Sheep Anti-DNM1 Polyclonal Antibody – Cat# MBS502028

Dynamin (Ser774) (DNM1) Antibody – Sheep Polyclonal

Name: DNM1 Antibody; Polyclonal; Sheep; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502028
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502028

MBS502028 is an antibody targeting DNM1. Also known as Anti-Phospho-Ser774 Dynamin. Reactive in Rat. Host: Sheep. Affinity purified sheep polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Dynamin (Ser774) (DNM1), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser774 Dynamin

Product Gene Name

anti-DNM1 antibody

Clonality

Polyclonal

Host

Sheep

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

Chromosome Location

Chromosome: 3; NC_005102.3 (16686951..16730913, complement). Location: 3p11

3D Structure

ModBase 3D Structure for P21575

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat dynamin.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser774 conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Bovine, canine, chicken, human, mouse and non-human primates

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of Ser774 by GSK3 has recently been shown to control activity-dependent bulk endocytosis of synaptic vesicles (Clayton et al., 2010)

Applications Tested/Suitable for anti-DNM1 antibody

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Application Notes for anti-DNM1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (fixed cultured neurons, Tan et al., 2003): 1:1000
IF (fixed cultured neurons; Tan et al., 2003): 1:1000

Product References and Citations for anti-DNM1 antibody

• Cousin MA, Tan TC, Robinson PJ (2001) Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. J Neurochem 76:105-116.
• Graham ME, O'Callaghan DW, McMahon HT, Burgoyne RD (2002) Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proc Natl Acad Sci USA 99:7124-7129.
• Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) cdk5 is essential for synaptic vesicle endocytosis. Nat Cell Biol 5:701-710.
• Tsuboi T, Terakawa S, Scalettar BA, Fantus C, Roder J, Jeromin A (2002) Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. J Biol Chem 277:15957-15961.
• Clayton EL, Sue N, Smillie KJ, O'Leary T, Bache N, Cheung G, Cole AR, Wyllie DJ, Sutherland C, Robinson PJ, Cousin MA. (2010) Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles. Nat Neurosci. 13(7):845-51.

Other Notes

Small volumes of anti-DNM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DNM1. It may not necessarily be applicable to this product.

NCBI GI #

18093102

NCBI GeneID

140694

NCBI Accession #

NP_542420.1

UniProt Primary Accession #

P21575

UniProt Related Accession #

P21575

GenBank Nucleotide #

NM_080689.4

NCBI Official Full Name

dynamin-1

NCBI Official Synonym Full Names

dynamin 1

NCBI Official Symbol

Dnm1

NCBI Official Synonym Symbols

Dnm

UniProt Protein Name

Dynamin-1

UniProt Synonym Protein Names

B-dynamin; D100; Dynamin, brain

Protein Family

Dynamin

UniProt Gene Name

Dnm1

UniProt Synonym Gene Names

Dnm

UniProt Entry Name

DYN1_RAT

NCBI Protein Information

dynamin-1; D100; B-dynamin; dynamin, brain

NCBI Summary for DNM1

mediates GTP hydrolysis; plays a role in receptor mediated endocytosis; required for agonist-induced internalization of the epidermal growth factor receptor [RGD, Feb 2006]

UniProt Comments for DNM1

Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis

By similarity.

Catalytic activity: GTP + H2O = GDP + phosphate.

Subunit structure: Interacts with CAV1 and SH3GLB1. Interacts with SNX9. Interacts with SNX33 (via SH3 domain)

By similarity. Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Binds SH3GL1, SH3GL2 and SH3GL3. Interacts with MYO1E (via SH3 domain). Ref.6 Ref.7 Ref.10

Subcellular location: Cytoplasm Ref.8. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Isoform-specific localization. Ref.8Isoform 2: Cytoplasm Ref.8. Isoform 6: Cytoplasm Ref.8. Golgi apparatus Ref.8.

Tissue specificity: Brain-specific (peripheral sensory neurons). Ref.8

Developmental stage: Expressed in neurons after maturation.

Post-translational modification: Constitutively phosphorylated by CDK5 in nerve terminals and dephosphorylated by calcineurin when synaptic vesicle endocytosis (SVE) is stimulated by depolarization-dependent calcium influx. Ref.3 Ref.9 Ref.11

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.Contains 1 PH domain.

Caution: Absence of tyrosine and threonine phosphorylation is demonstrated in Ref.3. However, tyrosine and threonine phosphorylation may still occur in different experimental conditions.

Research Articles on DNM1

1. dynamin isoforms select appropriate vesicle reuse pathways associated with specific neuronal firing patterns

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DNM1 antibody

Reference Product	PubMed Publications
Sh3gl2 antibody	>1 publications with DNM1 and Sh3gl2

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Diseases associated with anti-DNM1 antibody

Disease Name	Pubmed Publications
Dog Diseases Antibodies	>4 publications with DNM1 and Dog Diseases
Disease Models, Animal Antibodies	>3 publications with DNM1 and Disease Models, Animal
Brain Diseases Antibodies	>3 publications with DNM1 and Brain Diseases
Nervous System Malformations Antibodies	>2 publications with DNM1 and Nervous System Malformations
Kidney Diseases Antibodies	>2 publications with DNM1 and Kidney Diseases
Cognition Disorders Antibodies	>1 publications with DNM1 and Cognition Disorders
Neurobehavioral Manifestations Antibodies	>1 publications with DNM1 and Neurobehavioral Manifestations
Memory Disorders Antibodies	>1 publications with DNM1 and Memory Disorders
Necrosis Antibodies	>1 publications with DNM1 and Necrosis
Fatty Liver Antibodies	>1 publications with DNM1 and Fatty Liver

Organs/Tissues associated with anti-DNM1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>5 publications with DNM1 and Brain
Liver Antibodies	>3 publications with DNM1 and Liver
Connective Tissue Antibodies	>1 publications with DNM1 and Connective Tissue
Spleen Antibodies	>1 publications with DNM1 and Spleen
Eye Antibodies	>1 publications with DNM1 and Eye