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CRISPR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500083

CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes.… More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CRISPR Cas9, Monoclonal Antibody
Full Product Name
Anti-CRISPR Cas9 Antibody
Product Gene Name
anti-CRISPR antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6F7
Host
Mouse
Form/Format
100 ul in PBS + 5 mM NaN3
Purity/Purification
Protein G Purified Culture Supernatant
Specificity
Specific for the Staphylococcus aureus Cas9 protein
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Immunogen Species
Mouse
Immunogen Information
Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
Antigen
Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
Quality Control Tests
Western blots performed on each lot.
Applications Tested/Suitable for anti-CRISPR antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-CRISPR antibody
WB: 1:1000
IF: 1:500

Western Blot (WB) of anti-CRISPR antibody

Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).
Mouse Anti-CRISPR Monoclonal Antibody – Cat# MBS500083

Immunofluorescence (IF) of anti-CRISPR antibody

Immunofluorescence of HEK293 cells transfected with Cas9 and labeled with Cas9 antibody.
Mouse Anti-CRISPR Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS500083

Product References and Citations for anti-CRISPR antibody

Ran FA, Cong L, Yan WX, Scott DA, Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F. (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186-91.

Other Notes

Small volumes of anti-CRISPR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CRISPR purchase

MBS500083 is an antibody targeting CRISPR. Also known as Anti-CRISPR Cas9 Antibody. Host: Mouse.
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