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PROX1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500038

MBS500038 is an antibody targeting PROX1. Also known as Anti-Prox1. Reactive in Chicken, human, mouse, rat. Host: Mouse. Mouse monoclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Prox1, Monoclonal Antibody
Full Product Name
Anti-Prox1
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5G10
Host
Mouse
Species Reactivity
Chicken, human, mouse, rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Protein G purified (Protein G purified culture supernatant.)
Specificity
Specifically labels prox1 in formalin-fixed, paraffin-embedded tissue.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
Molecular Weight
83,203 Da
Chromosome Location
Chromosome: 1; NC_000001.11 (213987935..214041510). Location: 1q41
OMIM
601546
3D Structure
ModBase 3D Structure for Q92786
Antigen
Recombinant human Prox1 protein.
Immunogen Information
Recombinant human Prox1 protein
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in human, rat, mouse and chicken. It is expected to have wide species cross reactivity.
Biological Significance
Prox1 (Prospero-related homeobox 1) is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development. The protein is expressed in the developing lens and is also detected in embryonic brain, lung, liver and kidney; while in adult it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas (Chen et al., 2006). Prox1 is thought to play a fundamental role in early development of the CNS and may regulate gene expression and development of postmitotic undifferentiated young neurons (Misra et al., 2008)
Applications Tested/Suitable for anti-PROX1 antibody
Immunohistochemistry (IHC)
Application Notes for anti-PROX1 antibody
Quality Control: IHC performed on each lot.
IHC: 1:1,000

Testing Data of anti-PROX1 antibody

Mouse Anti-PROX1 Monoclonal Antibody – Cat# MBS500038

Product References and Citations for anti-PROX1 antibody

• Xiaoren Chen, Tapan P. Patel, William J. Cain, Melinda K. Duncan. Production of monoclonal antibodies against Prox1. Hybridoma (2006) Feb;25(1):27-33.
• Misra K, Gui H, Matise MP (2008) Prox1 regulates a transitory state for interneuron neurogenesis in the spinal cord. Dev Dyn 237(2):393-402.

Other Notes

Small volumes of anti-PROX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PROX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
prospero homeobox protein 1
NCBI Official Synonym Full Names
prospero homeobox 1
NCBI Official Symbol
PROX1
UniProt Protein Name
Prospero homeobox protein 1
UniProt Synonym Protein Names
Homeobox prospero-like protein PROX1
UniProt Gene Name
PROX1
UniProt Synonym Gene Names
PROX-1
UniProt Entry Name
PROX1_HUMAN
NCBI Protein Information
prospero homeobox protein 1; prospero-related homeobox 1; homeobox prospero-like protein PROX1

NCBI Summary for PROX1

The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]
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UniProt Comments for PROX1

Function: May play a fundamental role in early development of CNS. May regulate gene expression and development of postmitotic undifferentiated young neurons

By similarity.

Subcellular location: Nucleus

Probable.

Tissue specificity: Most actively expressed in the developing lens. Detected also in embryonic brain, lung, liver and kidney. In adult, it is more abundant in heart and liver than in brain, skeletal muscle, kidney and pancreas.

Sequence similarities: Belongs to the Prospero homeobox family.Contains 1 Prospero-type homeobox DNA-binding domain.

Sequence caution: The sequence CAI15309.1 differs from that shown. Reason: Erroneous gene model prediction.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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