Rabbit Anti-GRIN2B Polyclonal Antibody – Cat# MBS502089

NMDA NR2B Subunit (Tyr1252) (GRIN2B) Antibody – Rabbit Polyclonal

Name: GRIN2B Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502089
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502089

MBS502089 is an antibody targeting GRIN2B. Also known as Anti-Phospho-Tyr1252 NMDA Receptor NR2B Subunit. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

NMDA NR2B Subunit (Tyr1252) (GRIN2B), Polyclonal Antibody

Full Product Name

Anti-Phospho-Tyr1252 NMDA Receptor NR2B Subunit

Product Gene Name

anti-GRIN2B antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for ~180k NMDAR NR2B subunit protein phosphorylated at Tyr1252. Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. Immunolabeling is also blocked by lambda-phosphatase treatment. The antibody may also show some slight reactivity with Tyr1246 of NR2A.

Preparation and Storage

Store at -20 degree C; stable for at least one year

Molecular Weight

180

Chromosome Location

Chromosome: 4; NC_005103.3 (233806406..234260360, complement). Location: 4q43

3D Structure

ModBase 3D Structure for Q00960

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1252 of the NR2B subunit of the rat NMDA receptor.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Bovine, canine, chicken, human, mouse, non-human primate and zebra fish

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr1252 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002).

Applications Tested/Suitable for anti-GRIN2B antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-GRIN2B antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:400

Product References and Citations for anti-GRIN2B antibody

• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843.
• Takasu, MA, Dalva, MB, Zigmond, RE, Greenberg, ME (2002) Modulation of NMDA Receptor -Dependent Calcium Influx and Gene Expression Through EphB Receptors. Science 295:491-495.
• Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358.

Other Notes

Small volumes of anti-GRIN2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRIN2B. It may not necessarily be applicable to this product.

NCBI GI #

6980984

NCBI GeneID

24410

NCBI Accession #

NP_036706.1

UniProt Primary Accession #

Q00960

UniProt Secondary Accession #

Q62684

UniProt Related Accession #

Q00960

GenBank Nucleotide #

NM_012574.1

NCBI Official Full Name

glutamate receptor ionotropic, NMDA 2B

NCBI Official Synonym Full Names

glutamate receptor, ionotropic, N-methyl D-aspartate 2B

NCBI Official Symbol

Grin2b

NCBI Official Synonym Symbols

GluN2B

UniProt Protein Name

Glutamate receptor ionotropic, NMDA 2B

UniProt Synonym Protein Names

Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B

Protein Family

Glutamate receptor ionotropic

UniProt Gene Name

Grin2b

UniProt Synonym Gene Names

GluN2B; NMDAR2B; NR2B

UniProt Entry Name

NMDE2_RAT

NCBI Protein Information

glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2

NCBI Summary for GRIN2B

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

UniProt Comments for GRIN2B

Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death

By similarity.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3. Interacts with HIP1 and Neto1

By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with DAPK1

By similarity. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Post-translational modification: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity

By similarity.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. [View classification]

Research Articles on GRIN2B

1. Artificial food colors and additives led to an increase in NR2B and nAChR beta2 receptor subunits in male rats and led to decrease in NR2B subunits in female rats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GRIN2B antibody

Reference Product	PubMed Publications
Grin2a antibody	>30 publications with GRIN2B and Grin2a
Grin1 antibody	>21 publications with GRIN2B and Grin1
Grik2 antibody	>5 publications with GRIN2B and Grik2
Grin3b antibody	>3 publications with GRIN2B and Grin3b
Grin3a antibody	>2 publications with GRIN2B and Grin3a
Grik1 antibody	>2 publications with GRIN2B and Grik1
Dlg4 antibody	>1 publications with GRIN2B and Dlg4

Pathways associated with anti-GRIN2B antibody

Products by Pathway	Pathway Diagram
Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies	Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alcoholism Pathway antibodies	Alcoholism Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Alzheimer's Disease Pathway antibodies	Alzheimer's Disease Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amphetamine Addiction Pathway antibodies	Amphetamine Addiction Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies	CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram

Diseases associated with anti-GRIN2B antibody

Disease Name	Pubmed Publications
Schizophrenia Antibodies	>39 publications with GRIN2B and Schizophrenia
Nervous System Diseases Antibodies	>28 publications with GRIN2B and Nervous System Diseases
Brain Diseases Antibodies	>17 publications with GRIN2B and Brain Diseases
Anxiety Disorders Antibodies	>10 publications with GRIN2B and Anxiety Disorders
Movement Disorders Antibodies	>10 publications with GRIN2B and Movement Disorders
Child Development Disorders, Pervasive Antibodies	>9 publications with GRIN2B and Child Development Disorders, Pervasive
Neoplasms Antibodies	>8 publications with GRIN2B and Neoplasms
Cognition Disorders Antibodies	>8 publications with GRIN2B and Cognition Disorders
Epilepsy Antibodies	>8 publications with GRIN2B and Epilepsy
Intellectual Disability Antibodies	>7 publications with GRIN2B and Intellectual Disability