Rabbit Anti-RAB3A Polyclonal Antibody – Cat# MBS502107

RAB3A Antibody – Rabbit Polyclonal

Name: RAB3A Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502107
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502107

MBS502107 is an antibody targeting RAB3A. Also known as Anti-Rab 3. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Rab 3 (RAB3A), Polyclonal Antibody

Full Product Name

Anti-Rab 3

Product Gene Name

anti-RAB3A antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.)

Specificity

Specific for ~27k Rab3 protein.

Preparation and Storage

Store at -20 degree C; stable for at least one year.

Molecular Weight

Chromosome Location

Chromosome: 16; NC_005115.3 (20292166..20296274, complement). Location: 16p14

3D Structure

ModBase 3D Structure for P63012

Antigen

Peptide corresponding to amino acid residues from the N terminal region of rat Rab 3, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Information

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Human, mouse, Xenopus, and non-human primates

Species Reactivity Note

The antibody has been demonstrated to work in rat tissue. It is anticipated that the antibody will work in human, mouse, Xenopus, and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has 4 known isoforms: 3A, 3B, 3C, and 3D (Schluter et al., 2004). These proteins are expressed in highest concentrations in brain and endocrine tissues and involved in regulated exocytosis of hormones and neurotransmitters (Coppola et al., 2002). Specifically, Rab3's are thought to play a key role in presynaptic vesicle trafficking and priming (Schluter et al., 2006).

Applications Tested/Suitable for anti-RAB3A antibody

Western Blot (WB)

Application Notes for anti-RAB3A antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-RAB3A antibody

• Schluter OM, Schmitz F, Jahn R, Rosenmund C, Sudhof TC (2004) A Complete Genetic Analysis of Neuronal Rab3 Function. J Neuroscience 24(29):6629-6637
• Schluter OM, Basu J, Sudhof TC, Rosenmund C (2006) Rab3 Superprimes Synaptic Vesicles for Release: Implications for Short-term Synaptic Plasticity. J Neuroscience 26(4):1239-1246.
• Coppola T, Perret-Menoud V, Gattesco S, Magnin S, Pombo I, Blank U, Regazzi R (2002) The death domain of Rab3 guanine nucleotide exchange protein in GDP/GTP exchange activity in living cells. J Biochem 362 (Pt.2):273-9.

Other Notes

Small volumes of anti-RAB3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RAB3A. It may not necessarily be applicable to this product.

NCBI GI #

61098195

NCBI GeneID

25531

NCBI Accession #

NP_037150.2

UniProt Primary Accession #

P63012

UniProt Secondary Accession #

P05713

UniProt Related Accession #

P63012

GenBank Nucleotide #

NM_013018.2

NCBI Official Full Name

ras-related protein Rab-3A

NCBI Official Synonym Full Names

RAB3A, member RAS oncogene family

NCBI Official Symbol

Rab3a

NCBI Official Synonym Symbols

RAB3

UniProt Protein Name

Ras-related protein Rab-3A

Protein Family

Ras-related protein

UniProt Gene Name

Rab3a

UniProt Entry Name

RAB3A_RAT

NCBI Protein Information

ras-related protein Rab-3A; Ras-related small GTP binding protein 3A

NCBI Summary for RAB3A

regulated synaptic vesicle endocytosis [RGD, Feb 2006]

UniProt Comments for RAB3A

Function: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal

By similarity.

Subunit structure: Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4 and RIMS1. Interacts with SGSM1 and SGSM3

By similarity. Heterodimer with RIMS2. Interacts with RPH3A. Interacts with RAB3IP. Ref.4 Ref.5 Ref.6

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side

Potential.

Tissue specificity: Detected in brain.

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.

Research Articles on RAB3A

1. the Rab3A cycle is coupled with the activation of Munc13-1 via RIM, which accounts for the regulation of secretion by Rab3A.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-RAB3A antibody

Products by Pathway	Pathway Diagram
Acetylcholine Neurotransmitter Release Cycle Pathway antibodies	Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies	GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Glutamate Neurotransmitter Release Cycle Pathway antibodies	Glutamate Neurotransmitter Release Cycle Pathway Diagram
Insulin Secretion Pathway antibodies	Insulin Secretion Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Release Cycle Pathway antibodies	Neurotransmitter Release Cycle Pathway Diagram
Norepinephrine Neurotransmitter Release Cycle Pathway antibodies	Norepinephrine Neurotransmitter Release Cycle Pathway Diagram
Synaptic Vesicle Cycle Pathway antibodies	Synaptic Vesicle Cycle Pathway Diagram
Synaptic Vesicle Cycle Pathway antibodies	Synaptic Vesicle Cycle Pathway Diagram
Transmission Across Chemical Synapses Pathway antibodies	Transmission Across Chemical Synapses Pathway Diagram

Diseases associated with anti-RAB3A antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>42 publications with RAB3A and Nervous System Diseases
Brain Diseases Antibodies	>28 publications with RAB3A and Brain Diseases
Neurodegenerative Diseases Antibodies	>19 publications with RAB3A and Neurodegenerative Diseases
Disease Models, Animal Antibodies	>7 publications with RAB3A and Disease Models, Animal
Nerve Degeneration Antibodies	>6 publications with RAB3A and Nerve Degeneration
Movement Disorders Antibodies	>6 publications with RAB3A and Movement Disorders
Neurobehavioral Manifestations Antibodies	>5 publications with RAB3A and Neurobehavioral Manifestations
Kidney Diseases Antibodies	>4 publications with RAB3A and Kidney Diseases
Immune System Diseases Antibodies	>3 publications with RAB3A and Immune System Diseases
Atrophy Antibodies	>3 publications with RAB3A and Atrophy

Organs/Tissues associated with anti-RAB3A antibody

Organ/Tissue Name	Pubmed Publications
Nerve Antibodies	>239 publications with RAB3A and Nerve
Brain Antibodies	>224 publications with RAB3A and Brain
Embryonic Tissue Antibodies	>19 publications with RAB3A and Embryonic Tissue
Adrenal Gland Antibodies	>18 publications with RAB3A and Adrenal Gland
Muscle Antibodies	>14 publications with RAB3A and Muscle
Liver Antibodies	>7 publications with RAB3A and Liver
Heart Antibodies	>7 publications with RAB3A and Heart
Eye Antibodies	>6 publications with RAB3A and Eye
Connective Tissue Antibodies	>5 publications with RAB3A and Connective Tissue
Pineal Gland Antibodies	>1 publications with RAB3A and Pineal Gland