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Calcitonin Gene Related Peptide (CGRP) (CALCA) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500026

MBS500026 is an antibody targeting CALCA. Also known as Anti-Calcitonin Gene Related Peptide (CGRP). Reactive in Human, rat. Host: Mouse. Mouse monoclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Calcitonin Gene Related Peptide (CGRP) (CALCA), Monoclonal Antibody
Full Product Name
Anti-Calcitonin Gene Related Peptide (CGRP)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4901
Host
Mouse
Species Reactivity
Human, rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western blots.
Purity/Purification
Protein G purified
Specificity
Specific for the CGRP protein in rat DRG neurons.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
Molecular Weight
13,948 Da
Chromosome Location
Chromosome: 1; NC_005100.3 (191158061..191162954, complement). Location: 1q34
3D Structure
ModBase 3D Structure for P01256
Antigen
Rat alpha-CGRP
Immunogen Information
Purified rat alpha-CGRP
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Most mammals
Species Reactivity Note
The antibody has been directly tested for reactivity in rat and human. Other species have not yet been tested. It is expected that the antibody will react with most mammals.
Biological Significance
Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in immunoneutralization (Wong et al., 1993)
Applications Tested/Suitable for anti-CALCA antibody
Immunocytochemistry (ICC)
Application Notes for anti-CALCA antibody
Quality Control: ICC performed on each lot.
WB: not recommended
ICC: 1:50-1:100

Testing Data of anti-CALCA antibody

Mouse Anti-CALCA Monoclonal Antibody – Cat# MBS500026

Product References and Citations for anti-CALCA antibody

• Brain SD, Williams TJ, Tippins JR, Morris HR, MacIntyre I (1985) Calcitonin gene related peptide is a potent vasodilator. Nature 313 (5997): 54-6
• Edvinsson L (2003) New therapeutic target in primary headaches—blocking the CGRP receptor. Expert Opin Ther Targets.7(3):377-83
• Wong HC, Tache' Y, Lloud KCK, Yang H, Sternini C, Holzer P, Walsh JH (1993) Monoclonal antibody to rat alpha-CGRP: production, charactrization, and in vivo immunoneutralization activity. Hybridoma. 12:93-106.

Other Notes

Small volumes of anti-CALCA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CALCA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calcitonin gene-related peptide 1 isoform Cgrp preproprotein
NCBI Official Synonym Full Names
calcitonin-related polypeptide alpha
NCBI Official Symbol
Calca
NCBI Official Synonym Symbols
CAL6; CGRP; Cal1; Calc; RATCAL6; calcitonin
UniProt Protein Name
Calcitonin gene-related peptide 1
UniProt Synonym Protein Names
Alpha-type CGRP; Calcitonin gene-related peptide I
Protein Family
UniProt Gene Name
Calca
UniProt Synonym Gene Names
Calc; CGRP-I
UniProt Entry Name
CALCA_RAT
NCBI Protein Information
calcitonin gene-related peptide 1; CGRP-I; alpha-type CGRP; calcitonin gene-related peptide I; calcitonin/calcitonin-related polypeptide, alpha

NCBI Summary for CALCA

an inhibitor of lactotroph function and a potent vasodilator [RGD, Feb 2006]

UniProt Comments for CALCA

Function: CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.

Subcellular location: Secreted.

Sequence similarities: Belongs to the calcitonin family.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CALCA antibody

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