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fragment of ACTIN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502149

MBS502149 is an antibody targeting fragment of Actin. Also known as Anti-Fractin. Reactive in Human, rat. Host: Rabbit. Rabbit polyclonal antibody More details

$435 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fractin (fragment of Actin), Polyclonal Antibody
Full Product Name
Anti-Fractin
Product Gene Name
anti-fragment of Actin antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, rat
Form/Format
Liquid. Contains no preservative.
Purity/Purification
Neat Serum (neat serum.)
Specificity
Specific for the ~ 32 kDa fractin protein in Western blots with no reactivity to intact actin. There is often a ladder of smaller bands in cells or culture or in vivo preparations due to further degradation by other proteases.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year from date of receipt. Avoid freeze/thaw cycles.
Molecular Weight
32
Antigen
Synthetic peptide taken from the C terminus of the 32-kDa actin fragment.
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the C terminal region of the 32-kDa actin fragment
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in human and rat.
Biological Significance
Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer's disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).
Applications Tested/Suitable for anti-fragment of Actin antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-fragment of Actin antibody
Quality Control: Western blots performed on each lot.
WB: 1:1,000
IHC: 1:100

Testing Data of anti-fragment of Actin antibody

Rabbit Anti-fragment of ACTIN Polyclonal Antibody – Cat# MBS502149

Product References and Citations for anti-fragment of Actin antibody

• Yang, F, et al., Antibody to Caspase-Cleaved Actin Detects Apoptosis in Differentiated Neuroblastoma and Plaque-Associated Neurons and Microglia in Alzheimer's disease. American Journal of Pathology, 1998, Vol. 152, No. 2, p. 379-389.
• Rossiter, JP, et al., Caspase-cleaved actin (fractin) immunolabeling of Hirano bodies. Neuropathol Appl Neurobiol. 2000 Aug; 26(4):342-6.
• Asamec, E, et al., Multiple-label immunocytochemistry for the evaluation of nature of cell death in experimental models of neurodegeneration. Brain Res Protoc. 2001 Jul;7(3):193-202.
• Chen, TA, et al., Inhibition of caspase-3-like activity reduces glutamate induced cell death in adult rat retina. Brain Res. 2001 Jun 15;904(1):177-88.
• Rossiter, JP, et al., Caspase-3 activation and caspase-like proteolytic activity in human perinatal hypoxic-ischemic brain injury. Acta Neuropathol. 2002 Jan;103(1):66-73.

Other Notes

Small volumes of anti-fragment of Actin antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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