Rabbit Anti-AQP2 Polyclonal Antibody – Cat# MBS502006

Aquaporin 2 (Ser261) (AQP2) Antibody – Rabbit Polyclonal

Name: AQP2 Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502006
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502006

MBS502006 is an antibody targeting AQP2. Also known as Anti-Phospho-Ser261 Aquaporin 2. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Aquaporin 2 (Ser261) (AQP2), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser261 Aquaporin 2

Product Gene Name

anti-AQP2 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for ~29k AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.

Preparation and Storage

Store at -20 degree C; stable for at least one year.

Molecular Weight

29/37

Chromosome Location

Chromosome: X; NC_005120.3 (115742312..115747347). Location: chromosome: X

3D Structure

ModBase 3D Structure for P34080

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Canine and bovine, chicken, human, mouse, non-human primate

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, bovine, chicken, human, mouse and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007).

Applications Tested/Suitable for anti-AQP2 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-AQP2 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:100 (Hoffert et al., 2007)

Product References and Citations for anti-AQP2 antibody

• van Balkom BW, Savelkoul PJ, Markovich D, Hofman E, Nielsen S, van der Sluijs P, Deen PM (2002) The role of putative phosphorylation sites in the targeting and shuttling of the aquaporin 2 water channel. J Biol Chem 277(44):41473-9.
• Ford P, Rivarola V, Chara O, Blot-Chabaud M, Cluzeaud F, Farman M, Parisi M, Capurro C (2005) Volume regulation in cortical collecting duct cells: role of AQP2. Biol Cell 97(9):687-97.
• Hoffert JD, Nielsen J,, Yu MJ,, Pisitikun T,, Schleicher SM,, Nielsen Knepper MA (2007) Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct.. Am J Physiol Renal Physiol 292: F691-F700..
• Iolascon A, Aglio V, Tamma G, D'Appolito M, Addabbo F, Procino G, Simonetti MC, Montini G, Gesulado L, Debler EW, Suelto M, Valenti G (2007) Characterization of two novel missense mutations in AQP2 gene causing nephrogenic diabetes insipidus. Nephron Physiol. 105(3): p33-41.
• Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA. (2008) Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27

Other Notes

Small volumes of anti-AQP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AQP2. It may not necessarily be applicable to this product.

NCBI GI #

77539434

NCBI GeneID

25386

NCBI Accession #

NP_037041.2

UniProt Primary Accession #

P34080

UniProt Secondary Accession #

A1A5L4

UniProt Related Accession #

P34080

GenBank Nucleotide #

NM_012909.2

NCBI Official Full Name

aquaporin-2

NCBI Official Synonym Full Names

aquaporin 2 (collecting duct)

NCBI Official Symbol

Aqp2

NCBI Official Synonym Symbols

AQP-2; aquaporin-2

UniProt Protein Name

Aquaporin-2

UniProt Synonym Protein Names

ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct

UniProt Gene Name

Aqp2

UniProt Synonym Gene Names

AQP-2; AQP-CD

UniProt Entry Name

AQP2_RAT

NCBI Protein Information

aquaporin-2; AQP-CD; WCH-CD; aquaporin-CD; ADH water channel; collecting duct water channel protein; water channel protein for renal collecting duct

NCBI Summary for AQP2

water channel protein involved in water reabsorption and vasopressin regulation in kidney collecting duct cells; mutation is linked to nephrogenic diabetes insipidous (NDI) [RGD, Feb 2006]

UniProt Comments for AQP2

Function: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Subcellular location: Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Golgi apparatus › trans-Golgi network membrane; Multi-pass membrane protein. Note: Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated. Ref.6

Tissue specificity: Expressed in renal collecting tubules.

Domain: Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Post-translational modification: Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent

By similarity.

Sequence similarities: Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Sequence caution: The sequence AAA41478.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on AQP2

1. These data suggest that an increased plasma level of vasopressin promoted the excretion of urinary exosomal AQP2 and that urine alkalinization also increased it independently of vasopressin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AQP2 antibody

Reference Product	PubMed Publications
Aqp1 antibody	>240 publications with AQP2 and Aqp1
Aqp3 antibody	>216 publications with AQP2 and Aqp3
Aqp4 antibody	>137 publications with AQP2 and Aqp4
Trpc6 antibody	>2 publications with AQP2 and Trpc6
Trpc3 antibody	>2 publications with AQP2 and Trpc3
Myo5b antibody	>1 publications with AQP2 and Myo5b

Pathways associated with anti-AQP2 antibody

Products by Pathway	Pathway Diagram
Aquaporin-mediated Transport Pathway antibodies	Aquaporin-mediated Transport Pathway Diagram
Passive Transport By Aquaporins Pathway antibodies	Passive Transport By Aquaporins Pathway Diagram
Regulation Of Water Balance By Renal Aquaporins Pathway antibodies	Regulation Of Water Balance By Renal Aquaporins Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram
Vasopressin-regulated Water Reabsorption Pathway antibodies	Vasopressin-regulated Water Reabsorption Pathway Diagram
Vasopressin-regulated Water Reabsorption Pathway antibodies	Vasopressin-regulated Water Reabsorption Pathway Diagram

Diseases associated with anti-AQP2 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>324 publications with AQP2 and Kidney Diseases
Diabetes Insipidus, Nephrogenic Antibodies	>240 publications with AQP2 and Diabetes Insipidus, Nephrogenic
Polyuria Antibodies	>128 publications with AQP2 and Polyuria
Disease Models, Animal Antibodies	>88 publications with AQP2 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>69 publications with AQP2 and Cardiovascular Diseases
Heart Failure Antibodies	>52 publications with AQP2 and Heart Failure
Hypertension Antibodies	>47 publications with AQP2 and Hypertension
Heart Diseases Antibodies	>45 publications with AQP2 and Heart Diseases
Brain Diseases Antibodies	>36 publications with AQP2 and Brain Diseases
Renal Insufficiency Antibodies	>30 publications with AQP2 and Renal Insufficiency

Organs/Tissues associated with anti-AQP2 antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>862 publications with AQP2 and Kidney
Prostate Antibodies	>4 publications with AQP2 and Prostate