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Synapsin (Ser62,67) (SYN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502116

MBS502116 is an antibody targeting SYN1. Also known as Anti-Phospho-Ser62,67 Synapsin I. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Synapsin (Ser62,67) (SYN1), Polyclonal Antibody
Full Product Name
Anti-Phospho-Ser62,67 Synapsin I
Product Gene Name
anti-SYN1 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Specificity
Specific for ~78k synapsin I doublet phosphorylated at Ser62,67. Immunolabeling of the synapsin I band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide..
Preparation and Storage
Store at -20 degree C; stable for at least one year
Molecular Weight
78
Chromosome Location
Chromosome: X; AC_000187.1 (91192243..91229595, complement). Location: chromosome: X
3D Structure
ModBase 3D Structure for P17599
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62,67 of synapsin I.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Mouse, and bovine
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, and bovine tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).
Applications Tested/Suitable for anti-SYN1 antibody
Western Blot (WB)
Application Notes for anti-SYN1 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-SYN1 antibody

Rabbit Anti-SYN1 Polyclonal Antibody – Cat# MBS502116

Product References and Citations for anti-SYN1 antibody

• Jovanovic JN, Benfenati, F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P,Czernik AJ (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate Synapsin I-actin interactions. Neurobiology 93:3679-3683.
• Czernik AJ, Pang DT, Greengard P (1987) Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I. Proc Natl Acad Sci (USA) 84:7518-7522.
• Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380.
• Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953.
• Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.

Other Notes

Small volumes of anti-SYN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SYN1. It may not necessarily be applicable to this product.

NCBI GI #
27806393
NCBI GeneID
281510
NCBI Accession #
NP_776616.1
UniProt Primary Accession #
P17599
UniProt Related Accession #
P17599
GenBank Nucleotide #
NM_174191.2
NCBI Official Full Name
synapsin-1
NCBI Official Symbol
SYN1
UniProt Protein Name
Synapsin-1
UniProt Synonym Protein Names
Synapsin I
Protein Family
Sister chromatid cohesion 1 protein
UniProt Gene Name
SYN1
UniProt Entry Name
SYN1_BOVIN
NCBI Protein Information
synapsin-1

UniProt Comments for SYN1

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level

By similarity.

Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP

By similarity.

Subcellular location: Cell junction › synapse. Golgi apparatus

By similarity.

Domain: The A region binds phospholipids with a preference for negatively charged species

By similarity.

Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles

By similarity.

Sequence similarities: Belongs to the synapsin family.
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Research Articles on SYN1

1. These results raise the possibility that Ser(603) on synapsin I is alternatively phosphorylated by p21-activated kinases, not only by CaMKII, in neuronal cells in response to some stimulants.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-SYN1 antibody

 Disease Name  Pubmed Publications
 Brain Diseases Antibodies  >11 publications with SYN1 and Brain Diseases
 Mental Disorders Antibodies  >11 publications with SYN1 and Mental Disorders
 Central Nervous System Diseases Antibodies  >9 publications with SYN1 and Central Nervous System Diseases
 Neoplasms Antibodies  >6 publications with SYN1 and Neoplasms
 Cognition Disorders Antibodies  >4 publications with SYN1 and Cognition Disorders
 Neurobehavioral Manifestations Antibodies  >4 publications with SYN1 and Neurobehavioral Manifestations
 Seizures Antibodies  >3 publications with SYN1 and Seizures
 Learning Disorders Antibodies  >3 publications with SYN1 and Learning Disorders
 Chromosome Aberrations Antibodies  >3 publications with SYN1 and Chromosome Aberrations
 Necrosis Antibodies  >3 publications with SYN1 and Necrosis

Organs/Tissues associated with anti-SYN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >29 publications with SYN1 and Brain
 Kidney Antibodies  >1 publications with SYN1 and Kidney
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