Rabbit Anti-NCC Polyclonal Antibody – Cat# MBS500073

Western blot of mouse kidney lysate showing specific immunolabeling of the ~160 kDa NCC protein phosphorylated at Thr53 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (lambda- Ptase, 1200 units for 30 min).

NCC Antibody – Rabbit Polyclonal

Name: NCC Antibody; Polyclonal; Rabbit; Pig-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS500073
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500073

The thiazide-sensitive sodium chloride cotransporter, NCC, is the major NaCl transport protein in the distal convoluted tubule (DCT) and plays an important role in maintaining blood pressure (Rosenbaek et al., 2014, Feng et al., 2015). Phosphorylation of NCC… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NCC, Polyclonal Antibody

Full Product Name

Anti-Phospho-Thr53 NCC Antibody

Product Gene Name

anti-NCC antibody

Product Synonym Gene Name

SLC12A3

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Guinea Pig, Hamster

Form/Format

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

Purity/Purification

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Specificity

Specific for endogenous levels of the ~160 kDa NCC protein phosphorylated at Thr53. Band of interest smearing likely due to glycosylation. Immunolabeling is completely eliminated by treatment with l-Ptase.

Preparation and Storage

Storage at -20 degree C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.
Stable for at least 1 year at -20 degree C.

Molecular Weight

~160 kDa

3D Structure

ModBase 3D Structure for P59158

Immunogen Species

Mouse
Immunogen Information||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr53 of mouse NCC.

Applications Tested/Suitable for anti-NCC antibody

Western Blot (WB)

Application Notes for anti-NCC antibody

WB: 1:1000

Product References and Citations for anti-NCC antibody

Feng X, Zhang Y, Shao N, Wang Y, Zhuang Z, Wu P, Lee MJ, Liu Y, Wang X, Zhuang J, Delpire E, Gu D, Cai H. (2015) Aldosterone modulates thiazide-sensitive sodium chloride cotransporter abundance via DUSP6-mediated ERK1/2 signaling pathway. Am J Physiol Renal Physiol. 308(10):F1119-27
Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA. (2014) Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis. J Biol Chem. 289(19):13347-61

Other Notes

Small volumes of anti-NCC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NCC purchase

MBS500073 is an antibody targeting NCC. Also known as Anti-Phospho-Thr53 NCC Antibody. Reactive in Guinea Pig, Hamster. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for NCC. It may not necessarily be applicable to this product.

NCBI GI #

328887916

NCBI GeneID

20497

NCBI Accession #

NP_001192240.1

UniProt Primary Accession #

P59158

UniProt Related Accession #

P59158

GenBank Nucleotide #

NM_001205311.1

NCBI Official Full Name

solute carrier family 12 member 3 isoform 1

NCBI Official Synonym Full Names

solute carrier family 12, member 3

NCBI Official Symbol

Slc12a3

NCBI Official Synonym Symbols

NCC; TSC; NCCT; AI035291

UniProt Protein Name

Solute carrier family 12 member 3

UniProt Synonym Protein Names

Na-Cl symporter; Thiazide-sensitive sodium-chloride cotransporter

Protein Family

C-C motif chemokine

UniProt Gene Name

Slc12a3

UniProt Synonym Gene Names

Ncc; Tsc

NCBI Protein Information

solute carrier family 12 member 3

UniProt Comments for NCC

Electroneutral sodium and chloride ion cotransporter. In kidney distal convoluted tubules, key mediator of sodium and chloride reabsorption (). Receptor for the proinflammatory cytokine IL18. Contributes to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1 (PubMed:26099046).

Research Articles on NCC

1. Calcineurin is activated by an acute potassium load, which rapidly dephosphorylates NCC, leading to increased urinary potassium excretion.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NCC antibody

Reference Product	PubMed Publications
Wnk4 antibody	>35 publications with NCC and Wnk4
Stk39 antibody	>29 publications with NCC and Stk39
Wnk1 antibody	>27 publications with NCC and Wnk1
Kcnj1 antibody	>24 publications with NCC and Kcnj1
Acacb antibody	>1 publications with NCC and Acacb

Pathways associated with anti-NCC antibody

Products by Pathway	Pathway Diagram
Cation-coupled Chloride Cotransporters Pathway antibodies	Cation-coupled Chloride Cotransporters Pathway Diagram
SLC-mediated Transmembrane Transport Pathway antibodies	SLC-mediated Transmembrane Transport Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram
Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway antibodies	Transport Of Inorganic Cations/anions And Amino Acids/oligopeptides Pathway Diagram

Diseases associated with anti-NCC antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>203 publications with NCC and Kidney Diseases
Gitelman Syndrome Antibodies	>159 publications with NCC and Gitelman Syndrome
Hypertension Antibodies	>107 publications with NCC and Hypertension
Vascular Diseases Antibodies	>71 publications with NCC and Vascular Diseases
Disease Models, Animal Antibodies	>25 publications with NCC and Disease Models, Animal
Nervous System Diseases Antibodies	>23 publications with NCC and Nervous System Diseases
Renal Insufficiency Antibodies	>14 publications with NCC and Renal Insufficiency
Hypertrophy Antibodies	>10 publications with NCC and Hypertrophy
Proteinuria Antibodies	>7 publications with NCC and Proteinuria
Brain Diseases Antibodies	>6 publications with NCC and Brain Diseases

Organs/Tissues associated with anti-NCC antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>246 publications with NCC and Kidney
Mixed Antibodies	>3 publications with NCC and Mixed
Spleen Antibodies	>2 publications with NCC and Spleen
Inner Ear Antibodies	>2 publications with NCC and Inner Ear