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MARCKS Antibody – Chicken Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500078

Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al.,1990) and is concentrated in synapses of neurons. It appears to function in synaptic vesicle cycling and has been shown to… More details

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Product Overview

Online Datasheet in PDF
Product Name
MARCKS, Polyclonal Antibody
Full Product Name
Anti-MARCKS Antibody
Product Gene Name
anti-MARCKS antibody
Clonality
Polyclonal
Host
Chicken
Species Reactivity
Human
Predicted: Non-human primate
Form/Format
Total IgY fraction in PBS + 10 mM NaN3.
Purity/Purification
Total IgY Fraction
Specificity
Specific for endogenous levels of the ~80 kDa MARCKS protein.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Molecular Weight
~80 kDa
OMIM
177061
3D Structure
ModBase 3D Structure for P29966
Immunogen Species
Human
Immunogen Information
Recombinant human MARCKS expressed in and purified from E Coli.
Antigen
Recombinant human MARCKS expressed in and purified from E Coli.
Quality Control Tests
Western blots performed on each lot.
Applications Tested/Suitable for anti-MARCKS antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-MARCKS antibody
WB: 1:10,000
IF: 1:1000

Western Blot (WB) of anti-MARCKS antibody

Western blot of HeLa lysate showing specific immunolabeling of the ~80 kDa MARCKS protein.
Chicken Anti-MARCKS Polyclonal Antibody – Cat# MBS500078

Immunostaining of anti-MARCKS antibody

Immunostaining of HeLa cells showing specific labeling of the MARCKS protein in red and B-tubulin in green.
Chicken Anti-MARCKS Polyclonal Antibody, Immunostaining – Cat# MBS500078

Product References and Citations for anti-MARCKS antibody

1. Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698. 2. Hartwig JH, Thelen M, Rosen A, Janmey PA, Nairn AC and Aderem A. (1992) MARCKS is an actinfilament crosslinking protein regulated by protein kinase C and calcium-calmodulin. Nature 356: 618-622. 3. Stumpo DJ, Bock CB, Tuttle JS and Blackshear PJ. (1995) MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc. Nat. Acad. Sci. 92; 944-948. 4. Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 269:9361-9367.

Other Notes

Small volumes of anti-MARCKS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MARCKS purchase

MBS500078 is an antibody targeting MARCKS. Reactive in Human
Predicted: Non-human primate. Host: Chicken.

Additional Details

NCBI/Uniprot data below describe general gene information for MARCKS. It may not necessarily be applicable to this product.

NCBI GI #
153070260
NCBI GeneID
4082
NCBI Accession #
NP_002347.5
UniProt Primary Accession #
P29966
UniProt Related Accession #
P29966
GenBank Nucleotide #
NM_002356.7
NCBI Official Full Name
myristoylated alanine-rich C-kinase substrate
NCBI Official Synonym Full Names
myristoylated alanine rich protein kinase C substrate
NCBI Official Symbol
MARCKS
NCBI Official Synonym Symbols
MACS; 80K-L; PKCSL; PRKCSL
UniProt Protein Name
Myristoylated alanine-rich C-kinase substrate
UniProt Synonym Protein Names
Protein kinase C substrate, 80 kDa protein, light chain
Protein Family
Myristoylated alanine-rich C-kinase substrate
UniProt Gene Name
MARCKS
UniProt Synonym Gene Names
MACS; PRKCSL; MARCKS; 80K-L protein; PKCSL
UniProt Entry Name
MARCS_HUMAN
NCBI Protein Information
myristoylated alanine-rich C-kinase substrate

NCBI Summary for MARCKS

The protein encoded by this gene is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. Phosphorylation by protein kinase C or binding to calcium-calmodulin inhibits its association with actin and with the plasma membrane, leading to its presence in the cytoplasm. The protein is thought to be involved in cell motility, phagocytosis, membrane trafficking and mitogenesis. [provided by RefSeq, Jul 2008]
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UniProt Comments for MARCKS

MARCKS: the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development. Phosphorylation by PKC displaces it from the plasma membrane, inhibiting F-actin cross-linking activity. Plays a role in growth cone adhesion: its nonphosphorylated form stabilizes integrin-mediated adhesions, and its phosphorylation-triggered release from adhesions causes localized growth cone detachment. Highly expressed in immature T and B lymphocytes: its expression is highest in CD4-8- T cells and B-cell blasts generated by antigen receptor signaling. Its expression and phosphorylation are closely linked to microglial activation processes involved in pathological conditions such as neuroinflammation and neurodegeneration.

Protein type: Motility/polarity/chemotaxis; Actin-binding

Chromosomal Location of Human Ortholog: 6q22.2

Cellular Component: germinal vesicle; centrosome; focal adhesion; plasma membrane; cell cortex; actin cytoskeleton

Molecular Function: calmodulin binding; actin filament binding; protein kinase C binding

Biological Process: energy reserve metabolic process; regulation of insulin secretion
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Research Articles on MARCKS

1. High MARCKS expression is associated with liver tumor.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MARCKS antibody

 Reference Product  PubMed Publications
 PRKCA antibody  >32 publications with MARCKS and PRKCA
 TOB1 antibody  >1 publications with MARCKS and TOB1

Pathways associated with anti-MARCKS antibody

 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Fc Gamma R-mediated Phagocytosis Pathway antibodies  Fc Gamma R-mediated Phagocytosis Pathway Diagram
 Integration Of Energy Metabolism Pathway antibodies  Integration Of Energy Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Regulation Of Insulin Secretion Pathway antibodies  Regulation Of Insulin Secretion Pathway Diagram
 Regulation Of Insulin Secretion By Acetylcholine Pathway antibodies  Regulation Of Insulin Secretion By Acetylcholine Pathway Diagram

Diseases associated with anti-MARCKS antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >85 publications with MARCKS and Neoplasms
 Nervous System Diseases Antibodies  >37 publications with MARCKS and Nervous System Diseases
 Brain Diseases Antibodies  >23 publications with MARCKS and Brain Diseases
 Inflammation Antibodies  >20 publications with MARCKS and Inflammation
 Disease Models, Animal Antibodies  >15 publications with MARCKS and Disease Models, Animal
 Necrosis Antibodies  >12 publications with MARCKS and Necrosis
 Adenocarcinoma Antibodies  >9 publications with MARCKS and Adenocarcinoma
 Drug Toxicity Antibodies  >9 publications with MARCKS and Drug Toxicity
 Neoplasms, Experimental Antibodies  >9 publications with MARCKS and Neoplasms, Experimental
 Neural Tube Defects Antibodies  >8 publications with MARCKS and Neural Tube Defects

Organs/Tissues associated with anti-MARCKS antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >220 publications with MARCKS and Brain
 Vascular Antibodies  >35 publications with MARCKS and Vascular
 Lung Antibodies  >27 publications with MARCKS and Lung
 Embryonic Tissue Antibodies  >20 publications with MARCKS and Embryonic Tissue
 Kidney Antibodies  >17 publications with MARCKS and Kidney
 Heart Antibodies  >16 publications with MARCKS and Heart
 Liver Antibodies  >16 publications with MARCKS and Liver
 Bone Antibodies  >16 publications with MARCKS and Bone
 Eye Antibodies  >15 publications with MARCKS and Eye
 Intestine Antibodies  >12 publications with MARCKS and Intestine
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