Goat Anti-CHAT Polyclonal Antibody – Cat# MBS502147

CHAT Antibody – Goat Polyclonal

Name: CHAT Antibody; Polyclonal; Goat; MyBioSource
Brand: MyBioSource
SKU: MBS502147
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502147

MBS502147 is an antibody targeting CHAT. Also known as Anti-Choline Acetyltransferase. Reactive in Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates. Host: Goat. Affinity purified goat polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ChAT (Choline Acetyltransferase), Polyclonal Antibody

Full Product Name

Anti-Choline Acetyltransferase

Product Gene Name

anti-CHAT antibody

Clonality

Polyclonal

Host

Goat

Species Reactivity

Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates

Form/Format

100 ul in PBS + 5 mg/ml BSA + 0.2% NaN3.. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.)

Specificity

Specific for the ~ 70k choline acetyltransferase protein

Preparation and Storage

For long term storage -20 degree C is recommended. Stable for at least 1 year.

Molecular Weight

Chromosome Location

Chromosome: 10; NC_000010.11 (49609095..49665104). Location: 10q11.2

3D Structure

ModBase 3D Structure for P28329

Antigen

Native choline acetyltransferase purified from human placenta

Immunogen Information

Native choline acetyltransferase purifed from human placenta

Immunogen Species

Human

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with chicken, guinea pig, human, mouse, non-human primate and rat tissues.

Biological Significance

Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Applications Tested/Suitable for anti-CHAT antibody

Western Blot (WB)

Application Notes for anti-CHAT antibody

Quality Control: Western blots performed on each lot.
WB: 1:100

Product References and Citations for anti-CHAT antibody

• Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.
• Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.
• Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.

Other Notes

Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.

NCBI GeneID

1103

UniProt Primary Accession #

P28329

UniProt Related Accession #

P28329

NCBI Official Synonym Full Names

choline O-acetyltransferase

NCBI Official Symbol

CHAT

NCBI Official Synonym Symbols

CMS1A; CMS1A2; CHOACTASE

Protein Family

(Z)-3-hexen-1-ol acetyltransferase

NCBI Protein Information

choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase

NCBI Summary for CHAT

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

UniProt Comments for CHAT

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.6

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; cytosol; nucleus

Molecular Function: choline O-acetyltransferase activity

Biological Process: synaptic transmission; rhythmic excitation; muscle development; rhythmic behavior; phospholipid metabolic process; neurotransmitter secretion; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

Research Articles on CHAT

1. rs1880676 is functional and the allelic variations of this polymorphism are involved in developing nicotine dependence by altering ChAT expression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CHAT antibody

Products by Pathway	Pathway Diagram
Acetylcholine Neurotransmitter Release Cycle Pathway antibodies	Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
Acetylcholine Synthesis Pathway antibodies	Acetylcholine Synthesis Pathway Diagram
Biogenic Amine Synthesis Pathway antibodies	Biogenic Amine Synthesis Pathway Diagram
Cholinergic Synapse Pathway antibodies	Cholinergic Synapse Pathway Diagram
Glycerophospholipid Biosynthesis Pathway antibodies	Glycerophospholipid Biosynthesis Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram
Glycerophospholipid Metabolism Pathway antibodies	Glycerophospholipid Metabolism Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Lipids And Lipoproteins Pathway antibodies	Metabolism Of Lipids And Lipoproteins Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram