Mouse Anti-COBRA1 Monoclonal Antibody – Cat# MBS500037

COBRA1 Antibody – Mouse Monoclonal

Name: COBRA1 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS500037
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500037

MBS500037 is an antibody targeting COBRA1. Also known as Anti-COBRA 1. Reactive in Human, mouse, rat. Host: Mouse. Purified mouse monoclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

COBRA1 (NELF-B), Monoclonal Antibody

Full Product Name

Anti-COBRA 1

Product Gene Name

anti-COBRA1 antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

F7E4

Host

Mouse

Species Reactivity

Human, mouse, rat

Form/Format

100 ul in PBS + 0.05 % NaN3. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Protein G purified (Protein G purified culture supernatant)

Specificity

Specific for the ~62k COBRA1 protein in Western blots of neonatal rat brain lysate.

Preparation and Storage

Store at -20 degree C; stable for at least one year

Molecular Weight

Chromosome Location

Chromosome: 9; NC_000009.12 (137255307..137273548). Location: 9q34

OMIM

611180

3D Structure

ModBase 3D Structure for Q8WX92

Antigen

Recombinant full length human COBRA1 purified from E. coli

Immunogen Information

Recombinant full length human COBRA1 purified from E. coli

Immunogen Species

Human

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.

Biological Significance

COBRA1 (cofactor of BRCA1) also known as NELF-B, is an essential component of the negative elongation factor (NELF) complex. COBRA1 has been shown to directly bind estrogen receptor alpha (ERalpha) and repress ERalpha-mediated transcription (Aiyar SE et al., 2004). COBRA1 has also been demonstrated to interact with c-Jun and c-Fos suggesting a role in modulation of the AP-1 pathway and therefore may be important in cell proliferation, differentiation, apoptosis and oncogeneis (Zhong H et al., 2004)

Applications Tested/Suitable for anti-COBRA1 antibody

Western Blot (WB)

Application Notes for anti-COBRA1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-COBRA1 antibody

• Aiyar, SE, Sun J, Blair AL, Moskaluk CA, Lu Y, Ye Q, Yamaguchi Y, Mukherjee A, Ren D, Handa H, Li R (2004). Attenuation of estrogen receptor alpha-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor. Genes Dev. 18 (17): 2134- 46.
• Zhong, H, Zhu J, Zhang H, Ding L, Sun Y, Huang C, Ye Qi (2004). COBRA1 inhibits AP-1 transcriptional activity in transfected cells. Biochem. Biophys. Res. Commun.325 (2): 568- 73.

Other Notes

Small volumes of anti-COBRA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for COBRA1. It may not necessarily be applicable to this product.

NCBI GI #

20070260

NCBI GeneID

25920

NCBI Accession #

NP_056271.2

UniProt Primary Accession #

Q8WX92

UniProt Secondary Accession #

Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3

UniProt Related Accession #

Q8WX92

GenBank Nucleotide #

NM_015456.3

NCBI Official Full Name

negative elongation factor B

NCBI Official Synonym Full Names

negative elongation factor complex member B

NCBI Official Symbol

NELFB

NCBI Official Synonym Symbols

COBRA1; NELF-B; RP13-122B23.3

UniProt Protein Name

Negative elongation factor B

UniProt Synonym Protein Names

Cofactor of BRCA1

UniProt Gene Name

NELFB

UniProt Synonym Gene Names

COBRA1; KIAA1182; NELF-B

UniProt Entry Name

NELFB_HUMAN

NCBI Protein Information

negative elongation factor B; cofactor of BRCA1; negative elongation factor protein B

NCBI Summary for COBRA1

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

UniProt Comments for COBRA1

Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding. Ref.7 Ref.8

Subunit structure: The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-Cor isoform NELF-D)and NELFE. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191. Ref.1 Ref.7 Ref.15

Subcellular location: Nucleus Ref.1.

Tissue specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas. Ref.7

Sequence similarities: Belongs to the NELF-B family.

Sequence caution: The sequence AAH11892.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB14157.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB43381.3 differs from that shown. Reason: Erroneous initiation.

Research Articles on COBRA1

1. These studies allow us to position the actions of two new modulators of GR-regulated transactivation, NELF-A and NELF-B, relative to other factors in the overall gene induction sequence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-COBRA1 antibody

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