Rabbit Anti-GRIN1 Polyclonal Antibody – Cat# MBS502082

NMDA NR1 Subunit, Splice Variant N1 (GRIN1) Antibody – Rabbit Polyclonal

Name: GRIN1 Antibody; Polyclonal; Rabbit; Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502082
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502082

MBS502082 is an antibody targeting GRIN1. Also known as Anti-NMDA Receptor, NR1 Subunit, N1 Splice Variant Insert. Reactive in Mouse, rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NMDA NR1 Subunit, Splice Variant N1 (GRIN1), Polyclonal Antibody

Full Product Name

Anti-NMDA Receptor, NR1 Subunit, N1 Splice Variant Insert

Product Gene Name

anti-GRIN1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Mouse, rat

Form/Format

25 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

Specificity

Specific for the ~120k NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

120

Chromosome Location

Chromosome: 3; NC_005102.3 (2489158..2516082, complement). Location: 3p13

3D Structure

ModBase 3D Structure for P35439

Antigen

Peptide from the NR1 subunit, N1 splice variant insert of rat NMDA receptor.

Immunogen Information

Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Canine, chicken, human, non-human primates and Xenopus

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with canine, chicken, human, non-human primate and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.

Applications Tested/Suitable for anti-GRIN1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-GRIN1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000 to 1:2000

Product References and Citations for anti-GRIN1 antibody

• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.
• Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci 18:306-313.

Other Notes

Small volumes of anti-GRIN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRIN1. It may not necessarily be applicable to this product.

NCBI GI #

396578144

NCBI GeneID

24408

NCBI Accession #

NP_001257531.1

UniProt Primary Accession #

P35439

UniProt Secondary Accession #

Q62646

UniProt Related Accession #

P35439

GenBank Nucleotide #

NM_001270602.1

NCBI Official Full Name

glutamate receptor ionotropic, NMDA 1 isoform 1b

NCBI Official Synonym Full Names

glutamate receptor, ionotropic, N-methyl D-aspartate 1

NCBI Official Symbol

Grin1

NCBI Official Synonym Symbols

NR1; GluN1; NMDAR1

UniProt Protein Name

Glutamate receptor ionotropic, NMDA 1

UniProt Synonym Protein Names

Glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1

Protein Family

Glutamate receptor ionotropic

UniProt Gene Name

Grin1

UniProt Synonym Gene Names

Nmdar1; GluN1; NMD-R1

UniProt Entry Name

NMDZ1_RAT

NCBI Protein Information

glutamate receptor ionotropic, NMDA 1; NMD-R1; neurotransmitter receptor; NMDA R1 receptor C1 cassette; N-methyl-D-aspartate glutamate receptor; glutamate [NMDA] receptor subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1

NCBI Summary for GRIN1

subunit of NMDA-preferring ionotropic glutamate receptors; may play a role in long term potentiation [RGD, Feb 2006]

UniProt Comments for GRIN1

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. Ref.18

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked

By similarity. Found in a complex with GRIN2A or GRIN2B, GRIN3A and PPP2CB. Found in a complex with GRIN2A or GRIN2B and GRIN3B. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes

By similarity. Interacts with DLG4 and MPDZ. Interacts with LRFN1 and LRFN2. Interacts with MYZAP. Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.17

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Cell junction › synapse › postsynaptic cell membrane

By similarity. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Note: Enriched in postsynaptic plasma membrane and postsynaptic densities

By similarity.

Post-translational modification: NMDA is probably regulated by C-terminal phosphorylation of an isoform ofNR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. Ref.7 Ref.12

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. [View classification]

Research Articles on GRIN1

1. These results suggest that PI3K/Akt and NMDAR independently regulate spinal plasticity in visceral pain model

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GRIN1 antibody

Products by Pathway	Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies	CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram

Diseases associated with anti-GRIN1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>22 publications with GRIN1 and Nervous System Diseases
Brain Diseases Antibodies	>12 publications with GRIN1 and Brain Diseases
Disease Models, Animal Antibodies	>7 publications with GRIN1 and Disease Models, Animal
Memory Disorders Antibodies	>5 publications with GRIN1 and Memory Disorders
Nerve Degeneration Antibodies	>5 publications with GRIN1 and Nerve Degeneration
Movement Disorders Antibodies	>4 publications with GRIN1 and Movement Disorders
Learning Disorders Antibodies	>4 publications with GRIN1 and Learning Disorders
Neoplasms Antibodies	>4 publications with GRIN1 and Neoplasms
Pain Antibodies	>4 publications with GRIN1 and Pain
Seizures Antibodies	>3 publications with GRIN1 and Seizures

Organs/Tissues associated with anti-GRIN1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>58 publications with GRIN1 and Brain
Eye Antibodies	>2 publications with GRIN1 and Eye