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RBFOX3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500051

MBS500051 is an antibody targeting RBFOX3. Also known as Anti-FOX3 (NeuN). Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
FOX3 (RBFOX3), Monoclonal Antibody
Full Product Name
Anti-FOX3 (NeuN)
Product Gene Name
Product Synonym Names
FOX3 (NeuN)
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1B7
Host
Mouse
Form/Format
100 ul in PBS + 10 mM NaN3.
Purity/Purification
Affinity purified from tissue culture supernatant.
Specificity
Specific for the ~46/48k FOX3 protein doublet.
Preparation and Storage
Store at -20 degree C.
Molecular Weight
~46/48 kDa
3D Structure
ModBase 3D Structure for A6NFN3
Immunogen Species
Human
Immunogen Info
Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E Coli
Biological Significance
FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing (Kim et al., 2009). FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development (Kim et al., 2013).
Antigen
Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E Coli.
Applications Tested/Suitable for anti-RBFOX3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RBFOX3 antibody
WB: 1:2,000
IF: 1:500

Western Blot (WB) of anti-RBFOX3 antibody

Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~46/48k FOX3 protein. Right: Immunofluorescence of cultured rat neurons showing strong nuclear and distal cytoplasmic staining of FOX3 in red and the complete absence of astrocyte staining, which are stained green using our GFAP antibody
Mouse Anti-RBFOX3 Monoclonal Antibody – Cat# MBS500051

Other Notes

Small volumes of anti-RBFOX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RBFOX3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA binding protein fox-1 homolog 3
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog 3
NCBI Official Symbol
RBFOX3
NCBI Official Synonym Symbols
FOX3; NEUN; FOX-3; HRNBP3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
UniProt Synonym Protein Names
Fox-1 homolog C
Protein Family
UniProt Gene Name
RBFOX3
UniProt Entry Name
RFOX3_HUMAN
NCBI Protein Information
RNA binding protein fox-1 homolog 3

UniProt Comments for RBFOX3

NeuN: RNA-binding protein that regulates alternative splicing events.

Protein type: DNA-binding; RNA processing; RNA splicing

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; nucleus; perikaryon

Molecular Function: DNA binding; mRNA binding; nucleotide binding

Biological Process: mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA splicing
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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