Rhodopsin (RHO) Antibody – Mouse Monoclonal

Monoclonal

IgG1

1D4

Mouse

Most mammalian

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.

Protein G purified (Protein G purified culture supernatant.)

Specific for the ~ 39k rhodopsin protein.

Store at -20 degree C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.

39

Chromosome: 22; AC_000179.1 (56867887..56872817, complement). Location: 22q23-q24

ModBase 3D Structure for P02699

Bovine rhodopsin

Purified native bovine rhodopsin

Bovine

The antibody has been directly tested for reactivity in mammals and amphibians.

Rhodopsin is a photoreceptor protein found in retinal rods. It is a complex formed by the binding of retinaldehyde, the oxidized form of retinol, to the protein opsin and undergoes a series of complex reactions in response to visible light resulting in the transmission of nerve impulses to the brain. Mutation of the rhodopsin gene is a major contributor to various retinopathies such as retinitis pigmentosa. The disease-causing protein generally aggregates with ubiquitin in inclusion bodies, disrupts the intermediate filament network and impairs the ability of the cell to degrade non-functioning proteins which leads to photoreceptor apoptosis (Berson et al., 1991). Other mutations on rhodopsin lead to X-linked congenital stationary night blindness, mainly due to constitutive activation, when the mutations occur around the chromophore binding pocket of rhodopsin (Dryja et al.,1993). Several other pathological states relating to rhodopsin have been discovered including poor post-Golgi trafficking, dysregulative activation, rod outer segment instability and arrestin binding.