Rabbit Anti-GLRA1 Polyclonal Antibody – Cat# MBS502066

Glycine Receptor (GLRA1) Antibody – Rabbit Polyclonal

Name: GLRA1 Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502066
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502066

MBS502066 is an antibody targeting GLRA1. Also known as Anti-Glycine Receptor. Reactive in Human, mouse, rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Glycine Receptor (GLRA1), Polyclonal Antibody

Full Product Name

Anti-Glycine Receptor

Product Gene Name

anti-GLRA1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, mouse, rat

Form/Format

200 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody s

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

Specificity

Specific for the ~48k alpha1- and alpha2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

Chromosome Location

Chromosome: 10; NC_005109.3 (40687069..40785100, complement). Location: 10q22

3D Structure

ModBase 3D Structure for P07727

Antigen

Peptide from the N-terminus region of the alpha1-subunit of rat glycine receptor.

Immunogen Information

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Immunogen Species

Rat

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots in human, mouse and rat tissues.

Biological Significance

Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).

Applications Tested/Suitable for anti-GLRA1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-GLRA1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000

Product References and Citations for anti-GLRA1 antibody

• Beckstead MJ, Phelan R, Mihic SJ (2001) Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276:24959-24964.
• Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui JK, Tu SC, Kevin ASK, Nakanishi N, Tong G, Lipton SA, Zhang DX (2002) Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature (London) 415:793-798.
• Craig AM, Lichtman JW (2001) Getting a bead on receptor movements. Nat Neurosci 4:219-220.
• Fischer F, Kneussel M, Tintrup H, Haverkamp S, Rauen T, Betz H, Wässle H (2000) Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427:634-648.
• Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SE, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL (1997) Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature (London) 389:385-389.

Other Notes

Small volumes of anti-GLRA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GLRA1. It may not necessarily be applicable to this product.

NCBI GI #

6980950

NCBI GeneID

25674

NCBI Accession #

NP_037265.1

UniProt Primary Accession #

P07727

UniProt Secondary Accession #

Q546L6

UniProt Related Accession #

P07727

GenBank Nucleotide #

NM_013133.1

NCBI Official Full Name

glycine receptor subunit alpha-1

NCBI Official Synonym Full Names

glycine receptor, alpha 1

NCBI Official Symbol

Glra1

NCBI Official Synonym Symbols

GLYRA1

UniProt Protein Name

Glycine receptor subunit alpha-1

UniProt Synonym Protein Names

Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit

Protein Family

Glycine receptor

UniProt Gene Name

Glra1

UniProt Synonym Gene Names

Glyr

UniProt Entry Name

GLRA1_RAT

NCBI Protein Information

glycine receptor subunit alpha-1; glycine receptor 48 kDa subunit; glycine receptor, alpha 1 subunit; glycine receptor strychnine-binding subunit

NCBI Summary for GLRA1

glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]

UniProt Comments for GLRA1

Function: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing).

Subunit structure: Pentamer composed of three alpha and two beta subunits. Homopentamers of alpha subunits also form functional receptors.

Subcellular location: Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Miscellaneous: The alpha subunit binds strychnine.

Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily. [View classification]

Research Articles on GLRA1

1. overexpressing the Glra1 subunite in rat neurons caused reduced potentition and enhanced inhibition by zinc.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GLRA1 antibody

Products by Pathway	Pathway Diagram
Ion Channel Transport Pathway antibodies	Ion Channel Transport Pathway Diagram
Ligand-gated Ion Channel Transport Pathway antibodies	Ligand-gated Ion Channel Transport Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Transmembrane Transport Of Small Molecules Pathway antibodies	Transmembrane Transport Of Small Molecules Pathway Diagram

Diseases associated with anti-GLRA1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>58 publications with GLRA1 and Nervous System Diseases
Brain Diseases Antibodies	>11 publications with GLRA1 and Brain Diseases
Immune System Diseases Antibodies	>9 publications with GLRA1 and Immune System Diseases
Seizures Antibodies	>3 publications with GLRA1 and Seizures
Neoplasms Antibodies	>2 publications with GLRA1 and Neoplasms
Learning Disorders Antibodies	>2 publications with GLRA1 and Learning Disorders
Poisoning Antibodies	>1 publications with GLRA1 and Poisoning
Hyperexplexia hereditary Antibodies	>1 publications with GLRA1 and Hyperexplexia hereditary
Dystonic Disorders Antibodies	>1 publications with GLRA1 and Dystonic Disorders

Organs/Tissues associated with anti-GLRA1 antibody

Organ/Tissue Name	Pubmed Publications
Mixed Antibodies	>2 publications with GLRA1 and Mixed