Rabbit Anti-TPH1 Polyclonal Antibody – Cat# MBS502129

Tryptophan Hydroxylase (Ser58) (TPH1) Antibody – Rabbit Polyclonal

Name: TPH1 Antibody; Polyclonal; Rabbit; Rabbit-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502129
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502129

MBS502129 is an antibody targeting TPH1. Also known as Anti-Phospho-Ser58 Tryptophan Hydroxylase 1. Reactive in Rabbit. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Tryptophan Hydroxylase (Ser58) (TPH1), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser58 Tryptophan Hydroxylase 1

Product Gene Name

anti-TPH1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rabbit

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

Chromosome Location

Chromosome: 1; NC_005100.3 (103746878..103774100, complement). Location: 1q22

3D Structure

ModBase 3D Structure for P09810

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of tryptophan Hydroxylase 1 (TPH1).

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Bovine chicken, human, mouse non-human primate, rat xenopus zebra fish

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rabbit tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primate, rat, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Applications Tested/Suitable for anti-TPH1 antibody

Western Blot (WB)

Application Notes for anti-TPH1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-TPH1 antibody

• Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017.
• Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823.
• Kuhn, DM, Arthur, Jr, R, States, JC (1997) Phosphorylation and activation of brain tryptophan hydroxylase: identification of serine-58 as a substrate site for protein kinase A. J Neurochem 68:2220-2223.
• Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.

Other Notes

Small volumes of anti-TPH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TPH1. It may not necessarily be applicable to this product.

NCBI GI #

158186610

NCBI GeneID

24848

NCBI Accession #

NP_001094104.1

UniProt Primary Accession #

P09810

UniProt Related Accession #

P09810

GenBank Nucleotide #

NM_001100634.2

NCBI Official Full Name

tryptophan 5-hydroxylase 1

NCBI Official Synonym Full Names

tryptophan hydroxylase 1

NCBI Official Symbol

Tph1

NCBI Official Synonym Symbols

Tph

UniProt Protein Name

Tryptophan 5-hydroxylase 1

UniProt Synonym Protein Names

Tryptophan 5-monooxygenase 1

Protein Family

Tryptophan 5-hydroxylase

UniProt Gene Name

Tph1

UniProt Synonym Gene Names

Tph

UniProt Entry Name

TPH1_RAT

NCBI Protein Information

tryptophan 5-hydroxylase 1; tryptophan 5-monooxygenase 1

NCBI Summary for TPH1

catalyzes the conversion of l-tryptophan to 5-hydroxy-l-tryptophan; member of pterin-dependent aromatic amino acid hydroxylase family [RGD, Feb 2006]

UniProt Comments for TPH1

Catalytic activity: L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin.

Cofactor: Fe2+ ion.

Pathway: Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.

Subunit structure: Homotetramer.

Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.Contains 1 ACT domain.

Research Articles on TPH1

1. An increased expression of TPH1 in the dorsal raphe nucleus may be the primary mechanism to explain the chronic increase in the brain synthesis of 5-HT in these in utero malnourished rats.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TPH1 antibody

Reference Product	PubMed Publications
Tph2 antibody	>117 publications with TPH1 and Tph2
Ddc antibody	>4 publications with TPH1 and Ddc
Ido2 antibody	>1 publications with TPH1 and Ido2
Ido1 antibody	>1 publications with TPH1 and Ido1

Pathways associated with anti-TPH1 antibody

Products by Pathway	Pathway Diagram
Amine-derived Hormones Pathway antibodies	Amine-derived Hormones Pathway Diagram
Biogenic Amine Synthesis Pathway antibodies	Biogenic Amine Synthesis Pathway Diagram
Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway antibodies	Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway Diagram
Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway antibodies	Melatonin Biosynthesis, Tryptophan => Serotonin => Melatonin Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Amino Acids And Derivatives Pathway antibodies	Metabolism Of Amino Acids And Derivatives Pathway Diagram
Serotonergic Synapse Pathway antibodies	Serotonergic Synapse Pathway Diagram
Serotonin And Melatonin Biosynthesis Pathway antibodies	Serotonin And Melatonin Biosynthesis Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram
Tryptophan Metabolism Pathway antibodies	Tryptophan Metabolism Pathway Diagram

Diseases associated with anti-TPH1 antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>24 publications with TPH1 and Inflammation
Schizophrenia Antibodies	>22 publications with TPH1 and Schizophrenia
Hypertension Antibodies	>17 publications with TPH1 and Hypertension
Hypertension, Pulmonary Antibodies	>17 publications with TPH1 and Hypertension, Pulmonary
Nervous System Diseases Antibodies	>15 publications with TPH1 and Nervous System Diseases
Neoplasms Antibodies	>13 publications with TPH1 and Neoplasms
Necrosis Antibodies	>7 publications with TPH1 and Necrosis
Cardiovascular Diseases Antibodies	>7 publications with TPH1 and Cardiovascular Diseases
Immune System Diseases Antibodies	>7 publications with TPH1 and Immune System Diseases
Heart Diseases Antibodies	>5 publications with TPH1 and Heart Diseases

Organs/Tissues associated with anti-TPH1 antibody

Organ/Tissue Name	Pubmed Publications
Pineal Gland Antibodies	>19 publications with TPH1 and Pineal Gland