Rabbit Anti-RPH3A Polyclonal Antibody – Cat# MBS502108

Rabphilin 3a (RPH3A) Antibody – Rabbit Polyclonal

Name: RPH3A Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502108
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502108

MBS502108 is an antibody targeting RPH3A. Also known as Anti-Rabphilin 3A. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Rabphilin 3A (RPH3A), Polyclonal Antibody

Full Product Name

Anti-Rabphilin 3A

Product Gene Name

anti-RPH3A antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification on a column to which the C-terminal peptide immunogen was coupled.)

Specificity

Specific for the ~82k rabphilin 3A protein

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

Chromosome Location

Chromosome: 12; NC_005111.3 (42938832..43014142). Location: 12q16

3D Structure

ModBase 3D Structure for P47709

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rabphilin 3A.

Immunogen Information

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Mouse, human, bovine, canine and non-human primate

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, human, bovine, canine and non-human primate tissue based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease (Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).

Applications Tested/Suitable for anti-RPH3A antibody

Western Blot (WB)

Application Notes for anti-RPH3A antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-RPH3A antibody

• Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.
• Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.
• Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond) 369:493-497.
• Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman ER, Jahn R, De Camilli P, Südhof TC (1994) Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid- binding protein, depends on rab3A/3C. Neuron 13:885-898.
• Smith R, Petersen A, Bates GP, Brundin P, Li JY (2005) Depletion of rabphilin 3A in a transgenic mouse model (R6/1) of Huntington's disease, a possible culprit in synaptic dysfunction. Neurobiol Dis 20:673-684.

Other Notes

Small volumes of anti-RPH3A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for RPH3A. It may not necessarily be applicable to this product.

NCBI GI #

19424162

NCBI GeneID

171039

NCBI Accession #

NP_598202.1

UniProt Primary Accession #

P47709

UniProt Related Accession #

P47709

GenBank Nucleotide #

NM_133518.1

NCBI Official Full Name

rabphilin-3A

NCBI Official Synonym Full Names

rabphilin 3A

NCBI Official Symbol

Rph3a

UniProt Protein Name

Rabphilin-3A

UniProt Synonym Protein Names

Exophilin-1

Protein Family

Rabphilin

UniProt Gene Name

Rph3a

UniProt Entry Name

RP3A_RAT

NCBI Protein Information

rabphilin-3A; exophilin-1; rabphilin 3A homolog

NCBI Summary for RPH3A

binds Ca2+ and phospholipids [RGD, Feb 2006]

UniProt Comments for RPH3A

Function: Protein transport. Probably involved with Ras-related protein Rab-3A in synaptic vesicle traffic and/or synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal.

Subunit structure: Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B

By similarity. Interacts with RAB3A. Monomer.

Subcellular location: Cell junction › synapse

By similarity. Membrane; Peripheral membrane protein Ref.4.

Tissue specificity: Specifically expressed in brain.

Domain: Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers. Ref.4

Sequence similarities: Contains 2 C2 domains.Contains 1 FYVE-type zinc finger.Contains 1 RabBD (Rab-binding) domain.

Research Articles on RPH3A

1. Binding of rabphilin to SNAP-25 regulates exocytosis of synaptic vesicles after the readily releasable pool has either been physiologically exhausted by use-dependent depression, or has been artificially depleted by deletion of synaptobrevin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-RPH3A antibody

Reference Product	PubMed Publications
Snap25 antibody	>1 publications with RPH3A and Snap25
Rab3a antibody	>1 publications with RPH3A and Rab3a