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Dynamin (DNM1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502027

MBS502027 is an antibody targeting DNM1. Also known as Anti-Dynamin. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Dynamin (DNM1), Polyclonal Antibody
Full Product Name
Anti-Dynamin
Product Gene Name
anti-DNM1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.)
Specificity
Specific for ~95k dynamin protein.
Preparation and Storage
Store at -20 degree C; stable for at least one year.
Molecular Weight
95
Chromosome Location
Chromosome: 9; NC_000009.12 (128203355..128255249). Location: 9q34
OMIM
602377
3D Structure
ModBase 3D Structure for Q05193
Antigen
Peptide corresponding to amino acid residues from the N-terminal region of human Dynamin, conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH
Immunogen Species
Human
Reactivity Assumed Based on 100% Sequence Homology
Bovine, chicken, human, mouse and non-human primate
Species Reactivity Note
The antibody has been demonstrated to work in rat. It is also anticipated that the antibody will work with human, mouse, non-human primates, cow and chicken based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). There are 3 known isofoms of Dynamin, each having several splice variants as well. Dynamin I is expressed only in neurons whereas Dynamin II is ubiquitously expressed and Dynamin III is found primarily in the testes. Dynamin 1 is phosphorylated by PKC and dephosphorylated by calcineurin.
Applications Tested/Suitable for anti-DNM1 antibody
Western Blot (WB)
Application Notes for anti-DNM1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-DNM1 antibody

Rabbit Anti-DNM1 Polyclonal Antibody – Cat# MBS502027

Product References and Citations for anti-DNM1 antibody

• Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.
• Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.
• Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.
• Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem 277:15957-15961.

Other Notes

Small volumes of anti-DNM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DNM1. It may not necessarily be applicable to this product.

NCBI GI #
56549117
NCBI GeneID
1759
NCBI Accession #
NP_001005336.1
UniProt Primary Accession #
Q05193
UniProt Secondary Accession #
Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2; A6NLM6
UniProt Related Accession #
Q05193
GenBank Nucleotide #
NM_001005336.2
NCBI Official Full Name
dynamin-1 isoform 2
NCBI Official Synonym Full Names
dynamin 1
NCBI Official Symbol
DNM1
NCBI Official Synonym Symbols
DNM
UniProt Protein Name
Dynamin-1
Protein Family
Dynamin
UniProt Gene Name
DNM1
UniProt Synonym Gene Names
DNM
UniProt Entry Name
DYN1_HUMAN
NCBI Protein Information
dynamin-1

NCBI Summary for DNM1

This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for DNM1

Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.

Catalytic activity: GTP + H2O = GDP + phosphate.

Subunit structure: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3

By similarity. Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Interacts with SNX9. Interacts with MYO1E (via SH3 domain). Interacts with SNX33 (via SH3 domain). Ref.4 Ref.6 Ref.7

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Ref.4

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.Contains 1 PH domain.
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Research Articles on DNM1

1. The discovery that the pre-mRNA sequence of dnm1 in humans has sequence features similar to that of the alternative splicing patterns observed in insects greatly expands the applicability of the docking site-selector sequence pairing model to bilaterian animals.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DNM1 antibody

 Reference Product  PubMed Publications
 GRB2 antibody  >1 publications with DNM1 and GRB2
 SH3GL2 antibody  >1 publications with DNM1 and SH3GL2

Pathways associated with anti-DNM1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 CXCR3-mediated Signaling Events Pathway antibodies  CXCR3-mediated Signaling Events Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 EPHB Forward Signaling Pathway antibodies  EPHB Forward Signaling Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram

Diseases associated with anti-DNM1 antibody

 Disease Name  Pubmed Publications
 Dog Diseases Antibodies  >4 publications with DNM1 and Dog Diseases
 Disease Models, Animal Antibodies  >3 publications with DNM1 and Disease Models, Animal
 Brain Diseases Antibodies  >3 publications with DNM1 and Brain Diseases
 Nervous System Malformations Antibodies  >2 publications with DNM1 and Nervous System Malformations
 Kidney Diseases Antibodies  >2 publications with DNM1 and Kidney Diseases
 Cognition Disorders Antibodies  >1 publications with DNM1 and Cognition Disorders
 Neurobehavioral Manifestations Antibodies  >1 publications with DNM1 and Neurobehavioral Manifestations
 Memory Disorders Antibodies  >1 publications with DNM1 and Memory Disorders
 Necrosis Antibodies  >1 publications with DNM1 and Necrosis
 Fatty Liver Antibodies  >1 publications with DNM1 and Fatty Liver

Organs/Tissues associated with anti-DNM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >8 publications with DNM1 and Nerve
 Muscle Antibodies  >6 publications with DNM1 and Muscle
 Brain Antibodies  >5 publications with DNM1 and Brain
 Kidney Antibodies  >3 publications with DNM1 and Kidney
 Embryonic Tissue Antibodies  >3 publications with DNM1 and Embryonic Tissue
 Testis Antibodies  >2 publications with DNM1 and Testis
 Eye Antibodies  >1 publications with DNM1 and Eye
 Pancreas Antibodies  >1 publications with DNM1 and Pancreas
 Connective Tissue Antibodies  >1 publications with DNM1 and Connective Tissue
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