  >50 publications with CD11a and Brain
 Kidney Antibodies  >48 publications with CD11a and Kidney
 Liver Antibodies  >47 publications with CD11a and Liver
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