GABAA Receptor, G 2 (Ser327) (GABRG2) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Rat

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)

Specific for ~45k GABAA receptor gamma y2 subunit phosphorylated at Ser327 in Western Blots of rat brain homogenate.

For long term storage ~80 degree C is recommended, but shorter term storage at ~20 degree C is also acceptable as aliquots maybe taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

45

Chromosome: 10; NC_005109.3 (26934991..27024440, complement). Location: 10q21

ModBase 3D Structure for P18508

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser327 of the GABAA receptor, gamma2 subunit.

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH

Rat

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, mouse, human and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. It has recently been suggested that PKCepsilon regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)