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RBFOX1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500043

MBS500043 is an antibody targeting RBFOX1. Also known as Anti-FOX1 (A2BP1). Reactive in Bovine, human, mouse, rat. Host: Mouse. Mouse monoclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FOX1 (RBFOX1), Monoclonal Antibody
Full Product Name
Anti-FOX1 (A2BP1)
Product Gene Name
Clonality
Monoclonal
Isotype
IgG1
Clone Number
1G10
Host
Mouse
Species Reactivity
Bovine, human, mouse, rat
Form/Format
100 ul in PBS + 10 mM sodium azide.
Purity/Purification
Affinity Purified (Affinity purified from tissue culture supernatant.)
Specificity
Specific for the ~46/48k FOX1 protein doublet.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Molecular Weight
46/48
Chromosome Location
Chromosome: 16; NC_000016.10 (5239870..7713338). Location: 16p13.3
OMIM
605104
3D Structure
ModBase 3D Structure for Q9NWB1
Antigen
Recombinant protein taken from the N-terminus of human FOX1 expressed in and purified from E. Coli.
Immunogen Information
Recombinant protein taken from the N-terminus of human FOX1 expressed in and purified from E. Coli
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in bovine, human, mouse and rat.
Biological Significance
FOX1, also known as ataxin-2 binding protein 1 (A2BP1), is a neuron specific RNA-binding protein that has been shown to regulate a wide range of alternative splicing events implicated in neuronal development and maturation including transcription factors and synaptic proteins (Fogel et al., 2012). Defects in FOX1 have also been associated with autism (Martin et al., 2007), attention-deficit hyperactivity disorder (Elia et al., 2010) and schizophrenia (Xu et al., 2008).
Applications Tested/Suitable for anti-RBFOX1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RBFOX1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:5,000
IF: 1:500

Testing Data of anti-RBFOX1 antibody

Mouse Anti-RBFOX1 Monoclonal Antibody – Cat# MBS500043

Product References and Citations for anti-RBFOX1 antibody

• Elia J, Gai X, Xie HM, Perin JC, Geiger E, Glessner JT, D'arcy M, deBerardinis R, Frackelton E, Kim C, Lantieri F, Muganga BM, Wang L, Takeda T, Rappaport EF, Grant SF, Berrettini W, Devoto M, Shaikh TH, Hakonarson H, White PS. (2010) Rare structural variants found in attention-deficit hyperactivity disorder are preferentially associated with neurodevelopmental genes. Mol Psychiatry. Jun;15(6):637-46.
• Fogel BL, Wexler E, Wahnich A, Friedrich T, Vijayendran C, Gao F, Parikshak N, Konopka G, Geschwind DH. (2012) RBFOX1 regulates both splicing and transcriptional networks in human neuronal development. Hum Mol Genet. Oct 1;21(19):4171-86.
• Martin CL, Duvall JA, Ilkin Y, Simon JS, Arreaza MG, Wilkes K, Alvarez-Retuerto A, Whichello A, Powell CM, Rao K, Cook E, Geschwind DH. (2007) Cytogenetic and molecular characterization of A2BP1/FOX1 as a candidate gene for autism. Am J Med Genet B Neuropsychiatr Genet. Oct 5;144B(7):869-76
• Xu B, Roos JL, Levy S, van Rensburg EJ, Gogos JA, Karayiorgou M. (2008) Strong association of de novo copy number mutations with sporadic schizophrenia. Nat Genet. Jul;40(7):880-5.

Other Notes

Small volumes of anti-RBFOX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RBFOX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA binding protein fox-1 homolog 1 isoform 5
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog (C. elegans) 1
NCBI Official Symbol
RBFOX1
NCBI Official Synonym Symbols
2BP1; FOX1; A2BP1; FOX-1; HRNBP1
UniProt Protein Name
RNA binding protein fox-1 homolog 1
UniProt Synonym Protein Names
Ataxin-2-binding protein 1; Fox-1 homolog A; Hexaribonucleotide-binding protein 1
Protein Family
UniProt Gene Name
RBFOX1
UniProt Synonym Gene Names
A2BP; A2BP1; FOX1; HRNBP1
UniProt Entry Name
RFOX1_HUMAN
NCBI Protein Information
RNA binding protein fox-1 homolog 1; fox-1 homolog A; ataxin 2-binding protein 1; hexaribonucleotide-binding protein 1

NCBI Summary for RBFOX1

The Fox-1 family of RNA-binding proteins is evolutionarily conserved, and regulates tissue-specific alternative splicing in metazoa. Fox-1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. Fox-1 and ataxin-2 are both localized in the trans-Golgi network. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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UniProt Comments for RBFOX1

Function: RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. Ref.7

Subunit structure: Binds to the C-terminus of ATXN2. Ref.1

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity.

Tissue specificity: Predominantly expressed in muscle and brain.

Sequence similarities: Contains 1 RRM (RNA recognition motif) domain.

Sequence caution: The sequence AAF78291.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RBFOX1 antibody

 Reference Product  PubMed Publications
 ATXN2 antibody  >1 publications with RBFOX1 and ATXN2
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