Osteocalcin (Oc/BGP) (OC/BGP) ELISA Kit for Human

Principle of the Assay: This kit is based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody is pre-coated onto 96-well plates and the biotin conjugated antibody is used as detection antibodies. The standards, test samples and biotin conjugated detectionantibody are added to the wells subsequently, and washed with wash buffer. HRP -Streptavidin is added and unbound conjugatesare washed away with wash buffer. TMB substrates are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP toproduce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

Small volumes of OC/BGP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS495735 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteocalcin (OC/BGP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OC/BGP. The ELISA analytical biochemical technique of the MBS495735 kit is based on OC/BGP antibody-OC/BGP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OC/BGP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OC/BGP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).