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RBPMS Antibody – Guinea Pig Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500074

RBPMS (RNA binding protein with multiple splicing), also known as HERMES, contains one RRM (RNA recognition motif) domain and belongs to the RRM family of RNA-binding proteins. RBPMS exists as multiple alternatively spliced isoforms and is thought to bind… More details

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Product Overview

Product Name
RBPMS, Polyclonal Antibody
Full Product Name
Anti-RBPMS Antibody
Product Gene Name
Product Synonym Gene Name
RBPMS
Clonality
Polyclonal
Host
Guinea Pig
Species Reactivity
Canine, Human, Hamster
Form/Format
PBS + 0.03% NaN3
Purity/Purification
The antibody is prepared from guinea pig serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
Specificity
Specific for endogenous levels of the ~24 kDa RBPMS protein.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year.
Avoid freeze/thaw cycles.
Molecular Weight
~24 kDa
3D Structure
ModBase 3D Structure for Q9WVB0
Immunogen Species
Rat
Immunogen Information||Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Antigen
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH.
Applications Tested/Suitable for anti-RBPMS antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-RBPMS antibody
WB: 1:1000
IHC: 1:500

Western Blot (WB) of anti-RBPMS antibody

Western blot of rat heart lysate showing specific labeling of the ~24 kDa RBPMS protein.
Guinea Pig Anti-RBPMS Polyclonal Antibody – Cat# MBS500074

Immunofluorescence (IF) of anti-RBPMS antibody

Immunostaining of rat retinal ganglion cells showing specific immunolabeling of RBPMS in green. Photo courtesy of Brian Choi, Univ. of Toronto
Guinea Pig Anti-RBPMS Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS500074

Product References and Citations for anti-RBPMS antibody

Rodriguez AR, de Sevilla Müller LP, Brecha NC (2014) The RNA binding protein RBPMS is a selective marker of ganglion cells in the mammalian retina. J Comp Neurol. Apr 15;522(6):1411-43.
Kwong JM, Caprioli J, Piri N (2010) RNA binding protein with multiple splicing: a new marker for retinal ganglion cells. Invest Ophthalmol Vis Sci. Feb;51(2):1052-8.

Other Notes

Small volumes of anti-RBPMS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for RBPMS purchase

MBS500074 is an antibody targeting RBPMS. Reactive in Canine, Human, Hamster. Host: Guinea Pig.

Additional Details

NCBI/Uniprot data below describe general gene information for RBPMS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
RNA-binding protein with multiple splicing isoform 1
NCBI Official Synonym Full Names
RNA binding protein gene with multiple splicing
NCBI Official Symbol
Rbpms
NCBI Official Synonym Symbols
RBP-MS; hermes; AU017537; 2010300K22Rik; 2700019M19Rik
UniProt Protein Name
RNA-binding protein with multiple splicing
UniProt Synonym Protein Names
Heart and RRM expressed sequence; Hermes
UniProt Gene Name
Rbpms
UniProt Synonym Gene Names
Hermes; RBP-MS; Hermes
NCBI Protein Information
RNA-binding protein with multiple splicing

UniProt Comments for RBPMS

Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.
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Precautions

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Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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