Uroplakin 3a (UPK3A) ELISA Kit for Rat

Name: Rat UPK3A ELISA Kit; Uroplakin 3a; MyBioSource
Brand: MyBioSource
SKU: MBS049186
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS049186

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated UPK3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat UPK3A antibody to coat Microelisa Stripplate wells… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Uroplakin 3A (UPK3A), ELISA Kit

Full Product Name

Rat Uroplakin 3A ELISA Kit

Product Gene Name

UPK3A elisa kit

Species Reactivity

Rat

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

0.5 ng/ml - 16 ng/ml.

Sensitivity

0.1 ng/ml.

Specificity

No significant cross-reactivity or interference between Rat UPK3A and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

30,670 Da

OMIM

191830

3D Structure

ModBase 3D Structure for O75631

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated UPK3A concentrations in Rat serum, plasma and other body fluids. Using Purified Rat UPK3A antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add UPK3A and UPK3A antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of UPK3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of UPK3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for UPK3A purchase

MBS049186 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Uroplakin 3A (UPK3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing UPK3A. The ELISA analytical biochemical technique of the MBS049186 kit is based on UPK3A antibody-UPK3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect UPK3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, UPK3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for UPK3A. It may not necessarily be applicable to this product.

NCBI GI #

266456217

NCBI GeneID

7380

NCBI Accession #

NP_001161046.1

UniProt Primary Accession #

O75631

UniProt Secondary Accession #

O60261; Q32N05; Q5TII6; B0QY25

UniProt Related Accession #

O75631

GenBank Nucleotide #

NM_001167574.1

NCBI Official Full Name

uroplakin-3a isoform 2

NCBI Official Synonym Full Names

uroplakin 3A

NCBI Official Symbol

UPK3A

NCBI Official Synonym Symbols

UP3A; UPK3; UPIII; UPIIIA

UniProt Protein Name

Uroplakin-3a

UniProt Synonym Protein Names

Uroplakin III

Protein Family

Uroplakin

UniProt Gene Name

UPK3A

UniProt Synonym Gene Names

UPK3; UP3a; UPIII

UniProt Entry Name

UPK3A_HUMAN

NCBI Protein Information

uroplakin-3a; uroplakin 3; uroplakin III

NCBI Summary for UPK3A

This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

UniProt Comments for UPK3A

UPK3A: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Defects in UPK3A are a cause of renal adysplasia (RADYS); also known as renal agenesis or renal aplasia. Renal agenesis refers to the absence of one (unilateral) or both (bilateral) kidneys at birth. Bilateral renal agenesis belongs to a group of perinatally lethal renal diseases, including severe bilateral renal dysplasia, unilateral renal agenesis with contralateral dysplasia and severe obstructive uropathy. Belongs to the uroplakin-3 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: endoplasmic reticulum membrane; apical plasma membrane; integral to membrane

Biological Process: epithelial cell differentiation; urinary bladder development; water transport; urea transport; cell morphogenesis; sodium ion homeostasis; kidney development; potassium ion homeostasis

Disease: Renal Hypodysplasia/aplasia 1

Research Articles on UPK3A

1. studies suggest that bacteria-induced UPIIIa signaling is a critical mediator of bladder responses to insult by uropathogenic E. coli

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with UPK3A elisa kit

Reference Product	PubMed Publications
UPK1B elisa kit	>9 publications with UPK3A and UPK1B
UPK2 elisa kit	>6 publications with UPK3A and UPK2
UPK1A elisa kit	>5 publications with UPK3A and UPK1A
UPK3B elisa kit	>4 publications with UPK3A and UPK3B
KRT20 elisa kit	>2 publications with UPK3A and KRT20

Diseases associated with UPK3A elisa kit

Disease Name	Pubmed Publications
Urinary Bladder Diseases Antibodies	>29 publications with UPK3A and Urinary Bladder Diseases
Neoplasms Antibodies	>20 publications with UPK3A and Neoplasms
Carcinoma Antibodies	>17 publications with UPK3A and Carcinoma
Urinary Bladder Neoplasms Antibodies	>14 publications with UPK3A and Urinary Bladder Neoplasms
Carcinoma, Transitional Cell Antibodies	>8 publications with UPK3A and Carcinoma, Transitional Cell
Cystitis Antibodies	>6 publications with UPK3A and Cystitis
Neoplasm Metastasis Antibodies	>5 publications with UPK3A and Neoplasm Metastasis
Ovarian Neoplasms Antibodies	>4 publications with UPK3A and Ovarian Neoplasms
Adenocarcinoma Antibodies	>4 publications with UPK3A and Adenocarcinoma
Kidney Diseases Antibodies	>3 publications with UPK3A and Kidney Diseases

Organs/Tissues associated with UPK3A elisa kit

Organ/Tissue Name	Pubmed Publications
Prostate Antibodies	>1 publications with UPK3A and Prostate